Distribution of Yellow Curly Leaf Disease in Chili Plantations in Southeast Sulawesi and Identification of the Causes

Taufik, Muhammad; Hs, Gusnawaty; Syair, Syair; Mallarangeng, Rahayu; Khaeruni, Andi; Botek, Muhammad; Hartono, Slamet; Aidawati, Noor; Hidayat, Purnama

doi:10.14692/jfi.19.3.89-98

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…Gejala kuning keriting yang sering ditemukan pada tanaman cabai disebabkan oleh infeksi virus dari genus Begomovirus. Tanaman yang terinfeksi oleh virus ini mengalami perubahan pola warna daun menjadi mosaik kuning dan morfologi bentuk daun menjadi abnormal (menangkup, keriting, atau mengerdil) (Selangga et al 2019;Taufik et al 2023c;Taufik et al 2023d).…”

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Morphometric Analysis of Chili Leaves with Yellow Curly Symptom Using Digital Image Processing Approach and Data Mining Algorithm

Hasan,

Taufik,

Bande

et al. 2023

J Fitopatol Indones

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Gejala kuning keriting pada daun cabai umumnya disebabkan oleh infeksi Begomovirus. Daun tanaman terinfeksi tidak hanya mengalami perubahan warna sebagai indikator rusaknya klorofil tetapi juga mengalami perubahan morfologi bentuk. Penelitian ini bertujuan menguantifikasi gejala infeksi Begomovirus berdasarkan perubahan morfologi bentuk daun menggunakan pengolahan citra digital dan algoritma data mining yang akan memudahkan dalam pemantauan dan analisis perkembangan penyakit tanaman. Total 33 citra daun cabai rawit bergejala kuning keriting maupun tidak bergejala menjadi dataset penelitian ini. Citra daun cabai tersebut diolah dan diekstrak karakteristik bentuknya berupa circularity, aspect ratio, roundness, dan solidity menggunakan aplikasi Fiji-ImageJ. Selanjutnya dilakukan uji beda (uji-t), pengelompokan citra menggunakan algoritma Simple K-Means, dan evaluasi ketepatan hasil pengelompokan berdasarkan indeks ARI dan NMI. Hasil penelitian menunjukkan bahwa secara umum ada perbedaan bentuk yang nyata antara daun bergejala dengan daun tidak bergejala. Daun cabai rawit bergejala kuning keriting memiliki rata-rata nilai aspect ratio dan solidity yang lebih kecil dibandingkan daun cabai tidak bergejala, sebaliknya memiliki rata-rata nilai circularity dan roundness yang lebih besar dibandingkan daun cabai tidak bergejala. Evaluasi ketepatan pengelompokan sampel daun cabai rawit bergejala maupun tidak bergejala berdasarkan indeks ARI dan NMI menghasilkan nilai terbaik untuk pengelompokkan ke dalam dua kelompok.

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Morphometric Analysis of Chili Leaves with Yellow Curly Symptom Using Digital Image Processing Approach and Data Mining Algorithm

Hasan,

Taufik,

Bande

et al. 2023

J Fitopatol Indones

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Evaluation of three different DNA extraction methods for the detection of Pepper yellow leaf curl virus (PepYLCV) by Polymerase Chain Reaction

Paradisa,

Hidayat,

Saputra

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Pepper yellow leaf curl virus (PepYLCV) from the genus Begomovirus infecting Chili pepper plant can cause significant yield losses. PepYLCV infection can lead the development of mosaic yellow, mottled pattern and yellowing leaves, which can be challenging in disease management and control. Developing effective detection methods for PepYLCV is a critical disease management and crop loss mitigation. The Begomovirus viruses have extremely low viral concentrations and are limited to the phloem and vascular system. DNA extraction is an important step in PepYLCV detection. This study aimed to evaluate the efficiency of three DNA extraction methods: phenol-based extraction, CTAB-based extraction, and the GeneJET Plant Genomic DNA Purification Kit (Thermo Scientific™). These methods were evaluated for their performance to identified the presence of PepYLCV DNA and provide accurate results for further analyses. DNA samples were extracted from two varieties including Red Habanero and F8 012328-6-2-1-1-3-1, and were subsequently amplified using Krusty Homer’s primers. The results showed that all three extraction methods possess the capability to identify PepYLCV. Based on the results, DNA extraction employing the CTAB-based method yields DNA with higher concentration and purity levels. Additionally, this method proves to be cost-effective and proficient in generating higher DNA concentrations.

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Initiatives and Prospects for Sustainable Agricultural Production in Karangasem Regency, Bali, Indonesia

Hirakawa,

Masuyama,

Sudiarta

et al. 2024

Sustainability

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Improving agricultural productivity to ensure food security while maintaining sustainability is a challenge that needs to be overcome in Bali, Indonesia. Ten farmers in Karangasem Regency, which is among the areas in Bali with a low food security index, were interviewed regarding their agricultural practices and government support for increasing the production of rice and chili—the main crops in the regency—while maintaining sustainability. The interview results revealed that the farmers recognized a lack of sunlight and disease as constraints to cultivation and attempted to improve productivity and control the disease by selecting varieties, cropping systems, and synthetic insecticides based on their previous experiences and the recommendations of agricultural extension workers. The Karangasem Regency Government actively encourages farmers to use biofertilizers and biological control agents to promote sustainable agriculture. Their use to improve rice and chili productivity is important to sustainably increase food security not only in Karangasem Regency but also in Bali Province. Furthermore, since agricultural extension workers are a source of information on agricultural production for farmers, it is important to train them for further extension activities in the future.

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Distribution of Yellow Curly Leaf Disease in Chili Plantations in Southeast Sulawesi and Identification of the Causes

Cited by 3 publications

References 11 publications

Morphometric Analysis of Chili Leaves with Yellow Curly Symptom Using Digital Image Processing Approach and Data Mining Algorithm

Morphometric Analysis of Chili Leaves with Yellow Curly Symptom Using Digital Image Processing Approach and Data Mining Algorithm

Evaluation of three different DNA extraction methods for the detection of Pepper yellow leaf curl virus (PepYLCV) by Polymerase Chain Reaction

Initiatives and Prospects for Sustainable Agricultural Production in Karangasem Regency, Bali, Indonesia

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