Distribution of β-Lactamase Genes Among Carbapenem-Resistant<i>Klebsiella pneumoniae</i>Strains Isolated From Patients in Turkey

Iraz, Mustafa; Düzgün, Azer Özad; Sandallı, Cemal; Doymaz, Mehmet Ziya; Akkoyunlu, Yasemin; Saral, A.; Peleg, Anton Y.; Özgümüş, Osman Birol; Beriş, Fatih Şaban; Karaoglu, Hakan; Çiçek, Ayşegül Çopur

doi:10.3343/alm.2015.35.6.595

Cited by 44 publications

(41 citation statements)

References 28 publications

(37 reference statements)

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“…In the present study, KPC, IMP, and VIM were not determined from any of the isolates. Similar to the current study data, 2 recent studies performed on carbapenem-resistant K. pneumoniae clinical isolates from different hospitals of Turkey, no positive results for KPC, IMP, and VIM enzymes were reported (40,41). In a multicentral surveillance study, the prevalence of VIM, IMP, and KPC among 136 carbapenemresistant E. coli and K. pneumoniae isolates was reported as 2.8%, 1.4%, and 0%, respectively (33).…”

Section: Discussionsupporting

confidence: 74%

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Çelikbilek¹,

Ünaldı²,

Kırca³

et al. 2017

Jundishapur J Microbiol

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Background: Carbapenem-resistant Klebsiella pneumoniae isolates can spread among the hospitalized patients and result in serious infections and increase mortality. Molecular studies provide useful information about resistance mechanisms and crosstransmission among the resistant isolates. Objectives: The current study aimed at investigating phenotypic and molecular characteristics of the carbapenem resistance and clonal relationship among the K. pneumoniae isolates recovered from infection sites and rectal swabs of the patients hospitalized in a tertiary hospital. Methods: Resistance to carbapenems was investigated by disc-diffusion and E-test methods. Modified Hodge test (MHT) and ethylenediaminetetraacetic acid (EDTA)-combine disc (ECD) methods were performed to determine carbapenemases. Carbapenemase-encoding genes including blaOXA-48, blaNDM-1, blaKPC, blaIMP, and blaVIM were investigated by multiplex polymerase chain reaction (PCR). Clonal relationship among the isolates was determined by the pulsed-field gel electrophoresis (PFGE). Results: Carbapenem resistance was observed in 93 (5.8%) of the 1605 K. pneumoniae isolates. Majority (n = 66, 71%) of the resistant isolates were recovered from the patients in intensive care units (ICUs). Almost all carbapenem-resistant K. pneumoniae isolates (n = 91, 97.8%) were positive with MHT. Only 6 (6.5%) of the resistant isolates were positive with ECD method. The blaOXA-48 and blaNDM-1 were found in 90.3% (n = 84) and 6.5% (n = 6) of the resistant isolates, respectively. The pulsed-field gel electrophoresis yielded

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Section: Discussionsupporting

confidence: 74%

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Çelikbilek¹,

Ünaldı²,

Kırca³

et al. 2017

Jundishapur J Microbiol

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“…Udomsantisuk et al 53 reported that the frequency of the bla VEB gene was 30% among β-lactamase-positive K. pneumoniae strains; however, in the current study, the frequncy of bla VEB gene was 12%. Iraz et al 54 reported that 86% of the carbapenem-resistant K. pneumoniae strains carried the bla OXA gene; however, Charrouf et al 55 found that only 6% of their isolates carried this gene. Our results did not match either of these studies; in our study, the prevalence of this gene was 28%.…”

mentioning

confidence: 99%

Molecular detection of β-lactamase and integron genes in clinical strains of Klebsiella pneumoniae by multiplex polymerase chain reaction

Sedighi

Halajzadeh

Ramazanzadeh

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction:Infections caused by β-lactamase-producing gram-negative bacteria, such as Klebsiella pneumoniae, are increasing globally with high morbidity and mortality. The aim of the current study was to determine antimicrobial susceptibility patterns and the prevalence of antibiotic resistance genes (β-lactamase and integron genes) using multiplex PCR. Methods: One-hundred K. pneumoniae isolates were collected from different clinical samples. Antibiotic susceptibility testing was performed with thirteen different antibiotics. Multiplex-PCR was used to detect β-lactamase (bla TEM , bla CTX-M, bla SHV, bla VEB , bla PER , bla GES , bla VIM, bla IMP, bla OXA , and bla KPC ) and integron genes (int I, int II, and int III). Results: The highest and lowest rate of resistance was exhibited against amikacin (93%) and imipenem (8%), respectively. The frequency of β-lactamase-positive K. pneumoniae was 37%, and the prevalence of the bla TEM , bla CTX-M, bla SHV, bla VEB , bla PER , bla GES , bla VIM, bla IMP, bla OXA , and bla KPC genes was 38%, 24%, 19%, 12%, 6%, 11%, 33%, 0%, 28%, and 23%, respectively. Of the 100 isolates, eight (8%) were positive for class I integrons; however, class II and III integrons were not detected in any of the strains. Conclusions: These results indicate co-carriage of a number of β-lactamase genes and antibiotic resistance integrons on the same plasmids harboring multi-drug resistance genes. It seems that these properties help to decrease treatment complications due to resistant bacterial infections by rapid detection, infection-control programs and prevention of transmission of drug resistance.

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“…Yunanistan'da 2007 yılında yapılan bir çalışmada, çoklu ilaç direnci bulunan patojenlerle enfeksiyon nedeniyle kolistin kullanılan yoğun bakım hastalarında, kolistine dirençli K. pneumoniae ile bronşiyal ve bağırsak kolonizasyon oranı %37 olarak saptanmıştır (20). Karbapenem dirençli K. pneumonia suşlarında da İtalya'da %43, Türkiye'de %2,7 kolistin direnci bildirilmiştir (21,22). Yine İtalya'dan bildirilen bir çalışmada, karbapenemaz üreten K. pneumonia suşlarında kolistin direncinin 2010-2013 yılları arasında %11'den %27'ye yükseldiği belirtilmiştir (23).…”

Section: Methodsunclassified

Extended spectrum beta-lactamase production and antibiotic resistance of Escherichia coli and Klebsiella spp. isolated from hospitalized patients: data of 2011-2015

Pekintürk¹,

Akgunes²

2017

Turk Hij Den Biyol Derg

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Distribution of β-Lactamase Genes Among Carbapenem-ResistantKlebsiella pneumoniaeStrains Isolated From Patients in Turkey

Cited by 44 publications

References 28 publications

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Molecular detection of β-lactamase and integron genes in clinical strains of Klebsiella pneumoniae by multiplex polymerase chain reaction

Extended spectrum beta-lactamase production and antibiotic resistance of Escherichia coli and Klebsiella spp. isolated from hospitalized patients: data of 2011-2015

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