Distribution of Pathogenicity Island Markers and H-Antigen Types of <i>Escherichia coli</i> O25b/ST131 Isolates from Patients with Urinary Tract Infection in Iran

Rasoulinasab, Masoumeh; Shahcheraghi, Fereshteh; Feizabadi, Mohammad Mehdi; Nikmanesh, Bahram; Hajihasani, Azade; Sabeti, Shahram; Aslani, Mohammad Mehdi

doi:10.1089/mdr.2019.0485

Cited by 5 publications

(7 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with our results, previous Fig. 2 Comparison of the frequency of antibiotic resistance and virulence genes in O25-B2-ST131 and non-O25-B2-ST131 strains studies reported a high frequency of antibiotic resistance in O25-B2-ST131 strains which turned them into a clinical challenge [40,44,50]. The MIC results showed that none of the isolates, even E. coli O25-B2-ST131 strains, were resistant to colistin, and colistin was the most effective antibiotic against E. coli isolates from UTI.…”

Section: Discussionsupporting

confidence: 91%

“…It was also found that the frequency of the majority of virulence factors in O25-B2-ST131 was higher compared to non-O25-B2-ST131 strains, which indicates the high pathogenicity of O25-B2-ST131. As with our results, previous studies have agreed that the virulence factors of O25-B2-ST131 strains are significant as a dangerous clone type and that these strains are considered as a therapeutic challenge [40,50]. Although the frequency of mcr genes in E. coli ST131 strains is very low, studies have reported the prevalence of this gene in E. coli ST131 strains isolated from animal and environmental samples [60,61].…”

Section: Discussionsupporting

confidence: 91%

“…In the current study, based on the presence of pabB gene using allele-specific PCR, 31 (25.83%) strains were subsequently assigned as O25-B2-ST131 E. coli. However, in line with the results of our study, Rasoulinasab et al reported a prevalence of 26.9% of E. coli O25b/ ST131 in patients with urinary tract infection in Iran [40]. However, the ST131 outbreak has occasionally been reported around the world, including Japan (10%), Denmark (38%) [41], and Australia (51%) [42].…”

Section: Discussionsupporting

confidence: 91%

“…Antibiotic resistance studies have been performed in O25-B2-ST131 strains which confirm our results. These studies have shown that resistance and the presence of genes involved in resistance are higher in O25-B2-ST131 strains [40,50,62]. It is also shown that E. coli ST131 has a wide range of virulence and resistance genes located on plasmids with high transmissibility that has a global spread [39].…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

The genotypic and phenotypic characteristics contributing to high virulence and antibiotics resistance in Escherichia coli O25-B2-ST131 in comparison to non- O25-B2-ST131

Shahbazi

Salmanzadeh-Ahrabi²,

Aslani³

et al. 2023

BMC Pediatr

Self Cite

View full text Add to dashboard Cite

Background Escherichia coli serogroup O25b-sequence type 131 (E. coli O25-B2-ST131) is considered as multidrug-resistant and hypervirulent organism. There is lack of data about involvement of this pathogen in the children’s infection. In this study, the prevalence, and clonality, virulence capacity, and antibiotic resistance phenotype and genotype of E. coli O25-B2-ST131 compared with non-O25-B2-ST131 isolates were investigated in children with urinary tract infection in Tehran, Iran. Methods The E. coli isolates from urine samples were identified using conventional microbiological methods. Characterization of E. coli O25-B2-ST131 clone, antibiotic susceptibility, biofilm formation, ESBLs phenotype and genotype, serum resistance, hemolysis, hydrophobicity, and formation of curli fimbriae were done using conventional microbiological and molecular methods. Clonality of the isolates was done by rep-PCR typing. Results Among 120 E. coli isolates, the highest and lowest antibiotic resistance was detected against ampicillin (92, 76.6%) and imipenem 5, (4.1%), respectively. Sixty-eight (56.6%) isolates were ESBL-producing and 58 (48.3%) isolates were considered as multi-drug resistance (MDR). The prevalence of ESBL-producing and MDR isolates in O25-B2-ST131 strains was higher compared with the non-O25-B2-ST131 strains (p value < 0.05). O25-B2-ST131 strains showed significant correlation with serum resistance and biofilm formation. Amongst the resistance and virulence genes, the prevalence of iucD, kpsMTII, cnf1, vat, blaCTX-M-15, and blaSHV were significantly higher among O25-B2-ST131 isolates in comparison with non-O25-B2-ST131 isolates (p value < 0.05). Considering a ≥ 80% homology cut-off, fifteen different clusters of the isolates were shown with the same rep-PCR pattern. Conclusions Our results confirmed the involvement of MDR-ESBLs producing E. coli strain O25-B2-ST131 in the occurrence of UTIs among children. Source tracking and control measures seem to be necessary for containment of the spread of hypervirulent and resistance variants in children.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The genotypic and phenotypic characteristics contributing to high virulence and antibiotics resistance in Escherichia coli O25-B2-ST131 in comparison to non- O25-B2-ST131

Shahbazi

Salmanzadeh-Ahrabi²,

Aslani³

et al. 2023

BMC Pediatr

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the current study, antimicrobial resistance was more frequent in the O25b/ST131 clones than non-O25b/ST131 isolates and high resistance to all four generations of cephalosporins was detected. Furthermore, two studies in the United Kingdom and Iran reported the same cephalosporins with resistance rates of 68% and 49.5%, respectively [ 37 , 38 ]. Moreover, there are several reports in recent years pointed out the importance of ST131 as a major clone for extraintestinal E. coli infections [ 8 , 39 – 41 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates

Khoshbayan

Zadeh

Moghadam

et al. 2022

Ann Clin Microbiol Antimicrob

View full text Add to dashboard Cite

Background Escherichia coli (E. coli) O25b/ST131 clone causes urinary tract infection (UTI) and is associated with a broad spectrum of other infections, such as intra-abdominal and soft tissue infections, that can be affecting bloodstream infections. Therefore, since O25b/ST131 has been reported in several studies from Iran, in the current study, we have investigated the molecular characteristics, typing, and biofilm formation of O25b/ST131 clone type E. coli collected from UTI specimens. Methods A total of 173 E. coli isolates from UTI were collected. The susceptibility to all fourth generations of cephalosporins (cefazolin, cefuroxime, ceftriaxone, cefotaxime, ceftazidime, cefepime) and ampicillin, ampicillin-sulbactam and aztreonam was determined. Class A ESBLs, class D ESBL and the presence of pabB gene screenings to detect of O25b/ST131 clone type were performed by using of PCR. Biofilm formation was compared between O25b/ST131 isolates and non-O25b/ST131 isolates. Finally, ERIC-PCR was used for typing of ESBL positive isolates. Results Ninety-four ESBL positive were detected of which 79 of them were O25b/ST131. Antimicrobial susceptibility test data showed that most antibiotics had a higher rate of resistance in isolates of the O25b/ST131 clonal type. Biofilm formation showed that there was a weak association between O25b/ST131 clone type isolates and the level of the biofilm formation. ERIC-PCR results showed that E. coli isolates were genetically diverse and classified into 14 groups. Conclusion Our results demonstrated the importance and high prevalence of E. coli O25b/ST131 among UTI isolates with the ability to spread fast and disseminate antibiotic resistance genes.

show abstract

Uropathogenic Escherichia coli endeavors: an insight into the characteristic features, resistance mechanism, and treatment choice

Arafi,

Hasani,

Sadeghi

et al. 2023

Arch Microbiol

View full text Add to dashboard Cite

Distribution of Pathogenicity Island Markers and H-Antigen Types of Escherichia coli O25b/ST131 Isolates from Patients with Urinary Tract Infection in Iran

Cited by 5 publications

References 64 publications

The genotypic and phenotypic characteristics contributing to high virulence and antibiotics resistance in Escherichia coli O25-B2-ST131 in comparison to non- O25-B2-ST131

The genotypic and phenotypic characteristics contributing to high virulence and antibiotics resistance in Escherichia coli O25-B2-ST131 in comparison to non- O25-B2-ST131

Molecular determination of O25b/ST131 clone type among extended spectrum β-lactamases production Escherichia coli recovering from urinary tract infection isolates

Uropathogenic Escherichia coli endeavors: an insight into the characteristic features, resistance mechanism, and treatment choice

Contact Info

Product

Resources

About