Distribution of human papillomavirus types in genital lesions from two temporally distinct populations determined by in situ hybridization

Anderson, Steven M.; Brooke, Peter; Eyck, S.L. Van

doi:10.1016/0046-8177(93)90168-g

Cited by 9 publications

(6 citation statements)

References 26 publications

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“…The HPV type distribution described in this study is in agreement with several other reports [4,25,45,57]. No specific relationships were observed between the density of intraepithelial Langerhans' cells or stromal macrophages and the HPV types detected in SILs separated into low and high grade.…”

Section: Discussionsupporting

confidence: 95%

Inverse modulation of intraepithelial Langerhans' cells and stromal macrophage/dendrocyte populations in human papillomavirus-associated squamous intraepithelial lesions of the cervix

Al-Saleh

Delvenne

Arrese

et al. 1995

Vichows Archiv A Pathol Anat

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Ninety-four cervical biopsies from normal tissue to high-grade squamous intraepithelial lesion (SILs) were examined for the presence of intraepithelial Langerhans' cells and subpopulations of stromal macrophages/dendrocytes by immunohistochemistry using anti-S100, -L1, -CD68 and -factor XIIIa antibodies. Human papillomavirus (HPV) detection was performed in all cases by using first a mixture of DNA probes for 14 HPV types commonly found in anogenital biopsies at low stringency conditions (Tm -40 degrees C) and by reanalyzing the tissues at high stringency (Tm -10 degrees C) with HPV 6/11, 16/18 and 31/33/35 biotinylated probe cocktails and individual digoxigenin-labelled probes. SILs and metaplastic tissues were significantly associated with a depletion of S100-positive intraepithelial Langerhans' cells when compared with normal epithelium. In contrast, there was a significant increase in L1-positive stromal macrophages in SIL biopsies compared with normal or metaplastic cervix. A significantly higher density of CD68-positive macrophages was also observed in high-grade SILs compared with normal or metaplastic biopsies and with low-grade SILs. The density of factor XIIIa-positive dendrocytes was found to be higher in SILs compared with metaplastic tissues and in high-grade SILs when compared with normal cervical biopsies. No specific relationship was found between the densities of these cells and the HPV type detected in SILs separated into low grade and high grade. The significance of this inverse modulation of intraepithelial Langerhans' cells and stromal macrophages/dendrocytes in normal and SIL biopsies is discussed in relation to HPV infection and malignant transformation.

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Section: Discussionsupporting

confidence: 95%

Inverse modulation of intraepithelial Langerhans' cells and stromal macrophage/dendrocyte populations in human papillomavirus-associated squamous intraepithelial lesions of the cervix

Al-Saleh

Delvenne

Arrese

et al. 1995

Vichows Archiv A Pathol Anat

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“…However, the technique has given some insight into the epidemiology, in that similar patterns of infections were identified in two cohorts separated by 25 years. 156 ISH may be a useful adjunct in the investigation of HPV infection of the male genitalia. 157,158 The role played by HPV in other lesions, including Bowen's disease, 159 squamous cell papilloma of bronchus, 160 and larynx, 161 is unclear.…”

Section: Infectionsmentioning

confidence: 99%

In Situ hybridization and its diagnostic applications in pathology

1997

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In situ hybridization (ISH) is a technique by which specific nucleotide sequences are identified in cells or tissue sections. These may be endogenous, bacterial or viral, DNA or RNA. On the basis of research applications, the technique is now being translated into diagnostic practice, mainly in the areas of gene expression, infection and interphase cytogenetics. Diagnostic applications are most often based on short nucleotide sequences (oligomers) labelled with non‐isotopic reporter molecules, and sites of binding may be localized by histochemical or immunohistochemical methods. The technique can be applied to routinely fixed and processed tissues; with some targets, it is even possible to obtain hybridization in autopsy material. ISH has been used to detect messenger RNA (mRNA) as a marker of gene expression, where levels of protein storage are low; for example, to confirm an endocrine tumour as the source of excess hormone production. Its application in infectious diseases has to date been mainly in viral infections, such as the typing of human papillomavirus (HPV) or the detection of Epstein–Barr virus by the presence of small nuclear RNAs (EBERs). The expression of mRNAs for histone proteins has been used to detect cells in S phase, and related methods may be applied to detect apoptotic cells. Using probes to chromosome‐specific sequences, it is possible to detect aneuploidy, and to document changes in specific chromosomes, which may have prognostic significance in some tumours, such as B‐cell chronic lymphatic leukaemia. Using sequence‐specific probes, translocations can be identified, such as the t(11;12) of Ewing's sarcoma. This review presents an outline of the technique of in situ hybridization and discusses areas of current and potential diagnostic application. © 1997 John Wiley & Sons, Ltd.

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“…Entre os casos com LIE-BG, 41,9% eram positivos para HPV. Este resultado é próximo ao mencionado por Anderson et al 12 (52%) e abaixo do encontrado por Nuovo et al 13 (72%), Richart e Nuovo 14 (91%), Gómez et al 10 (65,4%) e Cavalcanti et al 15 (85,6%). A baixa sensibilidade da HIS explica os baixos percentuais encontrados por nós nas LIE-BG, sabidamente causadas por HPV, nas quais o padrão epissomal de DNA de HPV deveria ter sido detectado mais vezes.…”

Section: Discussionunclassified

“…Entre os casos com LIE-AG, 62,3% eram positivos, cifra pouco inferior à encontrada por Fallani et al 16 (80%), Gómez et al 10 (78,4%) e Anderson et al 12…”

Section: Discussionunclassified

Tipagem e estado físico de papilomavírus humano por hibridização in situ em lesões intra-epiteliais do colo uterino

Bagarelli¹,

Oliani²

2004

Rev. Bras. Ginecol. Obstet.

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Distribution of human papillomavirus types in genital lesions from two temporally distinct populations determined by in situ hybridization

Cited by 9 publications

References 26 publications

Inverse modulation of intraepithelial Langerhans' cells and stromal macrophage/dendrocyte populations in human papillomavirus-associated squamous intraepithelial lesions of the cervix

Inverse modulation of intraepithelial Langerhans' cells and stromal macrophage/dendrocyte populations in human papillomavirus-associated squamous intraepithelial lesions of the cervix

In Situ hybridization and its diagnostic applications in pathology

Tipagem e estado físico de papilomavírus humano por hibridização in situ em lesões intra-epiteliais do colo uterino

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