Diverse Functions of Il-2, Il-15, and Il-7 in Lymphoid Homeostasis

Ma, Averil; Koka, Rima; Burkett, Patrick R.

doi:10.1146/annurev.immunol.24.021605.090727

Cited by 608 publications

(595 citation statements)

References 104 publications

Supporting

Mentioning

559

Contrasting

Unclassified

Order By: Relevance

“…Many studies have demonstrated that anti-tumor immunity and the ability to break tolerance could be enhanced by co-delivering additional immunomodulator genes, such as interleukin-2, interleukin-12, interleukin-15, CD70 and other activators, to DCs. 5,[57][58][59][60] We are currently conducting experiments to evaluate the anti-tumor immunity generated by the co-delivery of a tumor antigen and an immuomodulator using this DC-directed lentivector system.…”

Section: Discussionmentioning

confidence: 99%

Dendritic cell-directed lentivector vaccine induces antigen-specific immune responses against murine melanoma

Yang

Liang

et al. 2011

Cancer Gene Ther

View full text Add to dashboard Cite

Lentivectors are potential vaccine delivery vehicles because they can efficiently transduce a variety of non-dividing cells, including antigen-presenting cells, and do not cause expression of extra viral proteins. To improve safety while retaining efficiency, a dendritic cell (DC)-specific lentivector was constructed by pseudotyping the vector with an engineered viral glycoprotein derived from Sindbis virus. We assessed the level of anti-tumor immunity conferred by this engineered lentivector encoding the melanoma antigen gp100 in a mouse model. Footpad injection of the engineered lentivectors results in the best antigen-specific immune response as compared with subcutaneous and intraperitoneal injections. A single prime vaccination of the engineered lentivectors can elicit a high frequency (up to 10%) of gp100-specific CD8 þ T cells in peripheral blood 3 weeks after the vaccination and this response will be maintained at around 5% for up to 8 weeks. We found that these engineered lentivectors elicited relatively low levels of anti-vector neutralizing antibody responses. Importantly, direct injection of this engineered lentivector inhibited the growth of aggressive B16 murine melanoma. These data suggest that DC-specific lentivectors can be a novel and alternative vaccine carrier with the potential to deliver effective anti-tumor immunity for cancer immunotherapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Dendritic cell-directed lentivector vaccine induces antigen-specific immune responses against murine melanoma

Yang

Liang

et al. 2011

Cancer Gene Ther

View full text Add to dashboard Cite

show abstract

“…Activated OT-II cells expressed the subunits a (CD25) and b (CD122) of the IL-2 receptor at the time of adoptive transfer, whereas naive OT-II did not. The source of the IL-2 is clearly the TT-specific CD4 1 T cells [22], where production is usually accompanied by upregulation of the subunits a and b of IL-2R [22] and would be followed by autocrine and/or paracrine consumption of the cytokine [22]. IL-2 is therefore a strong candidate cytokine for driving proliferation of the injected OT-II cells.…”

Section: Discussionmentioning

confidence: 99%

Bystander stimulation of activated CD4⁺ T cells of unrelated specificity following a booster vaccination with tetanus toxoid

et al. 2010

View full text Add to dashboard Cite

Antigen-specific CD4 1 T cells are central to natural and vaccine-induced immunity. An ongoing antigen-specific T-cell response can, however, influence surrounding T cells with unrelated antigen specificities. We previously observed this bystander effect in healthy human subjects following recall vaccination with tetanus toxoid (TT). Since this interplay could be important for maintenance of memory, we have moved to a mouse model for further analysis. We investigated whether boosting memory CD4 1 T cells against TT in vivo would influence injected CD4 1 TCR transgenic T cells (OT-II) specific for an unrelated OVA peptide. If OT-II cells were pre-activated with OVA peptide in vitro, these cells showed a bystander proliferative response during the ongoing parallel TT-specific response. Bystander proliferation was dependent on boosting of the TT-specific memory response in the recipients, with no effect in naive mice. Bystander stimulation was also proportional to the strength of the TT-specific memory T-cell response. T cells activated in vitro displayed functional receptors for IL-2 and IL-7, suggesting these as potential mediators. This crosstalk between a stimulated CD4 1 memory T-cell response and CD4 1 T cells activated by an unrelated antigen could be important in human subjects continually buffeted by environmental antigens.

show abstract

“…IL-2 is a proinflammatory cytokine and one of the most essential cytokines for regulating activation and proliferation of t-cells [20]. The TGF-β1 protein family is involved in muscle growth and atrophy [21] and has been implicated in both the inhibition and activation of t-cells [22,23].…”

Section: Introductionmentioning

confidence: 99%

Serum from exercising humans suppresses t-cell cytokine production

Radom‐Aizik¹,

Leu²,

Zaldivar³

2007

Cytokine

View full text Add to dashboard Cite

Exercise affects t-cell cytokine production. Whether or not these effects are caused by circulating factors associated with physical activity (e.g., inflammatory mediators, acidosis) is unknown. To investigate this, we incubated sera (10%), obtained from 16 young-adults before (PRE) and after (END) 30-min of exercise, with commercially available Jurkat cells, a t-lymphocyte model, that, of course, had never been exposed to an exercise milieu. After 1-h and 6-h in culture, we measured in the supernatant four cytokines (each known to be altered by exercise and involved in disease pathophysiology): IL-2, TGF-β1, TNF-α, and IL-1ra. Cell proliferation was assessed with proliferating nuclear cell antigen (PNCA). Statistical analysis consisted of a linear mixed model for repeated measurement. There was no effect of exercise on t-cell production of either TGF-β1 or IL-1ra. In contrast, both IL-2 (p=0.025) and TNF-α (p=0.031) production was significantly suppressed in sera from the exercising participants. The suppression of these two cytokines occurred despite the fact that PNCA significantly increased (p<0.0001) in the END serum. In conclusion, exercise alters circulating factors that can, subsequently, influence t-cell cytokine production in vitro.

show abstract

Diverse Functions of Il-2, Il-15, and Il-7 in Lymphoid Homeostasis

Cited by 608 publications

References 104 publications

Dendritic cell-directed lentivector vaccine induces antigen-specific immune responses against murine melanoma

Dendritic cell-directed lentivector vaccine induces antigen-specific immune responses against murine melanoma

Bystander stimulation of activated CD4⁺ T cells of unrelated specificity following a booster vaccination with tetanus toxoid

Serum from exercising humans suppresses t-cell cytokine production

Contact Info

Product

Resources

About

Diverse Functions of Il-2, Il-15, and Il-7 in Lymphoid Homeostasis

Cited by 608 publications

References 104 publications

Dendritic cell-directed lentivector vaccine induces antigen-specific immune responses against murine melanoma

Dendritic cell-directed lentivector vaccine induces antigen-specific immune responses against murine melanoma

Bystander stimulation of activated CD4+ T cells of unrelated specificity following a booster vaccination with tetanus toxoid

Serum from exercising humans suppresses t-cell cytokine production

Contact Info

Product

Resources

About

Bystander stimulation of activated CD4⁺ T cells of unrelated specificity following a booster vaccination with tetanus toxoid