Distribution of sero-biotypes of Campylobacter jejuni and C. coli isolated from paediatric patients

Lastovica, Albert J.; Roux, E. Le; Congi, R V; Penner, J L

doi:10.1099/00222615-21-1-1

Cited by 33 publications

(14 citation statements)

References 13 publications

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“…Isolates from a larger number of patients would be necessary to study this possible relationship in more detail. Among children of the age 0–10 years, Lastovica et al [54]also found differences between HS‐serotypes and age group of the children.…”

Section: Discussionmentioning

confidence: 97%

Distribution of serotypes ofCampylobacter jejuniandC. colifrom Danish patients, poultry, cattle and swine

Nielsen¹,

Engberg

Madsen

1997

FEMS Immunol Med Microbiol

128

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The number of human cases of enteritis caused by Campylobacter jejuni and C. coli is increasing in Denmark and other European countries. No systemic typing has earlier been performed on Campylobacter isolates of Danish origin. The primary purpose of this study was to provide a serotype distribution of Campylobacter isolates from Danish patients and the major food production animals. In addition, the occurrence of intestinal carriers of thermophilic campylobacters among these food production animals was examined. In a nationwide survey, the individual isolation rate was 36% for broiler chickens, 47% for cattle and 46% for swine when sampled at the slaughterhouse. C. jejuni accounted for 83-91% of the thermophilic Campylobacter spp. in broiler chickens and cattle, whereas 95% of the isolates from swine was C. coli. In human patients with Campylobacter enteritis, 94% of the isolates were C. jejuni and 6% were C. coli. Heat-stable serotyping (the 'Penner scheme') was performed on a total of 398 isolates from the four sources: human patients (n = 145), broiler chickens (n = 94), swine (n = 111) and cattle (n = 48). Among human isolates, serotype O:1,44, O:2 and the O:4-complex accounted for 62% of the C. jejuni isolates. These serotypes were also common in samples from broilers and cattle. In swine, C. coli O:30 and O:46 were most common. The serotype distribution of human clinical isolates showed large overlap with the serotype distribution of campylobacters in cattle and chickens, and on this basis both could be major sources of human campylobacteriosis.

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Section: Discussionmentioning

confidence: 97%

Distribution of serotypes ofCampylobacter jejuniandC. colifrom Danish patients, poultry, cattle and swine

Nielsen¹,

Engberg

Madsen

1997

FEMS Immunol Med Microbiol

128

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“…The data represent a total of >16,000 strains from developed countries (Penner and Hennessy, 1980; McMyne et al, 1982; Penner et al, 1983; Jones et al, 1985; Patton et al, 1985; Mills et al, 1991; Albert et al, 1992; Skirrow et al, 1993; Marshall et al, 1994; Owen et al, 1994; Nielsen et al, 1997, 2006; Frost et al, 1998; McKay et al, 2001; Oza et al, 2002; Wareing et al, 2002; Woodward and Rodgers, 2002; Karenlampi et al, 2003; Siemer et al, 2004; Cornelius et al, 2005; Fussing et al, 2007; McTavish et al, 2008); and far fewer strains (711) from less developed countries, including Central Africa (Georges-Courbot et al, 1989), South Africa (Lastovica et al, 1986), South Africa (Lastovica et al, 1986), Egypt (Poly et al, 2011), Bangladesh (Neogi and Shahid, 1987), Kuwait (Sjogren et al, 1989), and Thailand (Poly et al, 2011). …”

Section: Cps Typing By Multiplex Pcrmentioning

confidence: 99%

Campylobacter Polysaccharide Capsules: Virulence and Vaccines

Guerry¹,

Poly²,

Riddle³

et al. 2012

Front. Cell. Inf. Microbio.

124

129

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Campylobacter jejuni remains a major cause of bacterial diarrhea worldwide and is associated with numerous sequelae, including Guillain Barré Syndrome, inflammatory bowel disease, reactive arthritis, and irritable bowel syndrome. C. jejuni is unusual for an intestinal pathogen in its ability to coat its surface with a polysaccharide capsule (CPS). These capsular polysaccharides vary in sugar composition and linkage, especially those involving heptoses of unusual configuration and O-methyl phosphoramidate linkages. This structural diversity is consistent with CPS being the major serodeterminant of the Penner scheme, of which there are 47 C. jejuni serotypes. Both CPS expression and expression of modifications are subject to phase variation by slip strand mismatch repair. Although capsules are virulence factors for other pathogens, the role of CPS in C. jejuni disease has not been well defined beyond descriptive studies demonstrating a role in serum resistance and for diarrhea in a ferret model of disease. However, perhaps the most compelling evidence for a role in pathogenesis are data that CPS conjugate vaccines protect against diarrheal disease in non-human primates. A CPS conjugate vaccine approach against this pathogen is intriguing, but several questions need to be addressed, including the valency of CPS types required for an effective vaccine. There have been numerous studies of prevalence of CPS serotypes in the developed world, but few studies from developing countries where the disease incidence is higher. The complexity and cost of Penner serotyping has limited its usefulness, and a recently developed multiplex PCR method for determination of capsule type offers the potential of a more rapid and affordable method. Comparative studies have shown a strong correlation of the two methods and studies are beginning to ascertain CPS-type distribution worldwide, as well as examination of correlation of severity of illness with specific CPS types.

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“…In this study, we investigated the rRNA gene restriction patterns (ribopatterns), genomic RAPD-profiles and plasmid profiles of strains of C. jejuni within Penner serotypes 01 and 02, which are two of the most prevalent serotypes in the UK [1] and South Africa [28]. The aim was to determine which method or combination of methods was most discriminatory and reproducible for general typing purposes in epidemiological studies.…”

Section: Introductionmentioning

confidence: 99%

Molecular subtyping by genome and plasmid analysis ofCampylobacter jejuniserogroups O1 and O2 (Penner) from sporadic and outbreak cases of human diarrhoea

Fayos

Owen²,

Hernández³

et al. 1993

Epidemiol. Infect.

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SUMMARYRibosomal RNA gene patterns, randomly amplified polymorphic genomic DNA (RAPD) profiles and plasmid profiles were used to discriminate between 28 strains of Campylobacterjejuni serogroups 01 and 02 (Penner). Most isolates were biotype I (Lior). The strains were representative isolates from a UK school outbreak of enteritis (7 cases) and from 21 sporadic human cases of enteritis in 4 countries. The molecular techniques discriminated to various degrees between strains in each of the serogroups. The outbreak strains were homogeneous in most molecular features but a variety of types was detected amongst the isolates from the sporadic cases. Five groups of two or more strains with identical ribopatterns were identified and within each, strains from different patients were homogenous with respect to serogroup. RAPD profile typing based on numerical analysis generally matched ribotyping. Plasmid profiling overall gave least discrimination but was useful in separating some strains similar in other features. We concluded that optimal discrimination of C. jejuni could best be achieved using a combination of phenotypic and genotypic properties. Hae III ribotyping was the single most discriminatory and reproducible technique investigated. Several strains of C. jejuni from sporadic infections had similar molecular profiles which have potential for general typing purposes.

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Distribution of sero-biotypes of Campylobacter jejuni and C. coli isolated from paediatric patients

Cited by 33 publications

References 13 publications

Distribution of serotypes ofCampylobacter jejuniandC. colifrom Danish patients, poultry, cattle and swine

Distribution of serotypes ofCampylobacter jejuniandC. colifrom Danish patients, poultry, cattle and swine

Campylobacter Polysaccharide Capsules: Virulence and Vaccines

Molecular subtyping by genome and plasmid analysis ofCampylobacter jejuniserogroups O1 and O2 (Penner) from sporadic and outbreak cases of human diarrhoea

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