Distribution of the beta 1 subgroup of the integrins in human cells and tissues.

Strooper, Bart De; Schueren, B Van der; Jaspers, Martine; Saison, M.; Spaepen, Marijke; Leuven, Fred Van; Berghe, Herman Van den; Cassiman, Jean‐Jacques

doi:10.1177/37.3.2645360

Cited by 68 publications

(23 citation statements)

References 34 publications

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“…These molecules make up the VLA proteins (33). p, is widely distributed in human cells and tissues (34) and may be involved in transmembrane signal transduction via interaction with cytoskeletal elements (35). In our study, p,-integrin (CD29) was present to a greater extent on most cell types in noninflammatory tissues.…”

Section: Discussionmentioning

confidence: 46%

Adhesion molecule expression in human synovial tissue

Johnson

Haines

Harlow

et al. 1993

Arthritis & Rheumatism

206

130

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Objective. We have previously shown that E‐selectin is expressed on endothelium in rheumatoid arthritis (RA) synovial tissues, and hence may be important in recruitment of leukocytes into the inflamed joint. In the present study, we determined whether other cellular adhesion molecules, including selectins and members of the integrin and immunoglobulin supergene families, are expressed in frozen synovium. Methods. We employed immunohistochemical staining to determine the distribution of CD31 (PECAM), CD44 (hyaluronate receptor), CD62 (P‐selectin), Leu‐8 (L‐selectin), and the integrin subunits α5 (VLA‐5), α6 (VLA‐6), β1 (VLA 1–6), and β3 (vitronectin receptor), in synovial tissue from 9 RA and 9 osteoarthritis (OA) patients, and from 3 normal (NL) subjects. Results. P‐selectin was expressed on vascular endothelium in all synovial tissues examined. L‐selectin and α5‐integrin, while expressed on a variety of cell types, were not differentially expressed on RA synovial tissues. Integrin subunits α6 and β1 were down‐regulated on some RA synovial tissue components. In contrast, CD31 was expressed to a greater extent on RA than on OA lining cells and macrophages (P < 0.05). CD44 was expressed to a greater extent on RA or OA macrophages, lining cells, and fibroblasts compared with NL (P < 0.05). Integrin subunit β3 was strongly expressed on RA synovial blood vessels compared with NL (P < 0.05). Conclusion. The expression of integrins VLA 1–6, and selectins P and L is not up‐regulated in RA synovial tissues. CD31 and CD44 are up‐regulated on RA macrophages and lining cells, CD44 on RA fibroblasts, and β3‐integrin on RA blood vessels. The up‐regulation of CD31, CD44, and β3‐integrin in RA synovial tissues may help tip the balance of adhesive interactions toward passage and retention of leukocytes in the inflamed joint.

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Section: Discussionmentioning

confidence: 46%

Adhesion molecule expression in human synovial tissue

Johnson

Haines

Harlow

et al. 1993

Arthritis & Rheumatism

206

130

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“…Most lung cancer cell lines as well as normal bronchial epithlial cells (Strooper et al, 1989) appear to express the A subunit (Feklman et at., 1991). Although several investigators (Ruff & Pert, 1984;Bunn et al, 1985;Ball et at., 1986) have reported the am subunit in a small number of small cell lung cancer lines, very little expression of the P2 subunit was found on small cell lung cancer cells (Feldman et al, 1991).…”

Section: Reagentsmentioning

confidence: 99%

Integrin expression and ability to adhere to extracellular matrix proteins and endothelial cells in human lung cancer lines

Hirasawa

Shijubo²,

Uede³

et al. 1994

Br J Cancer

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“…VLA-4 is a member of the #31 integrins that is expressed by resting lymphocytes, monocytes, as well as by most T and B cell lines (4)(5)(6)(7). Unlike other VLA members, the VLA-4 heterodimer has been implicated in cell-cell adhesion and antibodies against human VLAa4 chain inhibit the attachment oflymphocytes to high endothelial venule cells from Peyer's patches (8,9).…”

Section: Introductionmentioning

confidence: 99%

Upregulated expression and function of VLA-4 fibronectin receptors on human activated T cells in rheumatoid arthritis.

Laffón¹,

García‐Vicuña²,

Humbría³

et al. 1991

J. Clin. Invest.

197

106

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The VLA4 (CD49d/CD29) integrin is a cell surface receptor involved in the interaction of lymphoid cells with both extracellular matrix (ECM) and endothelial cells. We have investigated the expression and function of VLA4 fibronectin (FN) receptors on T cells localized in the inflammed synovium of patients with rheumatoid arthritis (RA). A high proportion of T cells in both synovial membrane (SM) and synovial fluid (SF) expressed the activation antigens AIM (CD69) and gp95/85 (Ea2) as well as an increased number of VLA4a and fil adhesion molecules, as compared with peripheral blood (PB) T cells from the same patients. Furthermore, the majority of these activated SF T cells were able to adhere to a 38-kD FN proteolytic fragment containing the connecting segment-i (CS-i) specifically through VLA4 receptors, whereas a significantly lower proportion of PB T cells displayed this capacity. Therefore, our results show that activated T cells selectively localize at sites of tissue injury in RA disease and provide evidence for the in vivo regulation of the expression and function of the VLA4 integrin. This regulatory mechanism may enable T cells either to facilitate migration or to persist at sites of inflammation. (J. Clin. Invest. 1991. 88:546-552.)

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Distribution of the beta 1 subgroup of the integrins in human cells and tissues.

Cited by 68 publications

References 34 publications

Adhesion molecule expression in human synovial tissue

Adhesion molecule expression in human synovial tissue

Integrin expression and ability to adhere to extracellular matrix proteins and endothelial cells in human lung cancer lines

Upregulated expression and function of VLA-4 fibronectin receptors on human activated T cells in rheumatoid arthritis.

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