DISTRIBUTION OF LETHALS INDUCED BY TRITIATED DNA PRECURSORS IN <i>DROSOPHILA MELANOGASTER</i>

Kaplan, William D.; Gugler, H. D.; Kidd, K.K.

doi:10.1093/genetics/53.3.499

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1967

1984

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Effect of radioactive amino acids on chromosomes, viability and two‐dimensional protein patterns of cultured mammalian cells

Zeindl

Klose

1984

Electrophoresis

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Effect of radioactive amino acids on chromosomes, viability and two-dimensional protein patterns of cultured mammalian cellsThe effect of 14C-labelled amino acids on three different established mammalian cell lines, i. e., on Hep-cells, HeLa-cells, and Chinese hamster ovary cells, was studied. The cells were incubated in the presence of 20 pCi 14C-amino acids per 1 ml culture medium for 0-24 h or in the presence of 200 pCi/ml for 30 min. Thereafter, the chromosomes of the cells, the ability of the cells to form colonies (plating efficiency) and the protein patterns of the cells obtained by two-dimensional electrophoresis were investigated. The results showed an increase in the number of chromosome breaks and a decrease in the plating efficiency when the incubation period was increased. In contrast, the frequency of qualitative and quantitative changes in the protein patterns of cells exposed to radioactive amino acids was not increased over the control level. Possible reasons for this discrepancy are discussed.

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