Integrin expression and ability to adhere to extracellular matrix proteins and endothelial cells in human lung cancer lines

Hirasawa, Michio; Shijubo, Noriharu; Uede, Toshimitsu; S, Abe

doi:10.1038/bjc.1994.329

Cited by 28 publications

(20 citation statements)

References 47 publications

(26 reference statements)

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“…Most lung cancer cell lines as well as normal bronchial epithelial cells express the β1 integrin subunit [25,27]. The present study confirms that lung cancer cells in culture express a variety of β1 integrins.…”

Section: Discussionsupporting

confidence: 85%

“…However, U-1810 and U-1752 expressed β3 integrins. Several studies have focused on the integrin expression of lung cancer cells but have not clearly shown distinct patterns of integrin expression among various histological types of lung cancer [15,[25][26][27]. Most lung cancer cell lines as well as normal bronchial epithelial cells, however, express the β1 integrin subunit [25,27].…”

Section: Discussionmentioning

confidence: 99%

“…The U-1810 cell line, however, clearly differed from the rest of the lines by lacking expression of β1 integrins. Lung cancer cell lines with a weak or total absence of β1 integrin expression have been described previously [25].…”

Section: Discussionmentioning

confidence: 99%

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Integrin dependent migration of lung cancer cells to extracellular matrix components

Bredin

Kg²,

Hauzenberger³

et al. 1998

Eur Respir J

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Since tumour progression is dependent on the ability of malignant cells to interact with the extracellular matrix (ECM), we have investigated the significance of beta1 and beta3 integrins for migration of lung cancer cells to components of the ECM. In an in vitro hapto- and chemotactic assay system, five cell lines representing the major types of lung cancer were examined: adenocarcinoma (WART); squamous cell carcinoma (U-1752); small cell lung cancer (SCLC) (U-1906, 054 A) and large cell lung cancer (LCLC) (U-1810). Flow cytometric analyses were performed to characterize their integrin expression. U-1906, 054 A, WART and U-1752 all expressed beta1 integrins whereas U-1810 did not. However, U-1810 and U-1752 expressed beta3 integrins. All cell lines except U-1810 and U-1752 showed hapto- and chemotactic motility to fibronectin, laminin and type IV collagen and this motility was beta1 integrin-dependent except in the case of U-1810. However, the hapto- and chemotactic responses differed markedly between the separate cell lines and there was no distinct pattern to separate non-small cell lung cancer (NSCLC) from SCLC. No or very little migration was seen in control experiments with bovine serum albumin (BSA) or serum-free medium alone, indicating that the migration of the lung cancer cells require adhesion molecules, soluble or substratum bound. We have found the involvement of beta1 integrins in lung cancer cell migration in vitro towards fibronectin, laminin and type IV collagen except in the case of U-1810. The U-1810 cell line clearly differed from the rest of the cell lines by lacking expression of beta1 integrins.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

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Integrin dependent migration of lung cancer cells to extracellular matrix components

Bredin

Kg²,

Hauzenberger³

et al. 1998

Eur Respir J

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“…It has been shown that the integrin subunits ␤1 and ␤4 are expressed fairly ubiquitously in lung cancer cells as common subunits, whereas ␤3 and ␤5 subunits are not detected in every lung cancer cell type. 16,17 Our semiquantitative measurement of integrins ␣ v , ␤5 (Figure 3), and ␤3 (data not shown) showed that the expression level of these integrin subunits varied considerably among the cell lines examined. However, the total amount of ␣ v plus ␤5 seemed to correlate with the cell's susceptibility to adenovirus (Figures 1 and 3).…”

Section: Figure 7 Growth Suppression In H460 Cells Infected With Adcamentioning

confidence: 89%

The levels of integrin αvβ5 may predict the susceptibility to adenovirus-mediated gene transfer in human lung cancer cells

Takayama¹,

Ueno

Pei³

et al. 1998

Gene Ther

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Adenovirus-mediated transfer of growth-inhibiting moldependent on the infection efficiency. Growth inhibition ecules, such as p53, shows promise as an effective was induced in all cell lines tested following infection with method of suppressing the growth of cancer cells. As the an adenovirus containing p53, regardless of the genetic basis for in vivo studies, we examined transfection status of their endogenous p53 provided a sufficient efficiency using 15 human lung cancer cell lines that differ amount of p53 protein was expressed. Our results (1) conin their endogenous p53 status. When infected with an firm that the examination of the susceptibility of target canadenovirus expressing bacterial ␤-galactosidase, the difcer cells to an adenovirus is important when considering ferent cell lines showed different levels of ␤-galactosidase performing adenovirus-mediated gene transfer and for evaactivity. We found a correlation between the level of inteluating its therapeutic effects; and (2) suggest that the grin ␣ v ␤5, which is thought to be an adherence receptor quantification of integrin ␣ v ␤5 may be a good way of prefor adenoviruses, and the expression level of the transdicting the susceptibility of cells to adenoviral vectors. ferred gene, suggesting that gene expression is largely

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“…It has also been reported that lung cancer cells adhere to endothelial cells through the b 1 sub-family of integrins [33], indicating their ability to infiltrate endothelial cells, thereby facilitating metastasis. The b 1 sub-family is also known to maintain the motile nature of NSCLC cells by activation of motilitypromoting signals involving Fak and Src kinases [34].…”

Section: Cell -Extracellular Matrix Interactionmentioning

confidence: 99%

Recapitulating tumour microenvironment in chitosan–gelatin three-dimensional scaffolds: an improvedin vitrotumour model

Arya

Sardana

Saxena

et al. 2012

J. R. Soc. Interface.

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Owing to the reduced co-relationship between conventional flat Petri dish culture (twodimensional) and the tumour microenvironment, there has been a shift towards threedimensional culture systems that show an improved analogy to the same. In this work, an extracellular matrix (ECM)-mimicking three-dimensional scaffold based on chitosan and gelatin was fabricated and explored for its potential as a tumour model for lung cancer. It was demonstrated that the chitosan -gelatin (CG) scaffolds supported the formation of tumoroids that were similar to tumours grown in vivo for factors involved in tumour-cell -ECM interaction, invasion and metastasis, and response to anti-cancer drugs. On the other hand, the two-dimensional Petri dish surfaces did not demonstrate gene-expression profiles similar to tumours grown in vivo. Further, the three-dimensional CG scaffolds supported the formation of tumoroids, using other types of cancer cells such as breast, cervix and bone, indicating a possible wider potential for in vitro tumoroid generation. Overall, the results demonstrated that CG scaffolds can be an improved in vitro tool to study cancer progression and drug screening for solid tumours.

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Integrin expression and ability to adhere to extracellular matrix proteins and endothelial cells in human lung cancer lines

Abstract: SaryWe examined the integrin expression

Cited by 28 publications

References 47 publications

Integrin dependent migration of lung cancer cells to extracellular matrix components

Integrin dependent migration of lung cancer cells to extracellular matrix components

The levels of integrin αvβ5 may predict the susceptibility to adenovirus-mediated gene transfer in human lung cancer cells

Recapitulating tumour microenvironment in chitosan–gelatin three-dimensional scaffolds: an improvedin vitrotumour model

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