Diverse Distribution of Red Cells and Albumin in the Dog Kidney

Abstract

labeled red cells mid I 131 huniiin serum iilbumin were injected intrnvenously into S mongrel dogs. After 1 hour kidneys were removed and frozen rapidly with dry ice. Sections of outer cortex, inner cortex, outer medulla, inner niedulhi, outer pnpilln nnd inner papilla were removed and analyzed for Cr 111 and I 181 radioactivity. Tissue mdioactivity was compared with that of arterial blond and concentrations of labeled red cells and albumin were calculated. No significant difference was found in relative red cell and albumin content between outer and inner cortex. The magnitude of the I 131 albumin content in the cortex Ls interpreted to indicate a sizeable exlruvusvukir exchangeable albumin pool. The renal papillae were extraordinarily deficient in red cells, but contained per ]00 Gin., albumin equivalent to that of 39 ml. of plasma. SKI C!n nlnlion Rrwarrh, Va/ttmr VI. Xorrmbtr at University

Effect of hemorrhage and retransfusion on intrarenal distribution of blood flow in dogs

“…An average cortical red cell volume of 8%o (17)(18)(19)(20) Fig. 7, which includes four measurements during control and retransfusion in addition to those given in Table III blood volume to be constant.…”

Systemic and Renal Hemodynamics in Oliguric Hepatic Failure: Effect of Volume Expansion*

“…A falsely high value for RBF would also result if indocyanine green-labeled albumin equilibrated in a single renal circulation with an extravascular albumin compartment. Such a "loss" of intravascular albumin does not normally occur (20), but abnormally rapid extravascular distribution of albumin in these patients cannot be ruled out. Indeed, patient 21 had a somewhat larger calculated vascular volume than the other patients in this series, suggesting the possibility of an unusually large albumin space.…”

Distribution of chylomicrons and albumin in dog kidney

“…In comparison, several investigators found the sum of renal distribution spaces of labelled red blood cells (R.B.C.s) and albumin to be more than twice this value. Thus Lilienfield, Rose & Lassen (1958) reported a value of 32-8 ml./100 g and from the data given by Emery, Gowenlock, Riddel & Black (1959) an average of 35-1 ml./100 g whole kidney may be calculated. For cat kidneys, Pappenheimer & Kinter (1956) reported a figure of 31-0 ml./100 g. Values reported by Ulfendahl (1962) were smaller and ranged between 14-0 and 22-3 ml./100 g tissue for cat and rabbit kidneys under various diuretic conditions.…”

“…It has been suggested that a fraction of plasma albumin in the kidney is present in the interstitial space (Pappenheimer & Kinter, 1956;Lilienfield et al 1958;Swann, 1960). With histological techniques Pomerantz, Slotkoff & Lilienfield (1965) found albumin, marked with a fluorescent dye, in the renal interstitium, but this observation was not confirmed by Carone, Everett, Blondeel & Stolarczyk (1967).…”