Distribution of <i>Dekkera bruxellensis</i>
 in a sugarcane-based fuel ethanol fermentation plant

Silva, T.C.D. da; Leite, Fernanda Cristina Bezerra; Morais, Marcos Antônio de

doi:10.1111/lam.12558

Cited by 12 publications

(5 citation statements)

References 11 publications

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“…We have just reported that D. bruxellensis is introduced in the industrial process at the beginning of the harvest season with the sugarcane and first accumulated in the wash water that is further recirculated over the season (Da Silva et al 2016). Therefore, the heating of this wash water could prevent the input of D. bruxellensis cells from the fields and impede its accumulation in the recycled water.…”

Section: Resultsmentioning

confidence: 99%

High intracellular trehalase activity prevents the storage of trehalose in the yeastDekkera bruxellensis

Leite

Pereira

et al. 2016

Lett Appl Microbiol

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Dekkera bruxellensis can successfully take advantage of its peculiar physiological and genetic traits in order to adapt and survive in fermentation processes. So far, tolerance to stress has been credited to trehalose synthesis. The data presented in this work provided information on the underlying mechanism that prevents trehalose accumulation and corroborated the recent information that trehalose itself is not implicated in yeast stress tolerance. Second, it showed that D. bruxellensis responds differently to Saccharomyces cerevisiae to excess of sugar, which may explain its preference for respiration (oxidative metabolism) over fermentation (reductive metabolism) even at limited oxygen supply. These findings help to understand the drop on ethanol production in processes overtaken by this yeast.

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Section: Resultsmentioning

confidence: 99%

High intracellular trehalase activity prevents the storage of trehalose in the yeastDekkera bruxellensis

Leite

Pereira

et al. 2016

Lett Appl Microbiol

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“…The strains of B. bruxellensis used in the present work were previously isolated from fuel ethanol production processes (Table 1) and identified by molecular biology tools (da Silva, Leite, & de Morais, 2016). Strains were maintained in yeast extract peptone dextrose (YPD) medium (10 g/L yeast extract, 20 g/L glucose, 20 g/L bacteriological peptone and 20 g/L agar) with constant transfers to new Petri dishes in order to keep cell colonies fresh (Leite et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

Fermentation profiles of the yeast Brettanomyces bruxellensis in d‐xylose and l‐arabinose aiming its application as a second‐generation ethanol producer

Silva

Ribeiro

Teles

et al. 2020

Yeast

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The yeast Brettanomyces bruxellensis is able to ferment the main sugars used in firstgeneration ethanol production. However, its employment in this industry is prohibitive because the ethanol productivity reached is significantly lower than the observed for Saccharomyces cerevisiae. On the other hand, a possible application of B. bruxellensis in the second-generation ethanol production has been suggested because this yeast is also able to use D-xylose and L-arabinose, the major pentoses released from lignocellulosic material. Although the latter application seems to be reasonable, it has been poorly explored. Therefore, we aimed to evaluate whether or not different industrial strains of B. bruxellensis are able to ferment D-xylose and Larabinose, both in aerobiosis and oxygen-limited conditions. Three out of nine tested strains were able to assimilate those sugars. When in aerobiosis, B. bruxellensis cells exclusively used them to support biomass formation, and no ethanol was produced. Moreover, whereas L-arabinose was not consumed under oxygen limitation, D-xylose was only slightly used, which resulted in low ethanol yield and productivity. In conclusion, our results showed that D-xylose and L-arabinose are not efficiently converted to ethanol by B. bruxellensis, most likely due to a redox imbalance in the assimilatory pathways of these sugars. Therefore, despite presenting other industrially relevant traits, the employment of B. bruxellensis in second-generation ethanol production depends on the development of genetic engineering strategies to overcome this metabolic bottleneck.

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“…Afterwards, the suspensions were submitted to serial dilutions with 0.85% sterile NaCl 0.9% w/v solution and 100 mL of each dilution were plated on synthetic medium containing 1.7 g/L yeast nitrogen base (YNB) without amino acids and ammonium (Sigma-Aldrich, St. Luis, USA), 20 g/L glucose and 5 g/L ammonium sulphate, supplemented with antibiotics and cycloheximide as above. The plates were incubated for five days at 30 o C. The colonies were transferred for YPD plates (10 g/L yeast extract, 20 g/L peptone, 20 g/L glucose and 20 g/L bacto-agar) and incubated for more five days at 30 o C. Colonies were separated by morphological inspection and checked for purity [23][24][25].…”

Section: Soil Processing and Yeast Isolationmentioning

confidence: 99%

Meyerozyma Caribbica Isolated From Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

Alencar¹,

Freitas²,

Silva³

et al. 2023

Preprint

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The production of fuels and other industrial products from renewable sources has intensified the search for new substrates or for the expansion of the use of substrates already in use, as well as the search for microorganisms with different metabolic capacities. In the present work, we isolated and tested a yeast from the soil of sugarcane irrigated with vinasse, that is, with high mineral content and acidic pH. The strain of Meyerozyma caribbica URM 8365 was able to ferment glucose, but the use of xylose occurred when some oxygenation was provided. However, some fermentation of xylose to ethanol in oxygen limitation also occurs if glucose is present. This strain was able to produce ethanol from molasses substrate with 76% efficiency, showing its tolerance to possible inhibitors. High ethanol production efficiencies were also observed in acidic hydrolysates of each bagasse, sorghum, and cactus pear biomass. Mixtures of these substrates were tested and the best composition was found for the use of excess plant biomass in supplementation of primary substrates. It was also possible to verify the production of xylitol from xylose when the acetic acid concentration is reduced. Finally, the proposed metabolic model allowed calculating how much of the xylose carbon can be directed to the production of ethanol and/or xylitol in the presence of glucose. With this, it is possible to design an industrial plant that combines the production of ethanol and/or xylitol using combinations of primary substrates with hydrolysates of their biomass.

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Distribution of Dekkera bruxellensis in a sugarcane-based fuel ethanol fermentation plant

Cited by 12 publications

References 11 publications

High intracellular trehalase activity prevents the storage of trehalose in the yeastDekkera bruxellensis

High intracellular trehalase activity prevents the storage of trehalose in the yeastDekkera bruxellensis

Fermentation profiles of the yeast Brettanomyces bruxellensis in d‐xylose and l‐arabinose aiming its application as a second‐generation ethanol producer

Meyerozyma Caribbica Isolated From Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

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