Distribution of Human Papillomavirus Types in Women from Valencia, Spain, with Abnormal Cytology

García-García, José Ángel; Pérez‐Vallés, Ana; Martorell, Miguel; Gómez, Beatriz; Gómez-Cabrero, David; Soler, Francesc; Calabuig, Consuelo

doi:10.1159/000325001

Cited by 13 publications

(14 citation statements)

References 21 publications

(19 reference statements)

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“…Multiple HPV infections decrease with severity of lesion, being less frequent in HSIL [34], a fact that coincides with the present findings. However, in a study of 298 women with abnormal cervical cytology in Kuwait [27], reported that as the severity of the cytological diagnosis increased the proportion of single infections decreased, while the proportion of multiple infections increased, and Fife et al [39] found 85.3% of multiple HPV infections in dysplasia and 61.9% in ASC-US, these last concluding that their data support a possible role for multiple HPV types in the development or progression of cervical dysplasia.…”

Section: Discussionsupporting

confidence: 92%

“…The distribution of HPV genotypes in this study was consistent with that reported in studies previously [20,29,30,34]. HPV16 has been consistently identified as the most frequent HR-HPV.…”

Section: Discussionsupporting

confidence: 91%

“…Our results fall within this range. This prevalence of multiple HPV infection is higher than found in other studies conducted in Spain in which the values range between 4.7% and 52.1% [6,29,30,34,36,38,57]. Furthermore, in a study of patients with abnormal cytology obtained a higher value (58.1%) [35] than found in this study.…”

Section: Discussioncontrasting

confidence: 79%

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Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Méndez¹

2013

SOJ Microbiol Infect Dis

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BackgroundCervical cancer is the second most common cancer in women worldwide and the most common cause of mortality in underdeveloped and developing countries [1,2]. Persistent infection with certain oncogenic high-risk (HR) types of Human Papillomavirus (HPV) is the required factor for the development of invasive cervical cancer and precursor lesions [3][4][5][6].Overall, the prevalence and distribution of HPV infection in cervical lesions has been determined in many geographical regions of the world, this can vary depending on several factors including epidemiological differences in the populations studied and the methodology used for molecular detection and typing of HPV DNA [7]. Over 120 types of HPV have been classified as either low (LR-HPV) or high risk (HR-HPV), according to their oncogenic potential [8,9]. Within the high-risk genotypes, HPV types 16 and HPV 18 are most often associated with cancer and squamous intraepithelial lesion [5,6,10,11,12]. Nevertheless, the risk of neoplasia for other types of HPV as well as for multiple HPV infection has not yet been established.Because current strategies for the prevention of cervical cancer and their precancerous lesions are based on the HPV genotyping [13] and prophylactic vaccines [14][15][16], it seems necessary to determine the types of HPV most commonly associated with malignant cell transformation in different geographical areas in order to establish effective preventive measures according to the epidemiology of the population. This study was to know the prevalence and distribution of cervical HPV infection in women attending routine cervical cancer screening in the Valencia region (Spain). Study designLiquid-based cytology (LBC) of cervical specimens was collected during routine screening visits, between June 20, 2011 and September 9, 2011 in the Clinical Hospital of Valencia, Spain. LBC samples were processed using the Thin Prep® 5000 processor (Madrid, Spain). For HPV detection and genotyping, we used the residual cervical sample material available from the same Thin Prep Pap test vial previously used for cytological screening. DNA was extracted from the residual materials of LBC by QIAamp® DNA mini kit (IZASA, Valencia, Spain), according to the manufacturer's instructions. HPV testing was performed by INNO-Lipa HPV genotyping Extra Reverse Hybridization Line Probe Assay kit (Innogenetics®, Barcelona, Spain), according to the manufacturer's instructions. Briefly, HPV detection and genotyping was based on PCR amplification of a 65pb fragment, within the L1 region of the HPV genome, using broad-spectrum SPF10 biotinylated primers. PCR was performed in a final AbstractBackground: Infection with human papillomavirus (HPV) has been identified as the primary cause of cervical cancer. Persistent infection with high risk-HPV is the required factor for the development of cervical cancer.

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Section: Discussionsupporting

confidence: 92%

“…The distribution of HPV genotypes in this study was consistent with that reported in studies previously [20,29,30,34]. HPV16 has been consistently identified as the most frequent HR-HPV.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Méndez¹

2013

SOJ Microbiol Infect Dis

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“…HPV genotyping has been used in gynecologic cytopathology in the past few years [4,5] but only recently is emerging as an important tool in the management of patients with OPC. Most publications in the literature are based on HPV 16 detection in surgical pathology specimens and use HPV detection methods such as PCR and ISH [6,7,8,9,10].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Fine Needle Aspiration Specimen Adequacy for High-Risk HPV Detection and Genotyping in Oropharyngeal Squamous Cell Carcinoma

Solomides¹,

Bibbo²,

Wang³

2012

Acta Cytologica

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Objective: The aim of this study was to determine the adequacy of archived and fresh fine needle aspiration (FNA) specimens from metastatic head and neck squamous cell carcinoma (SCC) for the molecular detection and genotyping of high-risk (HR) HPV. Study Design: Thirty-seven specimens from 26 patients diagnosed by FNA with metastatic SCC were included as retrospective specimens [19 slides stained with Papanicolaou (Pap) and 18 with Diff-Quik® (DQ)]. Twenty fresh FNA specimens from 18 patients were included as prospective specimens. These specimens were analyzed using the standard protocol for ThinPrep® cervical specimens, with a Cervista HR HPV detection kit. The positive specimens were tested for the HPV 16 and 18 genotypes. Results: Forty-four of 57 specimens (77%) had sufficient cells to yield a valid HPV result. The adequacy rate for Pap-stained slides was 15/19 (79%), for DQ-stained slides it was 13/18 (72%), and for fresh needle aspirates it was 16/20 (80%). HR HPV was detected in 23/44 (52%) specimens. Among the 23 HPV-positive specimens, 19 were genotyped as HPV 16 and 1 as HPV 18. Conclusions: HR HPV detection and genotyping can be performed on FNA specimens of head and neck SCC prospectively collected in PreservCyt as well as on archival slides with either Pap or DQ stain.

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“…This evidence shows the higher impact of HPV16 (prevalence 20.6% in Iq) within well-communicated populations. HPV16 has been targeted by vaccine, which was recently implemented and is considered one of the HPV types with higher risk of associated carcinoma Garcia-García et al, 2010). However, the prevalence of HPV16 is much higher that the prevalence found in developed countries.…”

Section: Discussionmentioning

confidence: 99%

Short Communication Comparison of the prevalence and distribution of human papillomavirus infection and cervical lesions between urban and native habitants of an Amazonian region of Peru

Martorell¹,

García-García²,

Gomez-Cabrero³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. We made a study of the prevalence of human papillomavirus (HPV) and cervix lesions in an Amazonian Bora native population (Bn) and compared it with the prevalence in an urban population in Iquitos (Iq). We also examined the distribution of HPV types among abnormal cervical smears in the Iq population. Swabs and cytologies were collected from 472 females. DNA consensus PCR, followed by direct sequencing, were used to determinate the HPV types in the swabs. Cytologies were classified based on the lesion grade. HPV prevalence was 43.9% in Iq and 35.4% in Bn. Cervix lesion prevalence was 20.0% in Iq and 0.3% in Bn. The frequency of high-risk HPV types among HPV+ females was 71.9% in Iq and 56.3% in Bn. The frequencies of low-risk and undetermined risk HPV types were 19.3/6.3% (Iq/ Bn) and 12.3/37.5% (Iq/Bn), respectively. In lesIq (women seeking cytological services with a previous cervix lesion diagnosis) the prevalence of HPV, was 72.9%. The incidence of carcinoma and high-grade squamous intraepithelial cervix lesions in lesIq were 31.2 and 18.8%, respectively. The Bn population had a lower incidence of high-risk HPV and cervix lesions. The high-risk strain HPV16 was significantly more frequent in Iq compared with Bn. lesIq high-risk HPVs were more frequent in high-grade squamous intraepithelial lesions and carcinoma. High-risk HPV16 prevalence was significantly higher than the prevalence of the other high-risk HPVs, especially in the high-grade squamous intraepithelial lesions and carcinoma.

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Distribution of Human Papillomavirus Types in Women from Valencia, Spain, with Abnormal Cytology

Cited by 13 publications

References 21 publications

Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Prevalence of Human Papillomavirus (HPV) Genotypes and Multiple Infections in Routine Cervical Cancer Screening in a Spanish Regional Population

Assessment of Fine Needle Aspiration Specimen Adequacy for High-Risk HPV Detection and Genotyping in Oropharyngeal Squamous Cell Carcinoma

Short Communication Comparison of the prevalence and distribution of human papillomavirus infection and cervical lesions between urban and native habitants of an Amazonian region of Peru

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