Distribution of RTX toxin genes in strains of [Actinobacillus] rossii and [Pasteurella] mairii

Mayor, Désirée; Korczak, Bożena; Bisgaard, Magne; Frey, Joachim; Kuhnert, Peter

doi:10.1016/j.vetmic.2006.03.003

Cited by 12 publications

(13 citation statements)

References 17 publications

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“…The swine nasal cavities and tonsils are heavily colonized with several members of the commensal flora, such as Actinobacillus minor, Actinobacillus porcinus, and Actinobacillus indolicus (46), as well as with other nonpathogenic Actinobacillus isolates antigenically and biochemically similar to A. pleuropneumoniae and with an atypical toxin profile (1). For instance, Actinobacillus rossii produces variants of the ApxIII toxin, either alone or in combination with ApxII (7,47), while A. suis produces RTX toxins that are identical to ApxII and ApxI (40,48). In addition, the Apx toxins are related to RTX toxins from other species, such as Hly of E. coli and the leukotoxins of Pasteurella haemolytica and Actinobacillus actinomycetemcomitans (9).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Detection of Antibodies against Apx Toxins ApxI, ApxII, ApxIII, and ApxIV in Pigs with Known and Unknown Actinobacillus pleuropneumoniae Exposure Using a Multiplexing Liquid Array Platform

Giménez-Lirola¹,

Jiang²,

Sun³

et al. 2013

Clin. Vaccine Immunol.

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c Surveillance for the presence of Actinobacillus pleuropneumoniae infection in a population plays a central role in controlling the disease. In this study, a 4-plex fluorescent microbead-based immunoassay (FMIA), developed for the simultaneous detection of IgG antibodies to repeat-in-toxin (RTX) toxins (ApxI, ApxII, ApxIII, and ApxIV) of A. pleuropneumoniae, was evaluated using (i) blood serum samples from pigs experimentally infected with each of the 15 known A. pleuropneumoniae serovars or with Actinobacillus suis, (ii) blood serum samples from pigs vaccinated with a bacterin containing A. pleuropneumoniae serovar 1, 3, 5, or 7, and (iii) blood serum samples from pigs with an unknown A. pleuropneumoniae exposure status. The results were compared to those obtained in a previous study where a dual-plate complement fixation test (CFT) and three commercially available enzyme-linked immunosorbent assays (ELISAs) were conducted on the same sample set. On samples from experimentally infected pigs, the 4-plex Apx FMIA detected specific seroconversion to Apx toxins as early as 7 days postinfection in a total of 29 pigs inoculated with 14 of the 15 A. pleuropneumoniae serovars. Seroconversion to ApxII and ApxIII was detected by FMIA in pigs inoculated with A. suis. The vaccinated pigs showed poor humoral responses against ApxI, ApxII, ApxIII, and ApxIV. In the field samples, the humoral response to ApxIV and the A. pleuropneumoniae seroprevalence increased with age. This novel FMIA (with a sensitivity of 82.7% and a specificity of 100% for the anti-ApxIV antibody) was found to be more sensitive and accurate than current tests (sensitivities, 9.5 to 56%; specificity, 100%) and is potentially an improved tool for the surveillance of disease and for monitoring vaccination compliance.

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Section: Discussionmentioning

confidence: 99%

Simultaneous Detection of Antibodies against Apx Toxins ApxI, ApxII, ApxIII, and ApxIV in Pigs with Known and Unknown Actinobacillus pleuropneumoniae Exposure Using a Multiplexing Liquid Array Platform

Giménez-Lirola¹,

Jiang²,

Sun³

et al. 2013

Clin. Vaccine Immunol.

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“…rossii from the vagina of a sow in 1972 and subsequently it was isolated from the stomach of aborted pig fetuses in 1985 . Phenotypically, the bacteria cannot be differentiated from [ P. ] aerogenes , hence the need for 16S RNA gene analysis . Strictly speaking, [ A. ]…”

Section: Discussionmentioning

confidence: 99%

“…Strictly speaking, [ A. ] rossii has been excluded from the Actinobacillus sensu stricto on the basis of genotypic studies, so the genus name is presented in square brackets . The type strain of [ A. ]…”

Section: Discussionmentioning

confidence: 99%

“…The type strain of [ A. ] rossii has been shown to contain apxII and apxIII operons, known to be involved in the pathogenicity of A. pleuropneumoniae, but the link between the presence of apx genes and abortion in pigs has not been elucidated …”

Section: Discussionmentioning

confidence: 99%

“…rossii in abortion is unknown. Reports in the literature about these organisms are very rare and it is suspected that their clinical importance is under‐rated . There is one report of [ A. ]…”

Section: Discussionmentioning

confidence: 99%

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