Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases

Abstract

Electrospinning was applied to create easy-tohandle and high-surface-area membranes from continuous nanofibers of polyvinyl alcohol (PVA) or polylactic acid (PLA). Lipase PS from Burkholderia cepacia and Lipase B from Candida antarctica (CaLB) could be immobilized effectively by adsorption onto the fibrous material as well as by entrapment within the electrospun nanofibers. The biocatalytic performance of the resulting membrane biocatalysts was evaluated in the kinetic resolution of racemic 1-phenylethanol (rac-1) and 1-phenylethyl acetate (rac-2). Fine dispersion of the enzymes in the polymer matrix and large surface area of the nanofibers resulted in an enormous increase in the activity of the membrane biocatalyst compared to the non-immobilized crude powder forms of the lipases. PLA as fiber-forming polymer for lipase immobilization performed better than PVA in all aspects. Recycling studies with the various forms of electrospun membrane biocatalysts in ten cycles of the acylation and hydrolysis reactions indicated excellent stability of this forms of immobilized lipases. PLA-entrapped lipases could preserve lipase activity and enantiomer selectivity much better than the PVA-entrapped forms. The electrospun membrane forms of CaLB showed high mechanical stability in the repeated acylations and hydrolyses than commercial forms of CaLB immobilized on polyacrylamide beads (Novozyme 435 and IMMCALB-T2-150).

Real-Time Analysis of Tenofovir Release Kinetics Using Quantitative Phosphorus (31P) Nuclear Magnetic Resonance Spectroscopy

“…PLA is biodegradable aliphatic polyester, and has been approved by the FDA for human clinical applications. PLA is a good fiber-forming polymer 30, 31 . Moreover, PLA helps crosslink and solidify the shell by forming hydrogen bound with chitosan 32 .…”

Poly(2‐ethyloxazoline) as matrix for highly active electrospun enzymes in organic solvents

“…Direct electrospinning of Burkholderia cepacia lipase (BCL) with polycaprolactone (PCL) in a mixture of dimethylformamide/chloroform showed fourfold activation in a transesterification reaction (Song et al, ). Sóti et al () activated CaLB by a factor of 22 and BCL by a factor of 47 by entrapping it in electrospun PLA fibers.…”

“…Altogether, these results are very promising particularly when directly electrospinning from polymer enzyme solutions. More recently, Sóti et al () have reported on electrospinning of different lipases looking at poly lactic acid (PLA) as an alternative to PVA in different lipase catalyzed reactions. They found in all cases that PLA is the more active support.…”

Immobilization engineering – How to design advanced sol–gel systems for biocatalysis?

“…4: residual activities were 20% for CV-T2-150 and 64% for N 435 after 10 cycles). 34 Mie scattering of the sol-gel entrapped CaLBs after ultrasonicationcompared to their initial statesproved that these CaLB biocatalysts comprised quite stable particles (with 10-100 µm particle size range, see section 7 and Fig. S13, in the ESI †).…”