Dithiothreitol supplementation mitigates hepatic and renal injury in bile duct ligated mice: Potential application in the treatment of cholestasis-associated complications

Heidari, Reza; Ghanbarinejad, Vahid; Mohammadi, Hamidreza; Ahmadi, Asrin; Esfandiari, Athena; Azarpira, Negar; Niknahad, Hossein

doi:10.1016/j.biopha.2018.01.018

Cited by 44 publications

(39 citation statements)

References 51 publications

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“…For this purpose, TBA reactive substances were applied; the reaction mixture contained 500 μL of homogenized tissue (10% w/v in KCl, 1.15% w/v), 1 mL of TBA (0.375% w/v), and 3 mL of phosphoric acid (1% w/v, pH =2). After vigorously mixing the mixture and bain-marie heating for 45 minutes at 100°C, n-butanol (2 mL) was added following cooling of the sample 14,26,27. Then, the samples were centrifuged at 10,000 g for 5 minutes, followed by vigorous mixing.…”

Section: Methodsmentioning

confidence: 99%

<p>Glycine supplementation mitigates lead-induced renal injury in mice</p>

Shafiekhani¹,

Ommati²,

Azarpira³

et al. 2019

JEP

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Purpose Lead (Pb) is an environmental pollutant responsible for various organ damages including renal injury. It seems that OS and associated events are crucial mechanisms of lead-induced renal dysfunction. The current study aimed to explore the potential protective effects of glycine against renal injury caused by lead in mice. Materials and methods Mature male mice (n=32) were allocated into four groups. The following treatment regimens were the control (vehicle-treated); Pb-acetate (20 mg/kg/day, gavage); Pb-acetate + glycine (500 mg/kg/day, IP); and Pb-acetate + glycine (1,000 mg/kg/day, IP). Pb-acetate + glycine was administered for 14 consecutive days, Pb-acetate was given first and then glycine at least 6 hours later. On day 15, the subjects were anesthetized, and samples were collected. Serum biomarkers such as BUN and serum creatinine were monitored along with formation of reactive oxygen species, lipid peroxidation, kidney GSH level, and histopathological changes. Results Based on the results, BUN and serum creatinine levels significantly increased following exposure to lead. Glycine supplementation (500 and 1,000 mg/kg, IP) decreased BUN and creatinine serum levels ( P <0.001). Biomarkers of OS were also reduced in renal tissue following glycine therapy in Pb-exposed mice ( P <0.001). Histopathological changes were observed in mice treated with lead as tubular dilation, protein cast, vacuolization, and inflammation. In this regard, glycine inhibited histopathological alterations in kidney caused by lead exposure. Conclusion It was found that glycine treatment significantly mitigated Pb-induced renal injury most likely through alleviating OS and the associated deleterious outcomes on the kidney tissue.

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Section: Methodsmentioning

confidence: 99%

<p>Glycine supplementation mitigates lead-induced renal injury in mice</p>

Shafiekhani¹,

Ommati²,

Azarpira³

et al. 2019

JEP

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“…In order to further improve the stability of the self‐assemblies, DTT was used to cross‐link these self‐assemblies via click reactions between the thiols of DTT and the surface alkynyls on the self‐assemblies. DTT is a dithiol compound that effectively reduces the disulfide bonds of the glutathione molecule or other peptides and preserves the cellular redox environment . The morphologies and sizes of non‐cross‐linked and cross‐linked SHP self‐assemblies in aqueous solutions were investigated using transmission electron microscopy (TEM), dynamic light scattering (DLS)/static light scattering (SLS), and 1H NMR spectrum.…”

Section: Resultsmentioning

confidence: 99%

Size‐Stable Supramolecular Hyperbranched Polymer Vesicles for Redox‐Triggered Double‐Drug Release

Wang

Yuan

et al. 2018

Macro Chemistry & Physics

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Supramolecular polymer vesicles (SPVs) with stimuli‐responsive features are promising multifunctional nanocarriers; however, improving the stability and developing multiple‐drug‐loaded SPVs remain key issues in this field. In this work, cross‐linked supramolecular hyperbranched polymer vesicles (SHPVs) with redox‐responsiveness are first constructed based on an AB2‐type macromonomer‐synthesized SHP. The obtained cross‐linked SHPVs exhibit much better size stability than those of non‐cross‐linked branched self‐assemblies, and higher double‐drug‐loading capacity compared with linear supramolecular polymer self‐assemblies. Particularly, these cross‐linked SHPVs exhibit a redox‐triggered, controlled double‐drug release behavior upon the addition of H2O2.

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“…Dispase II is a collagenase type IV with a mild proteolytic activity that has been used for isolation of labile primary cells such as porcine urothelial cells, lymphatic and embryonic endothelial cells, stem cells and Schwan cells [33][34][35][36]. In addition, protease inhibitors (STI and BSA) is recommended to protected cells from non-speci c proteolytic activity [37] and antioxidant (DTT) preserved in a cellular redox environment [38]. Therefore, the compounds present in the digestion medium were helping the preservation of the viability and cellular architecture of GEC.…”

Section: Discussionmentioning

confidence: 99%

A Novel Methodology For Isolation and Primary Culture of Swine Gastric Epithelial Cells.

Bautista-Amorocho

Silva-Sayago

Goyeneche-Patino

et al. 2020

Preprint

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Background: Culture of primary epithelial cells has a great advantage over tumor-derived or immortal cells lines since functional phenotype and genetic makeup are preserved. Swine model has proved to be helpful and reliable as a surrogate model in human diseases. Several porcine cell lines have been established from a variety of tissues and shown to extensively contribute to the current understanding of several pathologies, including cancer. However, few protocols for the isolation and culture swine gastric epithelial cells with phenotype preservation have been described. Therefore, the objective of this research was to develop a new methodology for establishing a primary cell culture from the fundic gland area of the porcine stomach.Results: Enzymatic disaggregation of gastric tissue by using a combination of collagenase type I and dispase II, protease inhibitors (soybean trypsin inhibitor and bovine serum albumin), and antioxidants (Dithiothreitol) allowed the isolation of gastric epithelial cells from the fundic gland area with viability > 90% during the incubation period. Gastric epithelial cells cultured in RPMI 1640, DMEM HG, and DMEM/F12 media did not lead to cell adhesion, cluster formation and cell proliferation. By contrast, Williams’ medium supplemented with growth factors supports the confluence and proliferation of a pure epithelial cell monolayer after 10 days of incubation at 37oC in a 5% CO2 incubator. Mucin-producing cell phenotype of primary isolates was confirmed by PAS staining as well as the expression of MUC1 and MUC20 genes by RT-PCR and DNAc sequencing. Swine Gastric epithelial cells also showed origin-specific markers such as epithelial membrane antigen (EMA), cytokeratin cocktail (AE1/AE3) and cytokeratin 18 (CK-18) detected by immunohistochemistry and immunofluorescence, respectively. Conclusions: A new methodology was successfully established for the isolation of primary gastric epithelial cells from the fundic gland area in a swine model, based on a combination of tissue specific proteases, protease inhibitors, and antioxidants. The formulation of Williams’ medium with growth factors for epithelial cells supports the adherence and maintains the functional phenotype of primary cells, which was confirmed by mucin production and expression of typical epithelial markers in the long term.

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Dithiothreitol supplementation mitigates hepatic and renal injury in bile duct ligated mice: Potential application in the treatment of cholestasis-associated complications

Cited by 44 publications

References 51 publications

<p>Glycine supplementation mitigates lead-induced renal injury in mice</p>

<p>Glycine supplementation mitigates lead-induced renal injury in mice</p>

Size‐Stable Supramolecular Hyperbranched Polymer Vesicles for Redox‐Triggered Double‐Drug Release

A Novel Methodology For Isolation and Primary Culture of Swine Gastric Epithelial Cells.

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