Distributions of high-density lipoprotein particle components in human follicular fluid and sera and their associations with embryo morphology parameters during IVF

Browne, Richard W.; Shelly, W.B.; Bloom, Michael S.; Ocque, Andrew J.; Sandler, J.R.; Huddleston, Heather G.; Fujimoto, Victor Y.

doi:10.1093/humrep/den183

Cited by 53 publications

(82 citation statements)

References 67 publications

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“…11,38 To our knowledge, the first study investigated PON activity in FF was conducted by Browne et al and they have found that PON1-arylesterase activity in FF was a positive predictor for Day 3 embryo cell number. 10 Contrary to that study, we found similar FF levels of PON1 and arylesterase activity in women with PCOS and in controls. It is thought that proven conditions including increased oxidative stress in women with PCOS is not the cause of the infertility in those women, however; in our study, differently from other studies, the oxidative molecules have been investigated in follicle fluid representing the biological window of ovary.…”

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confidence: 99%

“…9 The effect of oxidative stress on etiology of PCOS and effect of PON1 in women with PCOS are still debate; there are a few studies with conflicting results about relationship between PON1 and PCOS. [10][11][12][13] Therefore we aimed to compare the follicular fluid level of PON and ARES activity in women with PCOS and those with Male factor infertility (MFI) who underwent assisted reproductive technique (ART) using intra-cytoplasmic sperm injection (ICSI).…”

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Comparison of the Paraoxonase and Arylesterase Activities in Follicle Fluid of Females with PCOS in ICSI Cycle

Özdeğirmenci¹,

Dilbaz²,

Tohma³

et al. 2017

TJRMS

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A AB BS S T TR RA AC CT TO Ob bj je ec ct ti iv ve e: : The aim of our study was to compare the Paraoxonase (PON1) and arylesterase (ARES) activity in follicle fluids of infertile females with polycystic ovary syndrome (PCOS). M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : A total of 93 women who underwent assisted reproductive technologies (ART) using intra-cytoplasmic sperm injection (ICSI) were enrolled in this prospective study, of whom 46, with PCOS, comprised the study group, the remaining 47 women with MFI formed the control group. Women age, body mass index, (BMI) basal hormone levels, total follicle count, total oxidant status (TOS), total antioxidant status (TAS), PON1 and ARES activity, oxidative stress index (OSI), simulated paraoxonase (SPON) were compared between groups. R Re es su ul lt ts s: : There were no significant differences for average age, BMI, Day3-FSH, Day3-LH, Day3-E2 and total follicle count among groups. Also there were no statistically significant differences among groups in terms of TOS, TAS, PON1, SPON, ARES and OSI (p>0.05). C Co on nc cl lu us si io on n: : TOS, TAS, OSI, SPON, PON1 and ARES enzyme activities in follicular fluid of infertile females with PCOS are similar in those with male factor infertility.K Ke ey y W Wo or rd ds s: : Paraoxonase; arylesterase; follicular fluid; polycystic ovary syndrome; infertility Ö ÖZ ZE ET T A Am ma aç ç: : Çalışmamızdaki amacımız Poikistik Over Sendromu (PKOS) olan infertil kadınların folikül sıvılarındaki Paraksonaz (PON1) ve arilesteraz (ARES) aktivitelerinin karşılaştırılması. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Yardımcı üreme tekniği (YÜT) ile intra-sitoplazmik sperm enjeskiyonu (ICSI) uygulanan toplam 93 kadın, bu prospektif çalışmaya dahil edildi, bu kadınlardan PKOS'lu olan 46' sı çalışma grubunu ve sadece erkek faktörü bulunan geri kalan 47'si kontrol grubunu oluşturdu. Kadınalrın yaşı, vücut kitle indeksi (VKİ), bazal hormon seviyeleri, toplam folikül sayıları, total oksidan status (TOS), total antioksidan status (TAS), PON1 ve ARES aktiviteleri, oksidatif stres indeksi (OSİ), simüle edilmiş paraksonaz (SPON) gruplar arasında karşılaştırıldı. B Bu ul lg gu ul la ar r: : Gruplar arasında ortalama yaş, VKİ, 3. Gün FSH-LH-Estadiol seviyeleri ve total folikül sayıları açısından anlamlı bir fark yoktu. Ayrıca TOS, TAS, PON1, SPON, ARES ve OSI seviyeleri arasında anlamlı bir farklılık yoktu (p>0.05). S So on nu uç ç: : Folikül sıvısı içindeki TOS, TAS, OSI, SPON, PON1 ve ARES'in enzim aktiviteleri PKOS'lu kadınlarda ve erkek faktörü nedeniyle YÜT uygulanan kadınlarda benzerdi. Ya zış ma Ad re si/Cor res pon den ce:

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confidence: 99%

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confidence: 99%

Comparison of the Paraoxonase and Arylesterase Activities in Follicle Fluid of Females with PCOS in ICSI Cycle

Özdeğirmenci¹,

Dilbaz²,

Tohma³

et al. 2017

TJRMS

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“…Thus, the structure, size, and antioxidant activity of HDL are dynamic. We previously conducted a pilot study of HDL particle components, including lipids, ApoA-1, and paraoxonase 1 (PON1) activities measured in FF and human serum, and their associations with embryo quality on 60 women undergoing in vitro fertilization (IVF) [21]. In that study, we identified FF ApoA-1 and HDL-cholesterol (HDL-C) as predictors of embryo fragmentation, suggesting protective associations for embryo quality.…”

Section: Introductionmentioning

confidence: 99%

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

Kim

Bloom

Browne

et al. 2016

J Assist Reprod Genet

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Purpose Follicular redox balance is likely to be important for embryo quality during in vitro fertilization (IVF), and the anti-oxidative high desity lipoprotein (HDL) particle is the sole lipoprotein measured in follicular fluid (FF). Therefore, we investigated FF HDL particle components as predictors of embryo quality during IVF. Methods Two research follicles collected from each participant were individually tracked, and 103 women having at least one developed embryo were included in the analysis. Concentrations of 15 non-cholesterol HDL particle components and 26 HDL-cholesterol (HDL-C) particle size subfractions were determined. Embryo quality was assessed for embryo cell number, embryo fragmentation, and embryo symmetry. Multivariable Poisson regression with a sandwich variance estimator was used to evaluate associations between HDL particle components and embryo quality, adjusted for covariates. Results Higher γ-tocopherol concentration was associated with less embryo fragmentation (relative risk [RR] = 4.43; 95 % confidence interval [CI] 1.78, 11.06), and higher apolipoprotein A-1 concentration was associated with full embryo symmetry (RR = 3.92; 95 % CI 1.56, 9.90). Higher concentrations of HDL-C subfractions in the large and medium particle size ranges were associated with poorer embryo quality. Conclusions FF HDL lipophilic micronutrients and protein components, as well as HDL-C particle size, may be important predictors of embryo quality during IVF.

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“…Arylesterase and paraoxonase activities were also analyzed using the Cobas Fara II as described elsewhere [22]. FF HDL fractions were prepared by selective precipitation to remove trace amounts of apolipoprotein B containing low density lipoproteins [17].…”

Section: Analytic Methodsmentioning

confidence: 99%

“…Given its potent antioxidant activity [12][13][14], and critical role in delivering cholesterol for steroid hormone synthesis [15,16], HDL has strong potential as a biomarker of the follicular microenvironment. Studies also identified HDL particle components measured in FF, including cholesterol, ApoA-1 [17], γ-tocopherol, and β-cryptoxanthin [18], as important predictors of embryo quality during in vitro fertilization (IVF). Higher FF apolipoprotein A-1 (ApoA-1) levels were recently associated with poorer embryo quality per oocyte, in women undergoing IVF [19].…”

Section: Introductionmentioning

confidence: 99%

Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles

Kim

Bloom

Fujimoto

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of this study was to examine the biological variability of follicular fluid (FF) high density lipoprotein (HDL) particle components measured in ipsilateral ovarian follicles. Methods We collected FF from two ipsilateral follicles among six women undergoing in vitro fertilization (IVF). We measured concentrations of 19 FF HDL particle components, including HDL cholesterol, free cholesterol, four cholesteryl esters, phospholipids, triglycerides, paraoxonase and arylesterase activities, apolipoproteins A-1 and A-2 (ApoA-1 and ApoA-2), and seven lipophilic micronutrients, by automated analysis and with high-performance liquid chromatography. We assessed biological variability using two-stage nested analysis of variance and compared values with those previously published for contralateral follicles.Results For most FF HDL analytes, there was little variability between follicles relative to the variability between women (i.e., %σ 2 F :%σ 2 B <0.5). Intraclass correlation coefficients were >0.80 for HDL cholesterol (0.82), phospholipids (0.89), paraoxonase (0.96), and arylesterase (0.91) activities, ApoA-1 (0.89), and ApoA-2 (0.90), and single specimen collections were required to estimate the subject-specific mean, demonstrating sufficient reliability for use as biomarkers of the follicular microenvironment in epidemiologic and clinical studies. Conclusions These preliminary results raise the possibility for tighter regulation of HDL in follicles within the same ovary vs. between ovaries. Thus, collection of a single FF specimen may be sufficient to estimate HDL particle components concentrations within a single ovary. However, our results should be interpreted with caution as the analysis was based on a small sample.

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Distributions of high-density lipoprotein particle components in human follicular fluid and sera and their associations with embryo morphology parameters during IVF

Cited by 53 publications

References 67 publications

Comparison of the Paraoxonase and Arylesterase Activities in Follicle Fluid of Females with PCOS in ICSI Cycle

Comparison of the Paraoxonase and Arylesterase Activities in Follicle Fluid of Females with PCOS in ICSI Cycle

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

Variability in follicular fluid high density lipoprotein particle components measured in ipsilateral follicles

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