Distribution of omega-6 and omega-3 polyunsaturated fatty acids in the whole rat body and 25 compartments

Abstract

The steady state compositions of omega-6 and omega-3 polyunsaturated fatty acids (PUFA) throughout the various viscera and tissues within the whole body of rats have not previously been described in a comprehensive manner. Dams consumed diets containing 10 wt% fat (15% linoleate and 3% α-linolenate). Male offspring (n=9) at 7-wks of age were euthanized and dissected into 25 compartments. Total lipid fatty acids for each compartment were quantified by GC/FID and summed for the rat whole body; total n-6 PUFA was 12 wt% and total n-3 PUFA was 2.1% of total fatty acids. 18:2n-6 accounted for 84% of the total n-6 PUFA, 20:4n-6 was 12%, 18:3n-3 was 59% of the total n-3 PUFA, 20:5n-3 was 2.1%, and 22:6n-3 was 32%. The white adipose tissue contained the greatest amounts of 18:2n-6 (1.5 g) and 18:3n-3 (0.2 g). 20:4n-6 was highest in muscle (60 mg) and liver (57 mg), while 22:6n-3 was greatest in muscle (46 mg), followed by liver (27 mg) and carcass (20 mg). In terms of fatty acid composition expressed as a percentage, 18:2n-6 was the highest in the heart (13 wt%), while 18:3n-3 was about 1.3 wt% for skin, white adipose tissue and fur. 20:4n-6 was highest (21–25 wt%) in the circulation, kidney, and spleen, while 22:6n-3 was highest in the brain (12 wt%), followed by the heart (7.9 wt%), liver (5.9 wt%), and spinal cord (5.1 wt%). Selectivity was greatest when comparing 22:6n-3 in brain (12%) to white adipose (0.08%) (68-fold) and 22:5n-6 in testes (15.6%) compared to white adipose (0.02%), 780-fold.

Aging dysregulates D- and E-series resolvins to modulate cardiosplenic and cardiorenal network following myocardial infarction

“…In the clinical setting, post-MI injury leads to the development of LV dysfunction, as well as histological and structural changes that account for HF due to reperfusion therapy, but the triggers of this unknown chemical remodeling presented a challenge in the 20 th century [19]. Using lipidomics technology, we measured the AA, DHA, and EPA metabololipidome in young and aging mice in relevance to excess omega-6 intake in a post-MI setting.…”

Interactive effects of dietary lipids and vitamin E level on performance, blood eicosanoids, and response to mitogen stimulation in broiler chickens of different ages

“…Salem et al. (2015) have shown that brain in rats has an apparent selectivity in uptake of FA, in which DHA is predominate. A significant interaction also was documented in the case of DHA level in the brain lipids.…”

Impact of n-3 Docosapentaenoic Acid Supplementation on Fatty Acid Composition in Rat Differs Depending upon Tissues and Is Influenced by the Presence of Dairy Lipids in the Diet

“…Article expressed (liver, kidney, bone marrow), 36 (ii) either tissues rich in mitochondria and energy intensive, the energetic role being the first quantitative use of fatty acids (heart, lung), 37 (iii) either marker tissues of food consumption and fatty acid metabolism of animals (RBC, spleen), 38 or (iv) either tissues exhibiting the highest proportions of n-3 DPA. 39 Despite the relative lack of literature on the subject, our results are of interest when considering the physiological effects reported in these tissues. Concerning the spleen, a major organ involved in inflammation regulation, some experiments showed that n-3 DPA reduced inflammation in in vitro models, 40 associated with the action of some hydroxymetabolites directly derived from n-3 DPA like resolvins.…”