Divergent mRNA transcription in the chloroplast psbB operon.

Kohchi, Takayuki; Yoshida, Takeshi; Komano, Tohru; Ohyama, Kanji

doi:10.1002/j.1460-2075.1988.tb02892.x

Cited by 62 publications

(52 citation statements)

References 44 publications

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“…This is in sharp contrast with pea (Pisum sativum) seedlings, with no psbN transcripts in the dark and a strong induction upon illumination (Kohchi et al, 1988).…”

Section: Introductioncontrasting

confidence: 64%

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PsbN Is Required for Assembly of the Photosystem II Reaction Center in Nicotiana tabacum

et al. 2014

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ORCID ID: 0000-0003-2973-9514 (J.M.)The chloroplast-encoded low molecular weight protein PsbN is annotated as a photosystem II (PSII) subunit. To elucidate the localization and function of PsbN, encoded on the opposite strand to the psbB gene cluster, we raised antibodies and inserted a resistance cassette into PsbN in both directions. Both homoplastomic tobacco (Nicotiana tabacum) mutants ΔpsbN-F and ΔpsbN-R show essentially the same PSII deficiencies. The mutants are extremely light sensitive and failed to recover from photoinhibition. Although synthesis of PSII proteins was not altered significantly, both mutants accumulated only ;25% of PSII proteins compared with the wild type. Assembly of PSII precomplexes occurred at normal rates, but heterodimeric PSII reaction centers (RCs) and higher order PSII assemblies were not formed efficiently in the mutants. The ΔpsbN-R mutant was complemented by allotopic expression of the PsbN gene fused to the sequence of a chloroplast transit peptide in the nuclear genome. PsbN represents a bitopic trans-membrane peptide localized in stroma lamellae with its highly conserved C terminus exposed to the stroma. Significant amounts of PsbN were already present in dark-grown seedling. Our data prove that PsbN is not a constituent subunit of PSII but is required for repair from photoinhibition and efficient assembly of the PSII RC.

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“…This is in sharp contrast with pea (Pisum sativum) seedlings, with no psbN transcripts in the dark and a strong induction upon illumination (Kohchi et al, 1988).…”

Section: Introductioncontrasting

confidence: 64%

“…The PsbN gene (ORF43) was discovered on the opposite strand to the plastidic psbB gene cluster between psbTc and psbH (Kohchi et al, 1988). It encodes a low molecular weight protein of 4.7 kD with a predicted single N-terminal transmembrane domain.…”

Section: Introductionmentioning

confidence: 99%

PsbN Is Required for Assembly of the Photosystem II Reaction Center in Nicotiana tabacum

et al. 2014

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“…Combining these results with our previous data [10, (psbN) have not yet been detected in plants. However, it is known that ORF43 is actively transcribed as monocistronic mRNA in liverwort and pea [12]. Since ORF34 as well as ORF43 is highly conserved between tobacco and liverwort ( fig.2), ORF34 may also be expressed.…”

Section: Discussionmentioning

confidence: 99%

N‐terminal sequencing of low‐molecular‐mass components in cyanobacterial photosystem II core complex Two components correspond to unidentified open reading frames of plant chloroplast DNA

1989

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We recently reported the presence of several low-molecular-mass protein components in the PS II O2-evolving core complex from the thermophilic cyanobacterium, Synechococcus vulcanus [(1989) FEBS Lett. 244, 391-396]. Here we have characterized the three components (4.1, 4.7, 5 kDa) of the same cyanobacterial core complex by N-terminal sequencing. There were two components in the 4.7 kDa region, both having a blocked N-terminus. One has a sequence highly homologous to open reading frame 34 of plant chloroplast DNA (tentatively designated psbM), while the other has a sequence partially homologous to open reading frame 43 of chloroplast DNA (designated psbN), although neither of the two gene products has yet been confirmed in chloroplasts. The cyanobacterial 4.1 kDa protein partially corresponds to the 4.1 kDa nuclear-encoded core component of higher plant PS II. The cyanobacterial 5 kDa component, however, shows a sequence that is unrelated to any other known proteins.

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“…Accumulation of partially processed RNAs leads to a complex pattern of approximately 20 different sized transcripts in leaf tissue (Barkan, 1988;Westhoff and Herrmann, 1988). Transcription also occurs in the opposite direction on the other strand in this region, producing ORF43 RNA from a sequence between psb6 and psbH (Kohchi et al, 1988). In leaf meristem and root tissues, the relative proportion of RNA from the psb6 operon as well as other chloroplast RNAs that do not undergo exon splicing is much greater than in mature leaf tissue (Barkan, 1989).…”

Section: Introductionmentioning

confidence: 99%

Chloroplast RNA Stability in Chlamydomonas: Rapid Degradation of psbB and psbC Transcripts in Two Nuclear Mutants.

1991

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Divergent mRNA transcription in the chloroplast psbB operon.

Cited by 62 publications

References 44 publications

PsbN Is Required for Assembly of the Photosystem II Reaction Center in Nicotiana tabacum

PsbN Is Required for Assembly of the Photosystem II Reaction Center in Nicotiana tabacum

N‐terminal sequencing of low‐molecular‐mass components in cyanobacterial photosystem II core complex Two components correspond to unidentified open reading frames of plant chloroplast DNA

Chloroplast RNA Stability in Chlamydomonas: Rapid Degradation of psbB and psbC Transcripts in Two Nuclear Mutants.

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