High intracellular trehalase activity prevents the storage of trehalose in the yeast<i>Dekkera bruxellensis</i>

Leite, Fernanda Cristina Bezerra; Leite, Danilo; Pereira, Leonardo Costa; Pita, Will de Barros; Morais, Marcos Antônio de

doi:10.1111/lam.12609

Cited by 12 publications

(5 citation statements)

References 19 publications

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“…bruxellensis (Leite, Leite Dvda, Pereira, et al, 2016). As hypothesized above, this result is the confirmation that the inactivation of ACDH relieves (or partially relieves) the repressive activity of GCR mechanism.…”

Section: Was 27%supporting

confidence: 79%

“…Unexpectedly, an increase was observed in the transcript level of the gluconeogenic gene FBP1, by 50‐fold (Figure b). The products of these genes work in opposite directions and the second is subjected to GCR also in D. bruxellensis (Leite, Leite Dvda, Pereira, et al, ). As hypothesized above, this result is the confirmation that the inactivation of ACDH relieves (or partially relieves) the repressive activity of GCR mechanism.…”

Section: Resultsmentioning

confidence: 99%

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First aspects on acetate metabolism in the yeast Dekkera bruxellensis: a few keys for improving ethanol fermentation

Teles

Silva

Mendonça

et al. 2018

Yeast

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Dekkera bruxellensis is continuously changing its status in fermentation processes, ranging from a contaminant or spoiling yeast to a microorganism with potential to produce metabolites of biotechnological interest. In spite of that, several major aspects of its physiology are still poorly understood. As an acetogenic yeast, minimal oxygen concentrations are able to drive glucose assimilation to oxidative metabolism, in order to produce biomass and acetate, with consequent low yield in ethanol. In the present study, we used disulfiram to inhibit acetaldehyde dehydrogenase activity to evaluate the influence of cytosolic acetate on cell metabolism. D. bruxellensis was more tolerant to disulfiram than Saccharomyces cerevisiae and the use of different carbon sources revealed that the former yeast might be able to export acetate (or acetyl-CoA) from mitochondria to cytoplasm. Fermentation assays showed that acetaldehyde dehydrogenase inhibition re-oriented yeast central metabolism to increase ethanol production and decrease biomass formation. However, glucose uptake was reduced, which ultimately represents economical loss to the fermentation process. This might be the major challenge for future metabolic engineering enterprises on this yeast.

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Section: Was 27%supporting

confidence: 79%

Section: Resultsmentioning

confidence: 99%

First aspects on acetate metabolism in the yeast Dekkera bruxellensis: a few keys for improving ethanol fermentation

Teles

Silva

Mendonça

et al. 2018

Yeast

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“…Finally, all strains started consuming d ‐xylose before the complete exhaustion of glucose in oxygen‐limited conditions (Figure 4). It seems that glucose repression is less strict in our strains than it is in S. cerevisiae , as it was previously reported (da Silva et al, 2019; Leite, Leite, Pereira, de Barros, & de Morais, 2016), allowing both sugars to be co‐consumed. This is an interesting result because the co‐utilization of glucose and d ‐xylose is not common, but it is essential for the conversion of lignocellulose to ethanol to be economically viable (Rech et al, 2019).…”

Section: Resultssupporting

confidence: 66%

Fermentation profiles of the yeast Brettanomyces bruxellensis in d‐xylose and l‐arabinose aiming its application as a second‐generation ethanol producer

Silva

Ribeiro

Teles

et al. 2020

Yeast

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The yeast Brettanomyces bruxellensis is able to ferment the main sugars used in firstgeneration ethanol production. However, its employment in this industry is prohibitive because the ethanol productivity reached is significantly lower than the observed for Saccharomyces cerevisiae. On the other hand, a possible application of B. bruxellensis in the second-generation ethanol production has been suggested because this yeast is also able to use D-xylose and L-arabinose, the major pentoses released from lignocellulosic material. Although the latter application seems to be reasonable, it has been poorly explored. Therefore, we aimed to evaluate whether or not different industrial strains of B. bruxellensis are able to ferment D-xylose and Larabinose, both in aerobiosis and oxygen-limited conditions. Three out of nine tested strains were able to assimilate those sugars. When in aerobiosis, B. bruxellensis cells exclusively used them to support biomass formation, and no ethanol was produced. Moreover, whereas L-arabinose was not consumed under oxygen limitation, D-xylose was only slightly used, which resulted in low ethanol yield and productivity. In conclusion, our results showed that D-xylose and L-arabinose are not efficiently converted to ethanol by B. bruxellensis, most likely due to a redox imbalance in the assimilatory pathways of these sugars. Therefore, despite presenting other industrially relevant traits, the employment of B. bruxellensis in second-generation ethanol production depends on the development of genetic engineering strategies to overcome this metabolic bottleneck.

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“…These substances play important roles in strain defense system. Trehalose metabolism ( TPS2 , TSL1 , and ATH1 ) has been found to protect cell biomacromolecules from stress effects caused by acetic acid, high temperature, and high osmotic pressure [ 86 , 89 ]. Glucan synthesis ( FKS1 , FKS2 , and ROM2 ) has been found to involve in cell wall remodeling under acetic acid stress [ 90 , 91 ].…”

Section: Response Mechanisms Of S Cerevisiae To St...mentioning

confidence: 99%

Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains

Liu

Zhao

2022

Biotechnol Biofuels

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Bioconversion of lignocellulosic biomass to biofuels such as bioethanol and high value-added products has attracted great interest in recent decades due to the carbon neutral nature of biomass feedstock. However, there are still many key technical difficulties for the industrial application of biomass bioconversion processes. One of the challenges associated with the microorganism Saccharomyces cerevisiae that is usually used for bioethanol production refers to the inhibition of the yeast by various stress factors. These inhibitive effects seriously restrict the growth and fermentation performance of the strains, resulting in reduced bioethanol production efficiency. Therefore, improving the stress response ability of the strains is of great significance for industrial production of bioethanol. In this article, the response mechanisms of S. cerevisiae to various hydrolysate-derived stress factors including organic acids, furan aldehydes, and phenolic compounds have been reviewed. Organic acids mainly stimulate cells to induce intracellular acidification, furan aldehydes mainly break the intracellular redox balance, and phenolic compounds have a greater effect on membrane homeostasis. These damages lead to inadequate intracellular energy supply and dysregulation of transcription and translation processes, and then activate a series of stress responses. The regulation mechanisms of S. cerevisiae in response to these stress factors are discussed with regard to the cell wall/membrane, energy, amino acids, transcriptional and translational, and redox regulation. The reported key target genes and transcription factors that contribute to the improvement of the strain performance are summarized. Furthermore, the genetic engineering strategies of constructing multilevel defense and eliminating stress effects are discussed in order to provide technical strategies for robust strain construction. It is recommended that robust S. cerevisiae can be constructed with the intervention of metabolic regulation based on the specific stress responses. Rational design with multilevel gene control and intensification of key enzymes can provide good strategies for construction of robust strains. Graphical Abstract

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High intracellular trehalase activity prevents the storage of trehalose in the yeastDekkera bruxellensis

Cited by 12 publications

References 19 publications

First aspects on acetate metabolism in the yeast Dekkera bruxellensis: a few keys for improving ethanol fermentation

First aspects on acetate metabolism in the yeast Dekkera bruxellensis: a few keys for improving ethanol fermentation

Fermentation profiles of the yeast Brettanomyces bruxellensis in d‐xylose and l‐arabinose aiming its application as a second‐generation ethanol producer

Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains

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