Diterpenoids from the Roots of Euphorbia fischeriana

Abstract

From the dried roots of Euphorbia fischeriana, seven new diterpenoids, 3alpha,17-dihydroxy-ent-pimara-8(14),15-diene (1), 7beta,11beta,12beta-trihydroxy-ent-abieta-8(14),13(15)-dien-16,12-olide (2), 17-acetoxyjolkinolide B (3), 13beta-hydroxy-ent-abiet-8(14)-en-7-one (4), 12-deoxyphorbaldehyde-13-acetate (5) 12-deoxyphorbaldehyde-13-hexadecacetate (6), and 12-deoxyphorbol-13-(9Z)-octadecanoate-20-acetate (7), and two known compounds, 12-deoxyphorbol-13-decanoate (8) and prostratin (9), were isolated. The structures of the new compounds were elucidated on the basis of spectroscopic analysis. The structure of compound 1 was confirmed by single-crystal X-ray crystallography. Compounds 3 and 8 exhibited potent cytotoxic activity to Ramos B cells with IC50 values of 0.023 and 0.0051 microg/mL, respectively.

Ent-abietane diterpenoids and their probable biogenetic precursors from the roots of Euphorbia fischeriana

“…By the comparison of spectroscopic data with literature, the known compounds isolated from the roots of E. scheriana were identied as 11b-hydroxy-8,14-epoxy-ent-abieta-13(15)-en-16,12olide (6), 12 8b,14b-epoxy-13,15-abiatene-16,12a-olide (7), 14 jolkinolide B (8), 15 17-hydroxyjolkinolide B (9), 15 jolkinolide A (10), 15 17-hydroxyjolkinolide A (11), 16 yuexiandajisu D (12), 17 ent-11b-hydroxyabieta-8 (14),13(15)-dien-16,12b-olide (13), 16 13bhydroxy-ent-abiet-8(14)-en-7-one (14), 18 respectively.…”

Two new diterpenes from the seeds ofCaesalpinia minaxHance

“…The NMR data suggested that compound 2 was a pimaran derivative [9]. In its HMBC spectrum (Figure 2), the correlation between H-2 and the carbonyl at d C 170.3 was observed, which indicted that this acetoxyl group was at C-2 position, meanwhile, the correlation between H-7 (d H 3.40) and the carbon at d C 26.9 (C-14) suggested that a hydroxyl group was located at C-7.…”

De novo assembly of Euphorbia fischeriana root transcriptome identifies prostratin pathway related genes

“…To obtain E. fischeriana transcriptome expression profiles in roots, and identify candidate genes upstream of prostratin synthesis, where traces of prostratin has been previously reported [1], Illumina technology was used to sequence an E. fischeriana library of transcripts expressed in roots generating more than 17.5 million pair-end short reads encoding 1.3 billion bases (Table 1). We initially evaluated the base quality of the sequenced reads (Additional file 1) and trimmed poor quality bases as well as removed poor quality reads (Additional file 2).…”

“…The dried plant roots of E. fischeriana and other Euphorbia species are called "Lang-Du", which means extremely toxic in Chinese. The roots have often been used in traditional Chinese medicine to treat a wide range of ailments, such as edema, ascites, and cancer [1]. Previous studies of the plant have revealed it mainly contains diterpenoids, triterpenoids and steroids [2].…”