Disubstituted dialkoxysilane precursors in binary and ternary sol–gel systems for lipase immobilization

Immobilization of lipases from Rhizomucor miehei and Thermomyces lanuginosus by adsorption on variously grafted silica gels

“…Our results have shown that the optimal method of enzyme immobilization depended both on the nature of the substrate and the reaction conditions [13], [14]]. This study indicated that using a broad selection of variously grafted silica gels for the immobilization of RmL and TlL provided a selection of biocatalysts with a wide range of activity and selectivity when applied for the kinetic resolution of racemic 1-phenylethanol rac-1.…”

“…Among the many available immobilization methods, including adsorption, covalent attachment to solid supports and entrapment within polymers [8][9][10][11], hydrophobic adsorption onto suitable carriers was found to be an efficient way not only for immobilization but also for the separation of lipases [12]. Lipases immobilized by various procedures proved to be useful both in batch mode and in continuous-flow biotransformations [13][14][15][16].…”

From Synthetic Chemistry and Stereoselective Biotransformations to Enzyme Biochemistry – The Bioorganic Chemistry Group at the Budapest University of Technology and Economics

“…The sol-gel encapsulated enzymes usually retain their selectivity whereas their heat stability or specific activity may be significantly improved. BCG researchers investigated the lipase immobilization by entrapment in sol-gel matrices to study the effect of enzymes, supports, enzyme/ support ratios, silane precursor composition and bioimprinting effect of additives [43][44][45][46][47]. In addition to test the resulting solgel lipases in kinetic resolution of 1-phenylethanol 1j using batch mode [43][44][45][46][47], the reactions were studied in continuousflow systems as well [41].…”

“…Disubstituted dialkoxysilane precursors such as dimethyldiethoxysilane (DMDEOS), methyl(phenyl)diethoxysilane (MPDEOS) and diphenyldiethoxysilane (DPDEOS) were used as precursor components in binary and ternary sol-gel systems for immobilization of lipase from Pseudomonas fluorescens (Lipase AK) [47]. Tests with kinetic resolutions of 1-phenylethanol 1j, 1-phenylpropan-2-ol 1k and 4-phenylbutan-2-ol 1l indicated the catalytic properties and the operational stability of the DMDEOS-containing preparations as the best.…”

Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases

“…The methods based on physical interactions are gentle, but simple adsorption allows detachment of the enzyme from the carrier in recycling and reduces the cost-effectiveness of such biocatalysts [13,14]. Embedding of enzymes in polymeric matrix-for instance, by the sol-gel methodcan enhance their thermal and mechanic stability [14,15], but their activity is often reduced by their restricted accessibility. When the polymer-embedded enzyme system is prepared by evaporating the solvent from a common solution, emulsion or suspension [16], the heat-sensitive enzymes tolerate only extremely gentle way of solvent evaporation.…”