Distribution of Hepatitis C Virus Genotypes in Istanbul, Turkey

Karabulut, Nuran; Alaçam, Sema; Yolcu, Ayfer; Önel, Mustafa; Ağaçfidan, Ali

doi:10.4103/ijmm.ijmm_17_381

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…Genotype analysis was performed on 124 of 216 patients who were found to be HCV RNA positive; 82% of the cases were determined to be Genotype-1, 13% were Genotype-3, and the remaining 5% were other genotypes and mixed genotypes. The genotype distribution of our study was in line with other studies conducted in Turkey (11). In our study, 70.2% of the patients were found to have a false positive result.…”

Section: Discussionsupporting

confidence: 92%

Updating the Signal-to-cutoff Level to Reduce Anti-hepatitis C Virus False Positivity

Öztürk

Ağalar

2021

Jundishapur J Microbiol

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Background: Anti-hepatitis C virus (anti-HCV) is the only screening test being used in the diagnosis of hepatitis C. In this study, we examined anti-HCV positivity rates in our hospital. Objectives: The aim of administering the anti-HCV test was to distinguish patients with hepatitis C infection from false positivity in patients with reactive results. Methods: The anti-HCV tests were performed at Fatih Sultan Mehmet Training and Research Hospital in Istanbul, Turkey, between January 1, 2015 and December 31, 2019. The patients were evaluated retrospectively in terms of age, gender, anti-HCV titer, the clinic for which the examination was requested, the reason for the examination, and the history of hepatitis C. Results: In this study, 511 patients who had two negative polymerase chain reaction (PCR) results were evaluated as false positive cases and enrolled. The cut-off value was found to be 7.5 IU/ml, with the highest sensitivity of 94.4% and specificity of 94.5% (area under the curve [AUC]: 0.982). The lowest anti-HCV titer (5.2) was from patients without acute hepatitis, who were HCV-RNA positive and diagnosed with chronic hepatitis C. Conclusions: It may be more appropriate to report anti-HCV cut-off value of 0 - 5 as negative, 5 - 7.5 as borderline, and > 7.5 as positive. Working with a more acceptable cut-off level with a greater number of tests can help identify patients with asymptomatic HCV infection. Also, it can possibly reduce the cost due to a decrease in the number of PCR tests administered.

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Section: Discussionsupporting

confidence: 92%

Updating the Signal-to-cutoff Level to Reduce Anti-hepatitis C Virus False Positivity

Öztürk

Ağalar

2021

Jundishapur J Microbiol

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“…14,,22 Yaşlı hastalarda genotip 1 ve genç hastalarda genotip 3'ün daha yaygın olduğu tespit edilmiştir. 22 İstanbul'da yapılan bir çalışmada; genotip 1a ve genotip 3 erkeklerde, 1b ise kadınlarda daha yüksek saptanmıştır. 23 25 Kahramanmaraş'ta yapılan çalışmada da, genotiplere göre olguların HCV-RNA dağılımları arasında istatistiksel anlamlı bir ilişki bulunamamıştır.…”

Section: Discussionunclassified

Evaluation of Hepatitis C virus genotype and viral loads in patients with chronic Hepatitis C infection: A five-year, single-center retrospective study

BOZOK

Çelik²

2023

Mersin Üniversitesi Sağlık Bilimleri Dergisi

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Amaç: Hepatit C virüsü nedenli kronik hepatitlerin prognozunda ve tedavisinde HCV genotipleri farklılık göstermektedir. Bu çalışmada; Mersin Şehir Eğitim ve Araştırma Hastanesi Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Bölümü’nde, kronik HCV enfeksiyonu tanısı ile takip edilen hastaların HCV genotip ve HCV-RNA değerlerinin değerlendirilmesi amaçlandı. Yöntem: Şubat 2017- Şubat 2022 tarihleri arasında Mersin Şehir Eğitim ve Araştırma Hastanesi Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Bölümü’nde kronik HCV enfeksiyonu tanısı ile takip edilen erişkin 202 hastanın yaş, cinsiyet, HCV-RNA ve genotip verileri hasta dosyaları ve hastane bilgi sistemi aracılığıyla retrospektif olarak değerlendirildi. HCV-RNA değerleri Bosphore Ultra HCV Quantification Detection Kiti ile HCV genotipleri Bosphore HCV Genotyping Kit v3 ile tespit edildi. Bulgular: Beş yıllık dönemde kronik HCV enfeksiyonu tanısı ile takip edilen erişkin 202 hastanın 60’ı (%29.7) kadın, 142’si (%70.3) erkekti. Yaş ortalaması 44.6±18.3 yıldı. Hastaların 65’inde (%32.1) genotip 1b, 53’ünde (%26.2) genotip 1a, 41’inde (%20.3) genotip 3, 24’ünde (%11.9) genotip 4, 11’inde (%5.4) genotip 2, 2’sinde (%1) genotip 5, 2’sinde (%1) genotip 3-4, 2’sinde (%1) genotip 1b-4, 1’inde (%0.5) genotip 2-3, 1’inde (%0.5) genotip 3-4 birlikteliği tespit edildi. 25 (%12.4) yabancı uyruklu hastada genotip 4 anlamlı derecede daha fazla tespit edildi (p<0.001). Ortanca HCV-RNA değeri 167.650 IU/ml (aralık: 105-19.526.197 IU/ml)’ydi. Genotipler arasında HCV-RNA düzeyleri açısından anlamlı bir ilişki saptanmadı. Kadınlarda genotip 1b anlamlı olarak daha fazla saptandı (p<0.05). Genotip 1’i olan hastaların yaş ortalaması diğer genotiplere göre daha yüksekti (48.5 yıl/39.4 yıl, p=0.003). Sonuç: Bölgemizde yabancı uyruklu hasta popülasyonunun son yıllarda artması nedeniyle, bölgemizde daha az görülen genotipler ve mikst tip HCV enfeksiyonlarında artış olması dikkat çekmektedir. HCV genotiplerinin belirlenmesinin, kronik HCV enfeksiyonlarının seyrinde ve tedavisinde yol göstermesi ayrıca bölgedeki HCV epidemiyolojisindeki değişiklikleri göstermesi açısından önemli olduğu düşünülmektedir.

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“…A few target regions of the virus genome can be used to differentiate the genotypes, including the 5'-UTR, core/E1 and NS5B regions. The genotyping analysis on E1, core, and NS5B regions is usually sufficient to determine genotypes and subtypes of the virus as they are relatively well-conserved (25). The conserved nature of the 5'-UTR has made it among the primary target for pan-genomic HCV RNA detection assays and sequence analysis of amplicons to genotype HCV in a clinical laboratory setting.…”

Section: Hcv Genome Organization and Functionmentioning

confidence: 99%

Hepatitis C: A Review on Current and Emerging Genotyping Assays

Mohd Amin,

Mat Jusoh,

Irekeola

et al. 2023

MJMHS

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Hepatitis C is a global public health concern that infects millions of people worldwide. The continual discovery of new genotypes and subtypes of hepatitis C virus (HCV) is an indication of a persistent molecular evolution of the virus. This remains a concern in the efforts towards hepatitis C elimination, as effective management of the disease is, in part, dependent on the HCV genotype responsible for the infection. Accurate HCV screening and quantification using rapid but highly sensitive and reliable methods are crucial for the diagnosis and subsequent management of HCV-related diseases. Thus, this article discusses HCV and the common methods employed for HCV detection and genotyping. While nucleotide sequencing and phylogenetic analysis of core/E1 and NS5B region are regarded as the gold standard and the most recommended method used for HCV genotyping, electrochemical sensors are being explored for their rapidity.

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Distribution of Hepatitis C Virus Genotypes in Istanbul, Turkey

Abstract: The majority of patients with HCV infection had genotype 1 (82.5%), followed by genotype 3, 2 and 4. Monitoring the change in HCV genotype distribution is critical for the development of effective strategies for HCV elimination.

Cited by 7 publications

References 19 publications

Updating the Signal-to-cutoff Level to Reduce Anti-hepatitis C Virus False Positivity

Updating the Signal-to-cutoff Level to Reduce Anti-hepatitis C Virus False Positivity

Evaluation of Hepatitis C virus genotype and viral loads in patients with chronic Hepatitis C infection: A five-year, single-center retrospective study

Hepatitis C: A Review on Current and Emerging Genotyping Assays

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