Distribution of two transposon-like elements in rice

Wang, Xiaoquan; Shen, Ge-Zhi; Zheng, F. Q.; Wang, Zongyang; Zhang, Jing-Liu; Hong, Meng-Min

doi:10.1016/0168-9452(93)90007-m

Cited by 3 publications

(7 citation statements)

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“…No information is available on the stability of these Wx RNAs. However, given the similarity between the nucleotide sequences of the Wx genes of the group I and group II cultivars (Wang et aL, 1994), the total absence of the 3.3 kb Wx RNA containing intron 1 in the group I cultivars, and the total absence of the 2.3 kb Wx mRNA in the group III cultivars, differential turnover probably is not responsible for the three distinct patterns of Wxtranscripts observed in the 31 cultivars investigated in this study.…”

Section: Discussionmentioning

confidence: 73%

“…Subsequently, a large (1124 bp) intron was identified in the leader region of the rice Wx gene (Figure 1; Wang and Hong, unpublished results). A comparison of the nucleotide sequences of the Wx genes of domesticated rice cultivars (Hanfeng and 232) and wild rice (Dongxiang) revealed the presence of two types of transposon-like elements, designated RTL-1 and RTL-2, located in introns 1, 10, and 13 of the rice Wx gene (Wang et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, an understanding of the genetic control of starch composition in rice seeds will be important in designing breeding and/or (Wang et al, 1990(Wang et al, , 1994. Sequences of the 232 cultivar including the entire intron 1 and the coding region are available from EMBL accession no.…”

Section: Introductionmentioning

confidence: 99%

“…In potato, the expression of a Wx antisense RNA chimeric gene was shown to inhibit GBSS I synthesis, providing direct evidence that GBSS I is the Wx gene product (Visser et al, 1991). We previously cloned and sequenced Wxgenes from three rice cultivars: Oryza sativa subspecies japonica Hanfeng cultivar (Wang et al, 1990), O. sativa subspecies indica 232 cultivar, and O. sativa L. f. spontanea Dongxiang (wild rice) (Wang et al, 1994). The coding region of the Wx gene from the Hanfeng cultivar consists of 13 exons interrupted by 12 introns (Wang et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

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The amylose content in rice endosperm is related to the post‐transcriptional regulation of the waxy gene

Wang¹,

Zheng²,

Shen³

et al. 1995

The Plant Journal

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274

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Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The amylose content in rice endosperm is related to the post‐transcriptional regulation of the waxy gene

Wang¹,

Zheng²,

Shen³

et al. 1995

The Plant Journal

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391

274

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“…The waxy gene of rice encodes starch granule-bound starch synthase (GBSS, or more commonly, Wx protein) which is responsible for the synthesis of amylose in rice endosperm and pollen (Okagaki and Wessler, 1988). We have previously cloned and sequenced the Wx gene from three rice cultivars: Oryza sativa sub-species japonica Hanfeng cultivar (Wang et al, 1990), Oryza sativa sub-species indica 232 cultivar, and Oryza sativa L.f. spontanea Dongxiang which is wild rice (Wang et al, 1994). The coding region of the Wx gene from the Hanfeng cultivar consists of 13 exons interrupted by 12 introns (Wang et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Aberrant splicing of intron 1 leads to the heterogeneous 5′ UTR and decreased expression of waxy gene in rice cultivars of intermediate amylose content

et al. 1998

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SummaryWe have previously reported that three distinct patterns of waxy (Wx) gene transcript accumulation were present in 31 rice cultivars. The cultivars with high amylose content (group I) contain a 2.3 kb mature Wx mRNA, cultivars with intermediate amylose content (group II) produce both a 3.3 kb Wx pre-mRNA, which contains intron 1, and the 2.3 kb Wx mature mRNA, and cultivars with no amylose (group III) accumulate only the 3.3 kb Wx pre-mRNA. Analyses of the cDNAs reveals that four splice donor sites and three splice acceptor sites in intron 1 give rise to six splicing patterns in 2.3 kb Wx mRNA of group II cultivars. In addition, aberrant intron 1 excision causes either deletion of 4 or 5 nucleotides, or addition of 7 and 13 nucleotides at the junction of exon 1 and exon 2 of the 2.3 kb mRNA. In contrast, only one normal splicing pattern (one splice donor site and one splice acceptor site) was found in the 2.3 kb mRNA of group I cultivars. Nucleotide sequences of the Wx intron 1 in group I and group II cultivars differ by 16 individual bases. We suggest that these deletions or additions contribute to inefficient splicing of intron 1 from the 3.3 kb Wx pre-mRNA, as well as an aberrant splicing of the Wx intron 1 to produce the 2.3 kb mRNA with a heterogeneous 5Ј untranslated region (5Ј-UTR). As a consequence, the total amount of translatable Wx mRNA, and therefore the Wx protein and amylose content, are reduced in the group II cultivars compared with the group I cultivars.

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1995

Annu. Rev. Genet.

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The long civilization of China traces its origin to the development of agriculture. Early records and archaeological findings demonstrate the success of plant and animal breeding in contributing to human livelihood and the national economy. Teaching and research in modern genetics began in China in the 1920s with individuals who received advanced training in the West. Over the following 30 years, notable contributions to genetics, both basic and applied, were made by Chinese scientists in universities and research institutions. Unfortunately, these activities were brought to a virtual standstill first by the official advocacy of Lysenkoism, foll owed by ten years of Cultural Revolution. Schools were closed and scientific research was interrupted. Not until 1978 did the universities begin to enroll new students and scientific research, including genetics, again receive endorsement. Since then, Chinese scientists have resumed contacts with col leagues in other countries and publication of their research findings in national and international journals. The material summarized in this article covers a long time span and a broad spectrum of scientific subjects and events. Representative examples of Chinese contributions to various areas of genetics are cited to depict the history, current status, and prospects for the development of genetics in China. After many ups and downs and despite limitations in material resources, the study of genetics in China seems to be on a sound footing, with a relatively complete system in place for training workers in this discipline.

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Distribution of two transposon-like elements in rice

Cited by 3 publications

References 22 publications

The amylose content in rice endosperm is related to the post‐transcriptional regulation of the waxy gene

The amylose content in rice endosperm is related to the post‐transcriptional regulation of the waxy gene

Aberrant splicing of intron 1 leads to the heterogeneous 5′ UTR and decreased expression of waxy gene in rice cultivars of intermediate amylose content

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