Distribution of Ion-Implanted Nitrogen in Iron Alloys Investigated by AES

Baunack, S.; Schreiber, Gerhard; Oettel, H.; Blawert, Carsten; Mordike, B. L.

doi:10.1007/s006040050017

Cited by 53 publications

(35 citation statements)

References 14 publications

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“…41,42 Encapsulation in porous nanoparticles goes a further stage to making reliable intracellular assessments. [43][44][45][46][47] In this paper, we show that two independent approaches, namely pinhole shifting in a two-photon excitation beam and epi-phosphorescence timecorrelated single photon counting (TCSPC), give similar estimates of local O 2 concentrations within several di®erent single-celled organisms and in suspensions of cultured mammalian cells. Some of these data have been published in preliminary form in a \Technical Design Note" note.…”

Section: Introductionmentioning

confidence: 96%

Intracellular oxygen: Similar results from two methods of measurement using phosphorescent nanoparticles

Lloyd

Williams

Vijayalakshmi

et al. 2014

J. Innov. Opt. Health Sci.

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The ability to resolve the spatio-temporal complexity of intracellular O 2 distribution is the \Holy Grail" of cellular physiology. In an e®ort to obtain a minimally invasive approach to the mapping of intracellular O 2 tensions, two methods of phosphorescent lifetime imaging microscopy were compared in the current study and gave similar results. These were two-photon confocal laser scanning microscopy with pinhole shifting, and picosecond time-resolved epi-phosphorescence microscopy using a single 0.5 m focused spot. Both methods utilized Ru coordination complex embedded nanoparticles (45 nm diameter) as the phosphorescent probe, excited using pulsed outputs of a titanium-sapphire Tsunami lasers (710-1050 nm).

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Section: Introductionmentioning

confidence: 96%

Intracellular oxygen: Similar results from two methods of measurement using phosphorescent nanoparticles

Lloyd

Williams

Vijayalakshmi

et al. 2014

J. Innov. Opt. Health Sci.

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“…After being embedded into nanoparticles (NPs), these oxygen probes are isolated from the outside environment by the particle matrix. Therefore, photostability and cell toxicity of probe dyes are greatly improved in NPs [11][12][13][14]. Although oxygen can be sensed by single-intensity-based methods [15][16][17], more robust signals are obtained by ratiometric fluorescence methods [18,19].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of ratiometric fluorescent nanoparticles for sensing oxygen

et al. 2012

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A facile reprecipitation-encapsulation method is used for the preparation of ratiometric fluorescent nanoparticles (NPs) for sensing intracellular oxygen. The surface of the NPs is modified in-situ with poly-L-lysine, which renders good biocompatibility and enables easy internalization into living cells. The sensor NPs contain a red fluorescent probe whose fluorescence is sensitive to oxygen with a quenching response of 77 % on going from nitrogen saturation to oxygen saturation, and a reference dye giving a green signal that acts as an oxygen-independent reference. The ratio of the two emissions serves as the analytical information and is sensitive to dissolved oxygen in the 0-43 ppm concentration range. When incorporated into cells, the ratio of the signals increases by 400 % on going from oxygen-saturated to oxygen-free environment.

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“…Microemulsion templated synthesis has a wide range of applications going from preparation of new types of copolymers [6,7], particles for drug delivery [8], preparation of metal clusters [9,10], to formation of polymer beads with a narrow polydispersity [11,12]. In particular, microemulsion templated synthesis has been exploited to prepare nanosized sensoring devices for use in, e.g., living cells [13].…”

Section: Introductionmentioning

confidence: 99%