Distribution of 35 S‐Sulfate within the transseptal ligment of the mouse

J of Periodontal Research

1988

Self Cite

Johnson RB.: Distribution of^H-fucose in the transseptal ligament of the mouse. J Periodont Res 1988; 23: 363-369. The present study demonstrated 'H-fucose distribution in three regions of the transseptal hgament; the middle, mesial (adjaeent to the first molar tooth) and distal (adjacent to the second molar tooth) thirds; of 6-week-and 6-month-old Swiss mice. Incorporation of the isotope was rapid and maximal peaks occurred at 3 hours in most regions of 6-month and at 12 h in most regions of 6-wk animals: highest grain counts were over the middle third of the ligament of 6-wk animals (p< 0.001). During incorporation, mean grain counts were significantly different as a function of age (p<0.001), region (p<0.01) and time (p<0.001) and there was a significant interaction between region and time (p< 0.005). Grain removal occurred at a slower rate: there were significant differences in mean grain counts as a function of age (p<0.001), region (p<0.001) and time (p<0.00!) and a significant interaction between time and region (p<0.001) and between time, region and age (p<0.001). The highest rate of grain removal was in the middle third of the ligament of 6-wk animals. Half-hfe of labeled glycoproteins was shortest in the ligament of 6-wk animals. The study suggested regional differences in metabolism of fucose-containing glycoproteins of the transseptai ligament: highest turnover rates in the 1) middle third, and 2) young animals.

Section: Discussioncontrasting

confidence: 62%

Distribution of ³H‐fucose in the transseptal ligament of the mouse

J of Periodontal Research

1988

Self Cite

“…It is well known that both the PDL and the alveolar wall have regional variations in collagenous protein metabolism (Diaz, 1978;Beertsen, 1979;Vignery and Baron, 1980;Johnson, 1986;Nemeth et al, 1989). However, previous studies have not quantified the regional variations in sGAG concentrations within either the PDL or the adjacent alveolar wall (Baumhammers and Stallard, 1968;Bartold, 1987;Kirkham et al, 1992;Okazaki et al, 1992;Kagayama et al, 1996;Evefls et al, 1998), even though regional variations in their concentrations have been reported within the transseptal ligament (Johnson, 1989). Our study extends previous studies by reporting regional variability in the comparative sGAG and collagenous protein metabolism within both the PDL and the adjacent alveolar bone.…”

Section: Discussionmentioning

confidence: 99%

“…There are qualitative (Baumhammers and Stallard,1968; Lau et al,1983) but no quantitative studies of regional variations in the metabolism of 35 S‐sulfate (which is a marker for sGAG metabolism) within the PDL during physiologic tooth movements, although these data are available for the PDL collagenous proteins (Rippin,1976; Diaz,1978; Johnson,1986, 2005). A previous study reported significant regional variations in 35 S‐sulfate metabolism within the transseptal ligament of the mouse during physiologic drift of the molar teeth (Johnson,1989), and we hypothesized that regional variations in the distribution and metabolism of sGAG would also be evident within the PDL of the rat molar dentition. The objective of the present study was to compare the collagenous protein and sGAG metabolism at three levels of the PDL (cervical, middle, and apical), adjacent to either the mesial or distal surfaces of the interdental septum, during physiological drift of the rat molar teeth.…”

mentioning

confidence: 99%

Comparative35S-sulfate and3H-proline metabolism within the interdental septal bone and adjacent periodontal ligament

2006

Anat. Rec.

Self Cite

Tooth movements require rapid remodeling of the periodontal ligament (PDL) and adjacent alveolar bone. Our objective was to compare the regional metabolism of sulfated-glycosaminoglycans (sGAG) within the PDL and adjacent alveolar bone and compare it to the metabolism of collagenous proteins using radioautographic techniques. Rats were injected with either 3 H-proline or 35 S-sulfate and maxillae were removed at 1, 6, and 12 hr 1-7 days later. Silver grains were counted over the PDL and adjacent alveolar bone and the incorporation and removal rates for each radioisotope were determined. In general, net collagenous protein incorporation and removal were greatest within the distal and net sGAG incorporation and removal were greatest within the mesial compartments of the periodontium. The rate of removal of 3 H-proline was significantly greater within the distal alveolar bone surface than the adjacent PDL at all levels (P Ͻ 0.001). In contrast, the rate of removal of 35 S-sulfate was significantly greater in the PDL than within the adjacent mesial surface of the interdental septum at all levels (P Ͻ 0.001). The mesial surfaces of the interdental septum had a slower rate of removal of both isotopes than distal surfaces at all levels (P Ͻ 0.001). Our data suggest significant regional differences in the metabolism of 35 S-sulfate and 3 H-proline within the PDL and alveolar bone, which likely result from the characteristics of the forces produced by the adjacent teeth and may be a factor in the remodeling of the alveolar wall coincident to tooth movement. Key words: alveolar bone; periodontal ligament; glycosaminoglycans; physiological tooth movement; collagenous proteinsThe periodontal ligament (PDL) anchors the root of the tooth to the adjacent alveolar bone and also separates the tooth root from the alveolar wall. The structural components of the PDL include a fibrous component, providing structural support for the teeth, and noncollagenous matrix interspersed between the collagen fibers, which is composed of proteoglycans and glycoproteins (Embery et al., 1995). The fibrous component resists tension forces from adjacent tooth roots and the noncollagenous matrix portion resists the compression forces by forming a biphasic medium with water, giving viscoelastic properties to the PDL (Mow et al., 1984). While there are significant regional differences in the metabolism of collagenous proteins within the PDL, there is little information about the regional metabolism of PDL proteoglycans and glycosaminoglycans. Accurate knowledge of this regional variability

Distribution of ³H‐proline within transseptal fibers of the rat following release of orthodontic forces

Row

1990

Am. J. Anat.

Maxillary right first molar teeth of rats were tipped mesially with an orthodontic appliance for 2 weeks (experimental group), 3H-proline was injected, and orthodontic forces were removed 6 hr later (time 0). The contralateral molar teeth of treated (internal control group) and age- and weight-matched untreated animals (external control group) were also studied. Diastemata were created between the molar teeth by the orthodontic appliance, and transseptal fibers between first and second (P less than 0.001) and second and third molars (P less than 0.005) were significantly lengthened as compared to external and internal controls at time 0. Diastemata between molar teeth were closed 5 days after removal of orthodontic force. Transseptal fibers adjacent to the source of the orthodontic force (mesial region) had the highest mean number of 3H-proline-labeled proteins at time 0 and at all times following removal of the force (P less than 0.001), and had the highest rate of labeled protein removal (P less than 0.001). Half-lives for removal of 3H-proline-labeled transseptal fiber proteins were significantly greater in mesial and distal regions and significantly less in middle regions of experimentals than in corresponding regions of external controls (P less than 0.001). These data suggest the following: 1) transseptal fibers adjust their length by rapid remodeling in regions experiencing a tensile force; 2) collagenous protein turnover within the middle third of the transseptal fibers is more rapid subsequent to release of orthodontic force than during normal physiologic drift, suggesting that this region adapts rapidly to changes in adjacent tooth position and that these fibers do not play a significant role in relapse of orthodontically relocated teeth; and 3) significant differences in turnover rates of 3H-proline-labeled transseptal ligament proteins of external and internal control quadrants suggest that tooth movement produces both local and systemic effects on collagenous protein metabolism.