Distribution of serotypes of<i>Campylobacter jejuni</i>and<i>C. coli</i>from Danish patients, poultry, cattle and swine

Nielsen, Eva Møller; Engberg, Jørgen; Madsen, Mogens

doi:10.1111/j.1574-695x.1997.tb01071.x

Cited by 128 publications

(11 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found high subtype diversity among C. jejuni isolates from cattle at each CFO, and there was no significant change in subtype diversity between or within CFOs at the arrival and interim sample times. Others have shown that C. jejuni strains present in cattle are highly genetically diverse and that a relatively small number of highly prevalent subtypes predominate (8,35,36); the findings of the current study confirm this observation across a much larger data set. We identified five CGF subtype clusters that were most prevalent among C. jejuni strains isolated from beef cattle.…”

Section: Mutation Bsupporting

confidence: 88%

Subtype-Specific Selection for Resistance to Fluoroquinolones but Not to Tetracyclines Is Evident in Campylobacter jejuni Isolates from Beef Cattle in Confined Feeding Operations in Southern Alberta, Canada

Webb

Selinger

Taboada

et al. 2018

Appl Environ Microbiol

View full text Add to dashboard Cite

was longitudinally isolated from beef cattle housed in four confined feeding operations (CFOs) in Southern Alberta, Canada, over 18 months. All of the cattle were administered a variety of antimicrobial agents (AMAs) nontherapeutically and metaphylactically during their time in the CFOs. In total, 7,966 isolates were recovered from cattle. More animals were colonized by the bacterium after>60 days in the CFO (interim) than were individuals upon entry at the CFO (arrival). Subtyping and resistance to seven AMAs were determined for 1,832 (23.0%) and 1,648 (20.7%) isolates, respectively. Increases in the proportion of isolates resistant to tetracycline were observed at all four CFOs between sample times and to ciprofloxacin and nalidixic acid at one or more CFOs. The vast majority of isolates resistant to tetracycline carried , whereas ciprofloxacin resistance was predominantly attributed to mutations in the gene. Although considerable diversity was observed, a majority of isolates belonged to one of five predominant subtype clusters. There was no difference in subtype diversity by CFO, but the population structure differed between sample times. Selection for resistance to ciprofloxacin and nalidixic acid was subtype dependent, whereas selection for resistance to tetracycline was not. The findings indicate that a proportion of cattle entering CFOs carry resistant subtypes, and the characteristics of beef cattle CFOs facilitate transmission/proliferation of diverse subtypes, including those resistant to AMAs, which coupled with the densities of CFOs likely contribute to the high rates of cattle-associated campylobacteriosis in Southern Alberta. A small proportion of cattle entering a CFO carry , including subtypes resistant to AMAs. The large numbers of cattle arriving from diverse locations at the CFOs and intermingling within the CFOs over time, coupled with the high-density housing of animals, the high rates of transmission of subtypes among animals, and the extensive use of AMAs merge to create an ideal situation where the proliferation of diverse antimicrobial-resistant subtypes is facilitated. Considering that Southern Alberta reports high rates of campylobacteriosis in the human population and that many of these clinical cases are due to subtypes associated with cattle, it is likely that the characteristics of beef cattle CFOs favor the propagation of clinically relevant subtypes, including those resistant to medically important AMAs, which constitute a risk to human health.

show abstract

Section: Mutation Bsupporting

confidence: 88%

Subtype-Specific Selection for Resistance to Fluoroquinolones but Not to Tetracyclines Is Evident in Campylobacter jejuni Isolates from Beef Cattle in Confined Feeding Operations in Southern Alberta, Canada

Webb

Selinger

Taboada

et al. 2018

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…However, although Jensen et al (2006) showed that the paddock environment of organic pigs was contaminated by non-pig strains, e.g., by wild fauna strains, they did not demonstrate that these strains could contaminate pigs, at least not C. jejuni strains. Pigs show a dominance of C. coli (Nielsen et al, 1997; Alter et al, 2005; Boes et al, 2005) and C. jejuni may co-exist with C. coli in pigs, but C. jejuni is typically present at in numbers that are 10–100-fold lower than C. coli (Madden et al, 2000; Jensen et al, 2005). In our study, we tested only two to three isolates per farm which may explain also why we never detected C. jejuni .…”

Section: Discussionmentioning

confidence: 99%

No Clear Differences between Organic or Conventional Pig Farms in the Genetic Diversity or Virulence of Campylobacter coli Isolates

et al. 2017

View full text Add to dashboard Cite

To evaluate the impact of pig farm management on the genetic diversity and on the virulence of Campylobacter coli, we characterized isolates from 19 organic pig farms (62 isolates) and from 24 conventional pig farms (58 isolates). The 120 C. coli isolates were typed using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) and the presence of nine virulence genes was screened using real-time PCR. The capacity of adhesion and invasion of 61 isolates (32 from organic and 29 from conventional farms) were then tested on human intestinal Caco-2 cells. A total of 59 PFGE types and of 50 sequence types (STs) were identified. Twelve PFGE types and nine STs, accounting for 34 and 41.6% of the isolates, respectively, were common between the two production systems with ST854 dominating (18.3% of the isolates). Twenty-nine PFGE types and 25 STs were only found in isolates from organic farms, and 18 PFGE types and 16 STs from conventional farms. No significant differences were found in diversity despite the differences in rearing systems, except at the locus level for the glnA, gltA, and uncA genes. All isolates, regardless of their origin, carried the ceuE, iam, ciaB, and flaA genes and more than 95% of the isolates carried the cadF and cdtABC genes. No significant differences were found in pathogenicity between the two farming systems. The pathogenicity of the C. coli isolates was low compared to C. jejuni control strains tested. The plasmid gene virb11 was detected in only 13 isolates from organic farms; these isolates showed greater invasion capacity than those without this gene. Our study indicates that pig farm management does not significantly affect the diversity and the virulence of Campylobacter coli isolated from pigs. The common genotypes between conventional and organic farms may indicate that some genotypes are adapted to pigs.

show abstract

“…There is some host association in Campylobacter species. In a Danish survey from samples collected at abattoirs, 83%-91% of Campylobacter isolates from poultry and cattle were identified as C. jejuni, whereas 95% of isolates from swine were identified as C. coli (Nielsen, Engberg, & Madsen, 1997). In a Swedish study, C. lari was most common in migrating birds (Waldenstr€ om et al, 2002), whereas dogs are mostly colonized with C. upsaliensis (Olsson et al, 2003;Sandberg, Bergsjø, Hofshagen, Skjerve, & Kruse, 2002;Wieland et al, 2005).…”

Section: Description Of the Agent Taxonomy And Classificationmentioning

confidence: 99%

Knowledge gaps in control ofCampylobacterfor prevention of campylobacteriosis

Hansson

Sandberg

Habib

et al. 2018

Transbound Emerg Dis

139

112

View full text Add to dashboard Cite

Campylobacteriosis is an important, worldwide public health problem with numerous socio-economic impacts. Since 2015, approximately 230,000 cases have been reported annually in Europe. In the United States, Australia and New Zealand, campylobacteriosis is the most commonly reported disease. Poultry and poultry products are considered important sources of human infections. Poultry meat can become contaminated with Campylobacter during slaughter if live chickens are intestinal carriers. Campylobacter spp. can be transferred from animals to humans through consumption and handling of contaminated food products, with fresh chicken meat being the most commonly implicated food type. Regarding food-borne disease, the most important Campylobacter species are Campylobacter jejuni and Campylobacter coli. In humans, clinical signs of campylobacteriosis include diarrhoea, abdominal pain, fever, headache, nausea and vomiting. Most cases of campylobacteriosis are sporadic and self-limiting, but there are post-infection complications, for example, Guillain-Barrés syndrome. This review summarizes an analysis undertaken by the DISCONTOOLS group of experts on campylobacteriosis. Gaps were identified in: (i) knowledge of true number of infected humans; (ii) mechanisms of pathogenicity to induce infection in humans; (iii) training to prevent transfer of Campylobacter from raw to ready-to-eat food; (iv) development of effective vaccines; (v) understanding transmission routes to broiler flocks; (vi) knowledge of bacteriocins, bacteriophages and antimicrobial peptides as preventive therapies; (vii) ration formulation as an effective preventive measure at a farm level; (viii) development of kits for rapid detection and quantification of Campylobacter in animals and food products; and (ix) development of more effective antimicrobials for treatment of humans infected with Campylobacter. Some of these gaps are relevant worldwide, whereas others are more related to problems encountered with Campylobacter in industrialized countries.

show abstract

Distribution of serotypes ofCampylobacter jejuniandC. colifrom Danish patients, poultry, cattle and swine

Cited by 128 publications

References 49 publications

Subtype-Specific Selection for Resistance to Fluoroquinolones but Not to Tetracyclines Is Evident in Campylobacter jejuni Isolates from Beef Cattle in Confined Feeding Operations in Southern Alberta, Canada

Subtype-Specific Selection for Resistance to Fluoroquinolones but Not to Tetracyclines Is Evident in Campylobacter jejuni Isolates from Beef Cattle in Confined Feeding Operations in Southern Alberta, Canada

No Clear Differences between Organic or Conventional Pig Farms in the Genetic Diversity or Virulence of Campylobacter coli Isolates

Knowledge gaps in control ofCampylobacterfor prevention of campylobacteriosis

Contact Info

Product

Resources

About