Distribution of Quinolinate Phosphoribosyl-Transferase in Animals, Plants and Microorganisms

Inhibition of Hog Liver Crystalline Quinolinate Phosphoribosyltransferase by Nucleotides, Quinolinate Analogues and Sulfhydryl Reagents

“…Details of the assay procedure were described in a previous paper. 3) Effect of various compounds on the enzyme activity. Various compounds were separately added to the standard assay reaction mixture, and the activity was assayed under the standard assay conditions.…”

Purification and Some Properties of Nicotinate Phosphoribosyltransferase from Hog Liver

“…This reaction is catalyzed by QPRTase (Ee 2.4.2.19), which is widely distributed in microorganisms, plants, and animals. 7 ) NaMN is also the common intermediate leading to NAD in one of the salvage pathways, and NaMN is the first niacin-active compound in the de novo biosynthetic pathways. QPRTase, which catalyzes the formation of NaMN from quinolinic acid and PRPP, was reported as a functional key enzyme in the de novo NAD biosynthetic pathway8) and has been precisely studied and reported by Iwai et al 9 -11) Besides the de novo biosynthesis ofNAD, NAD is also biosynthesized via two salvage pathways.…”

Influence of Dietary Change from a Niacin- and Protein-free Diet to a Niacin-free 30% Casein Diet on Niacin Formation from Tryptophan in Rats

“…Then the volume was adjusted to ten times the volume of the weight of the organ used. 23 ) Kynurenine aminotransferase was measured by the increase in absorbance at 330nm originating from kynurenic acid formation.2 4 ) Kynurenine hydrolase activity was assayed by measuring anthranilate fluorometrically.25) ACMSDase activity was measured by the method of Ichiyama et a1 26 ) QPRTase activity was assayed according to the method described by Iwai and Taguchi 27 ) …”