Distributions of PCNA and Cas-3 in rat uterus during early pregnancy.

Öner, Hakan; Öner, Jale; Demir, Ramazan

doi:10.2478/v10042-008-0088-2

Cited by 10 publications

(7 citation statements)

References 30 publications

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“…Furthermore, several studies have suggested that endometrial cell death is regulated by Caspase-3 during the menstrual or estrous cycle [113] and during early pregnancy [114]. In this study, we found that the miR-182 mimics increased the Caspase-3 protein levels at 48 h, and miR-182 inhibitors decreased the levels of Caspase-3.…”

Section: Discussionsupporting

confidence: 62%

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

Zhang

Liu

et al. 2017

PLoS ONE

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Increasing evidence has shown that miRNAs play important roles in endometrium development during the menstrual cycle in humans and many other animals. Our previous data indicated that miR-182 levels increase 15.55-fold and pleiotrophin (PTN) levels decrease 20.97-fold in the receptive endometrium (RE, D15) compared with the pre-receptive endometrium (PE, D5) in dairy goats. The present study shows that miR-182 is widely expressed in different tissues of dairy goats and that its expression levels are regulated by E2 and P4 in endometrial epithelium cells (EECs). We confirmed that PTN is a target of miR-182 and that miR-182 regulates the protein levels of AKT, Bcl-2, FAS, MAPK, Caspase-3 and SP1 in EECs. Furthermore, miR-182 up-regulates or maintains the expression levels of osteopontin (OPN), cyclooxygenase-2 (COX-2) and prolactin receptor (PRLR) in EECs, suggesting that miR-182 is an important regulatory factor in the construction of endometrial receptivity in dairy goats. In conclusion, miR-182 participates in the development of endometrial receptivity by down-regulating PTN and affecting the expression of select apoptosis-related genes and increasing or maintaining the expression levels of OPN, COX-2 and PRLR in the EECs of dairy goats.

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Section: Discussionsupporting

confidence: 62%

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

Zhang

Liu

et al. 2017

PLoS ONE

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“…Cas-3/PCNA balance in the luminal and glandular epithelium shifts death inducers after E3, and blastocyst implantation induces uterine luminal epithelial cell death and stromal cell proliferation around the embryo in the uterus (Oner et al, 2010). This present study considered the ratio of MOD PCNA /MOD TUNEL as indicative of the state of endometrial cell development.…”

Section: Endometrial Proliferationmentioning

confidence: 98%

Restraint stress delays endometrial adaptive remodeling during mouse embryo implantation

Liu¹,

Dong²,

Wang³

et al. 2015

Stress

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We showed previously in mice that restraint stress reduces the number of embryo implantation sites in the endometrium. Here we hypothesized that the uterine microenvironment is altered by restraint stress and consequently is sub-optimal for embryo implantation. On embryonic day 1 (E1), 60 of 154 pregnant CD1 mice underwent restraint stress (4h), repeated daily to E3, E5 or E7 (n = 10 mice per group). Restraint stress decreased food intake and suppressed body weight gain on E3, E5 and E7. Restraint stress decreased the actual and relative weight (percent body weight) of uterus and ovary on E5 (by 14.9%, P = 0.03; 16.1%, P = 0.004) and E7 (by 16.8%, P = 0.03; 20.0%, P = 0.01). Morphologically, restraint stress decreased relative endometrial area (by 8.94-18.8%, P = 0.003-0.021) and uterine gland area (by 30.6%, P < 0.01 on E3 and 44.5%, P < 0.01 on E5). Immunohistochemistry showed that restraint stress decreased microvessel density (by 12.9-70.5%, P < 0.01) and vascular endothelial growth factor expression (by 14.6-45.9%, P = 0.007-0.02). Restraint stress decreased by 32.4-39.8% (P = 0.002-0.01) the mean optical density ratio for proliferating cell nuclear antigen/terminal deoxynucleotidyl transferase dUTP nick end labeling. Methyl thiazolyl tetrazolium assay showed a dosedependent decrease in proliferative activity of endometrial stromal cells (from 52 of 154 pregnant E5 control mice) incubated with H 2 O 2 (100-1000 μM) in vitro. These findings supported the hypothesis that restraint stress negatively influences endometrial adaptive remodeling via an oxidative stress pathway, which resulted in fewer implantation sites. Stress Downloaded from informahealthcare.com by Nanyang Technological University on 08/26/15 For personal use only. Stress Downloaded from informahealthcare.com by Nanyang Technological University on 08/26/15 For personal use only. Stress Downloaded from informahealthcare.com by Nanyang Technological University on 08/26/15 For personal use only. Stress Downloaded from informahealthcare.com by Nanyang Technological University on 08/26/15 For personal use only. Stress Downloaded from informahealthcare.com by Nanyang Technological University on 08/26/15 For personal use only.

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“…The cell cycle regulators are critical for controlling the stromal cell proliferation and differentiation during the implantation stage [ 50 ]. PCNA (proliferating cell nuclear antigen) is a cell cycle promoting gene which was observed primarily in uterine luminal epithelium and glandular epithelium at pre-implantation stage and its expression level was increased gradually in stromal cells and myometrium with progressing gestation [ 51 , 52 , 53 ]. This increased expression of cell cycle-related genes at the time of Meishan embryo implantation suggested that the cell proliferation and differentiation in Meishan endometrium were associated with the embryo attachment.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Revealed the Embryo-Induced Gene Expression Patterns in the Endometrium from Meishan and Yorkshire Pigs

Huang

Liu

et al. 2015

IJMS

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The expression patterns in Meishan- and Yorkshire-derived endometrium during early (gestational day 15) and mid-gestation (gestational days 26 and 50) were investigated, respectively. Totally, 689 and 1649 annotated genes were identified to be differentially expressed in Meishan and Yorkshire endometrium during the three gestational stages, respectively. Hierarchical clustering analysis identified that, of the annotated differentially expressed genes (DEGs), 73 DEGs were unique to Meishan endometrium, 536 DEGs were unique to Yorkshire endometrium, and 228 DEGs were common in Meishan and Yorkshire endometriums. Subsequently, DEGs in each of the three types of expression patterns were grouped into four distinct categories according to the similarities in their temporal expression patterns. The expression patterns identified from the microarray analysis were validated by quantitative RT-PCR. The functional enrichment analysis revealed that the common DEGs were enriched in pathways of steroid metabolic process and regulation of retinoic acid receptor signaling. These unique DEGs in Meishan endometrium were involved in cell cycle and adherens junction. The DEGs unique to Yorkshire endometrium were associated with regulation of Rho protein signal transduction, maternal placenta development and cell proliferation. This study revealed the different gene expression patterns or pathways related to the endometrium remodeling in Meishan and Yorkshire pigs, respectively. These unique DEGs in either Meishan or Yorkshire endometriums may contribute to the divergence of the endometrium environment in the two pig breeds.

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Distributions of PCNA and Cas-3 in rat uterus during early pregnancy.

Cited by 10 publications

References 30 publications

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

Restraint stress delays endometrial adaptive remodeling during mouse embryo implantation

Transcriptome Analysis Revealed the Embryo-Induced Gene Expression Patterns in the Endometrium from Meishan and Yorkshire Pigs

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