Distribution of the professional phagocytic granulocytes of the bony fish gilthead seabream (Sparus aurata L.) during the ontogeny of lymphomyeloid organs and pathogen entry sites

Mulero, Iván; Chaves-Pozo, Elena; Garcı́a-Alcázar, A.; Meseguer, José; Mulero, Víctoriano; Ayala, Alfonsa Garcı́a

doi:10.1016/j.dci.2006.11.011

Cited by 20 publications

(11 citation statements)

References 35 publications

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“…Though renal tubules were present at 7-dpf, cells with hematopoietic morphological features were not observed in the head kidney until 1-wph. This is similar to what has been found in the gilthead seabream, where renal tubules appeared in 2 day old fish, but hematopoietic cells were not present until 4 days later (Mulero et al, 2006). As the juvenile mummichog aged in this study, both the number of renal tubules and the proportion of hematopoietic tissue increased.…”

Section: Discussionsupporting

confidence: 89%

Lymphoid Tissue Ontogeny in the Mummichog, Fundulus heteroclitus

Hunt

Rice

2008

The Anatomical Record

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Most studies that examine the ontogeny of lymphoid organ development in teleostean fishes use species of interest to aquaculture or genetic research and, to date, have focused strictly on marine or freshwater species. The mummichog, Fundulus heteroclitus, also known as the estuarine killifish, is a unique model for studies on developmental immunobiology, because it is euryhaline, has a high degree of thermal tolerance, and has a unique reproductive strategy. Embryonic and larval mummichogs were examined for the ontogeny of lymphoid tissue development. The first lymphoid organ to appear was the head kidney at 1 dph, followed by the spleen at 1 wph, and then the thymus at 3 wph. Rag-1 was partially cloned and sequenced and shown to be highly conserved among other vertebrate Rag-1 genes. Using QT-PCR to monitor the temporal expression of Rag-1, it was shown to reach a maximum intensity at 3 and 4 wph and then to drop to pre-2-wph levels. Overall, this study suggests that juvenile mummichogs do not possess the ability to mount T-or B-cell responses until some time after 5 wph. Even though the estuarine killifish tolerates a wide range of salinities, the developmental patterns of lymphoid tissues are similar to what has been reported for strictly marine (stenohaline) teleosts. Thus, the mummichog should be a convenient model for understanding the developmental immunobiology of most marine teleosts.

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Section: Discussionsupporting

confidence: 89%

Lymphoid Tissue Ontogeny in the Mummichog, Fundulus heteroclitus

Hunt

Rice

2008

The Anatomical Record

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“…However, head kidney is not the only site of hematopoiesis in fish. According to various authors (Glomski et al 1992; Houston et al 1996; Kobayashi et al 2006, 2007, 2008; Zapata et al 2006; Mulero et al 2007 and Santos et al 2011), other organs such as spleen, gut-associated lymphoid tissue (GALT), mucosa-associated lymphoid tissue (MALT), and intertubular tissue of trunk kidney ( mesonephros ) may also show hematopoietic activity. In some fish species, several hematopoietic organs are active, while in the others only one of them (Liu et al 2004; Mulero et al 2007; Patel et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Cadmium and copper reduce hematopoietic potential in common carp (Cyprinus carpio L.) head kidney

Kondera

Witeska

2012

Fish Physiol Biochem

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The effects of cadmium and copper on activity of common carp head kidney hematopoietic tissue were evaluated. The fish were subjected to short-term (3 h, Cd-s and Cu-s) or long-term (4 weeks, Cd-l and Cu-l) exposures to 100 % 96hLC50 or 10 % 96hLC50, respectively. Head kidneys were isolated weekly from 5 fish of each group for 4 weeks (post-short-term exposure and during long-term exposure). Percentage of early blast cells among the hematopoietic precursors was calculated. Proliferative and apoptotic activity were evaluated using immunocytochemical staining for proliferating cell nuclear antigen (PCNA) and caspase 3, respectively. Hematopoietic activity was calculated as the ratio of proliferating to apoptotic cells. All metal exposures induced an increase in frequency of early blast cells. The frequency of proliferating (PCNA-positive) cells also significantly increased. A considerable and significant increase in the frequency of apoptotic cells was the most pronounced effect of metal exposures. Both short-term and long-term treatments caused similar effects, but in case of Cd exposures, the reaction was more pronounced. All metal exposures reduced hematopoietic potential of fish measured as the ratio of proliferating to apoptotic precursor cell frequency. However, in all cases, hematopoietic activity was higher than 1 showing that the rate of repair of hematopoietic tissue prevailed over destruction.

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“…Although there are reports describing organogenesis in different marine species (reviewed by ref. [18]) and on the ontogenesis of important immune related organs and cells in many fish species [16,19,20,21,[23][24][25][26][27][28][29][30][31][32][33][34][35][36][37], very little information on halibut is available.…”

Section: Introductionmentioning

confidence: 99%