Distribution of Graphene Oxide and TiO2-Graphene Oxide Composite in A549 Cells

Jin, Chan; Wang, Fude; Tang, Ying; Zhang, Xiangzhi; Wang, Jianqiang; Yang, Yue

doi:10.1007/s12011-014-0027-3

Cited by 47 publications

(38 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytotoxicity of 100 nm GO (125 µg/ml) was also observed in a later study [Ali‐Boucetta et al, ]. By contrast, a single study reported no cytotoxicity for 300 nm GO (100 and 300 µg/ml) [Jin et al, ].…”

Section: Discussionmentioning

confidence: 59%

See 1 more Smart Citation

No cytotoxicity or genotoxicity of graphene and graphene oxide in murine lung epithelial FE1 cells in vitro

Bengtson

Kling

Madsen

et al. 2016

Environ and Mol Mutagen

View full text Add to dashboard Cite

Graphene and graphene oxide receive much attention these years, because they add attractive properties to a wide range of applications and products. Several studies have shown toxicological effects of other carbon‐based nanomaterials such as carbon black nanoparticles and carbon nanotubes in vitro and in vivo. Here, we report in‐depth physicochemical characterization of three commercial graphene materials, one graphene oxide (GO) and two reduced graphene oxides (rGO) and assess cytotoxicity and genotoxicity in the murine lung epithelial cell line FE1. The studied GO and rGO mainly consisted of 2–3 graphene layers with lateral sizes of 1–2 µm. GO had almost equimolar content of C, O, and H while the two rGO materials had lower contents of oxygen with C/O and C/H ratios of 8 and 12.8, respectively. All materials had low levels of endotoxin and low levels of inorganic impurities, which were mainly sulphur, manganese, and silicon. GO generated more ROS than the two rGO materials, but none of the graphene materials influenced cytotoxicity in terms of cell viability and cell proliferation after 24 hr. Furthermore, no genotoxicity was observed using the alkaline comet assay following 3 or 24 hr of exposure. We demonstrate that chemically pure, few‐layered GO and rGO with comparable lateral size (> 1 µm) do not induce significant cytotoxicity or genotoxicity in FE1 cells at relatively high doses (5–200 µg/ml). Environ. Mol. Mutagen. 57:469–482, 2016. © 2016 The Authors. Environmental and Molecular Mutagenesis Published by Wiley Periodicals, Inc.

show abstract

Section: Discussionmentioning

confidence: 59%

“…Cytotoxicity of 100 nm GO (125 mg/ml) was also observed in a later study [Ali-Boucetta et al, 2013]. By contrast, a single study reported no cytotoxicity for 300 nm GO (100 and 300 mg/ml) [Jin et al, 2014]. Cytotoxicity has also been reported in human lung fibroblasts and b-lymphocytes cells after exposure to 100-200 nm GO Wang et al, 2014].…”

Section: Discussionmentioning

confidence: 66%

No cytotoxicity or genotoxicity of graphene and graphene oxide in murine lung epithelial FE1 cells in vitro

Bengtson

Kling

Madsen

et al. 2016

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…For instance, rGO bonded to cell membranes, stimulated receptors and activated mitochondrial pathways, inducing apoptosis [110, 111, 144]. Limited evidence showed that GO is smaller and less toxic than rGO because of the high oxygen content, smoother edges, and hydrophilic properties of the former species [104, 145, 146]. Because of the different surface oxidation states of GO and rGO, GO possessing distinct hydrophilicity might be internalized and taken up by HepG2 cells easily.…”

Section: Origins Of Gfns Toxicitymentioning

confidence: 99%

Toxicity of graphene-family nanoparticles: a general review of the origins and mechanisms

et al. 2016

View full text Add to dashboard Cite

Due to their unique physicochemical properties, graphene-family nanomaterials (GFNs) are widely used in many fields, especially in biomedical applications. Currently, many studies have investigated the biocompatibility and toxicity of GFNs in vivo and in intro. Generally, GFNs may exert different degrees of toxicity in animals or cell models by following with different administration routes and penetrating through physiological barriers, subsequently being distributed in tissues or located in cells, eventually being excreted out of the bodies. This review collects studies on the toxic effects of GFNs in several organs and cell models. We also point out that various factors determine the toxicity of GFNs including the lateral size, surface structure, functionalization, charge, impurities, aggregations, and corona effect ect. In addition, several typical mechanisms underlying GFN toxicity have been revealed, for instance, physical destruction, oxidative stress, DNA damage, inflammatory response, apoptosis, autophagy, and necrosis. In these mechanisms, (toll-like receptors-) TLR-, transforming growth factor β- (TGF-β-) and tumor necrosis factor-alpha (TNF-α) dependent-pathways are involved in the signalling pathway network, and oxidative stress plays a crucial role in these pathways. In this review, we summarize the available information on regulating factors and the mechanisms of GFNs toxicity, and propose some challenges and suggestions for further investigations of GFNs, with the aim of completing the toxicology mechanisms, and providing suggestions to improve the biological safety of GFNs and facilitate their wide application.

show abstract

“…Jin et al demonstrated that rGO/TiO 2 NPs would separate independently into TiO 2 NPs and GO after entering A549 cells. The rGO/TiO 2 NPs composite could induce cytotoxicity in A549 due to the generation of oxidative stress [336]. More recently, Prakash et al prepared rGO (10-50%)/TiO 2 nanocomposites using hydrothermal synthesis, and studied their toxicity in zebrafish embryos and larvae [337].…”

Section: Rgo-modified Tio 2 Npsmentioning

confidence: 99%

Visible-Light Active Titanium Dioxide Nanomaterials with Bactericidal Properties

2020

View full text Add to dashboard Cite

This article provides an overview of current research into the development, synthesis, photocatalytic bacterial activity, biocompatibility and cytotoxic properties of various visible-light active titanium dioxide (TiO2) nanoparticles (NPs) and their nanocomposites. To achieve antibacterial inactivation under visible light, TiO2 NPs are doped with metal and non-metal elements, modified with carbonaceous nanomaterials, and coupled with other metal oxide semiconductors. Transition metals introduce a localized d-electron state just below the conduction band of TiO2 NPs, thereby narrowing the bandgap and causing a red shift of the optical absorption edge into the visible region. Silver nanoparticles of doped TiO2 NPs experience surface plasmon resonance under visible light excitation, leading to the injection of hot electrons into the conduction band of TiO2 NPs to generate reactive oxygen species (ROS) for bacterial killing. The modification of TiO2 NPs with carbon nanotubes and graphene sheets also achieve the efficient creation of ROS under visible light irradiation. Furthermore, titanium-based alloy implants in orthopedics with enhanced antibacterial activity and biocompatibility can be achieved by forming a surface layer of Ag-doped titania nanotubes. By incorporating TiO2 NPs and Cu-doped TiO2 NPs into chitosan or the textile matrix, the resulting polymer nanocomposites exhibit excellent antimicrobial properties that can have applications as fruit/food wrapping films, self-cleaning fabrics, medical scaffolds and wound dressings. Considering the possible use of visible-light active TiO2 nanomaterials for various applications, their toxicity impact on the environment and public health is also addressed.

show abstract

Distribution of Graphene Oxide and TiO2-Graphene Oxide Composite in A549 Cells

Cited by 47 publications

References 32 publications

No cytotoxicity or genotoxicity of graphene and graphene oxide in murine lung epithelial FE1 cells in vitro

No cytotoxicity or genotoxicity of graphene and graphene oxide in murine lung epithelial FE1 cells in vitro

Toxicity of graphene-family nanoparticles: a general review of the origins and mechanisms

Visible-Light Active Titanium Dioxide Nanomaterials with Bactericidal Properties

Contact Info

Product

Resources

About