“…To check for the presence of TmTHP in inclusion bodies, the pellet was resuspended in 8 ml of 8 M guanidine-HCl, 100 mM Tris-HCl (pH 8.5), 1 mM EDTA, 12 mM 2-mercaptoethanol and gently mixed at room temperature (22°C) for 1 h. This suspension was added dropwise to 160 ml of ice-cold refolding buffer, 50 mM K 2 HPO 4 , 1 mM EDTA, 2 mM 2-mercaptoethanol, and 100 mM NaCl (pH 10.7), and left to stir overnight at 15°C. The solution was dialyzed over 2 days against 10 mM Tris-HCl, 1 mM EDTA, and 100 mM NaCl, pH 8.0 (12), and left to oxidize for up to 1 month.…”