Distribution of glial cells in the central nervous system of the pulmonate snail <i>Megalobulimus oblongus</i> identified by means of a glial fibrillary acidic protein marker

Santos, Paula dos; Gehlen, Günther; Faccioni-Heuser, Maria Cristina; Zancan, Denise Maria; Achaval, Matilde

doi:10.1046/j.1463-6395.2002.00126.x

Cited by 17 publications

(15 citation statements)

References 28 publications

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“…Accordingly, GFAP-like immunoreactivity has been shown in enteric glia (Jessen and Mirsky, 1980), Schwann cells of unmyelinated peripheral nerve fibers (Yen and Fields, 1981;Dahl et al, 1982), lens epithelium (Hatfield et al, 1984), and Kupffer cells in the liver (Gard et al, 1985), among others. Filaments of morphological structure similar to the intermediate glial filament have been reported in the glial cells of many mammals, birds, reptiles, fishes (Dahl et al, 1985), and in some invertebrates such as annelids and mollusks (see Radojcic and Pentreath, 1979;Cardone and Roots, 1990;Riehl and Schlue, 1998;Dos Santos et al, 2002).…”

Section: Introductionmentioning

confidence: 84%

Glial fibrillary acidic protein (GFAP)‐like immunoreactivity in the visual system of the crab Ucides cordatus (Crustacea, Decapoda)

Silva¹,

Corrêa²,

Tortelote³

et al. 2004

Biology of the Cell

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Glial fibrillary acidic protein (GFAP) is the main intermediate filament protein used as a marker for the identification of astrocytes in the central nervous system of vertebrates. Analogous filaments have been observed in the glial cells of many mollusks and annelids but not in crustaceans. The present study was carried out to identify by light microscopy immunohistochemistry, immunoelectronmicroscopy and immunoblotting, GFAP-like positive structures in the visual system of the crab Ucides cordatus as additional information to help detect and classify glial cells in crustaceans. Conventional electron microscopy, light microscopy of semithin sections and fluorescence light microscopy were also employed to characterize cells and tissues morphology. Our results indicated the presence of GFAP-like positive cell processes and cell bodies in the retina and adjoining optic lobe. The labeling pattern on the reactive profiles was continuous and very well defined, differing considerably from what has been previously reported in the central nervous system of some mollusks, where a diffuse spotted fluorescence pattern of labeling was observed. We suggest that this glial filament protein may be conserved in the evolution of the invertebrate nervous systems and that it may be used as a label for some types of glial cells in the crab.

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Section: Introductionmentioning

confidence: 84%

Glial fibrillary acidic protein (GFAP)‐like immunoreactivity in the visual system of the crab Ucides cordatus (Crustacea, Decapoda)

Silva¹,

Corrêa²,

Tortelote³

et al. 2004

Biology of the Cell

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“…The sections were again washed and then incubated in either streptavidin complex (Amersham) diluted 1 : 100 or PAP (Sigma) diluted 1 : 500 in PBS for 1.5 h at room temperature. For the localization of peroxidase activity, the sections were preincubated for 10 min in 0.06% 3,3′‐diaminobenzidine tetrahydrochloride (Sigma) dissolved in PBS, and then incubated for 10 min in the same solution containing 0.001% H 2 O 2 at room temperature (Santos et al . 2002a,b).…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, M. abbreviatus displayed vimentin and GFAP immunoreaction in the CNS, which was detected in small glial bodies and their processes. In relation to their localization through any nervous ganglion, four types of glial cells were detected in this snail, using the GFAP marker: the glial lacunar network and the perineuronal glial cells were found in the cortical region, while the perisynaptic and the fibrous glial cells were found in the neuropilar or medullar region (Santos et al . 2002a,b).…”

Section: Introductionmentioning

confidence: 99%

Detection of glial fibrillary acidic protein (GFAP) and vimentin (Vim) by immunoelectron microscopy of the glial cells in the central nervous system of the snail Megalobulimus abbreviatus

Santos¹,

Gehlen²,

Faccioni-Heuser

et al. 2005

Acta Zoologica

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dos Santos, P.C., Gehlen, G., Faccioni-Heuser, M.C. and Achaval, M. 2005. Detection of glial fibrillary acidic protein (GFAP) and vimentin (Vim) by immunoelectron microscopy of the glial cells in the central nervous system of the snail Megalobulimus abbreviatus . -Acta Zoologica (Stockholm) 86 : 135-144When examined under an electron microscope, the central nervous system of Megalobulimus abbreviatus showed two types of glial cells: firstly, protoplasmic glial cells which displayed a nucleus with peripheral heterochromatin, scanty or no intermediate filaments, a developed Golgi complex, rough and smooth endoplasmic reticula, mitochondria and polymorphic lysosomes that indicate phagocytic activity of debris from the extracellular space; and, secondly, fibrous glial cells which showed numerous glial fibrillary acidic protein (GFAP) and vimentin immunoreactive intermediate filament bundles, a discrete Golgi complex, mitochondria, endoplasmic reticulum, lipid droplets and lysosomes. The contacts between the glial cells consisted of desmosomes and puncta adherentia , while those between the glial cells and the basal lamina consisted of hemidesmosomes. Both glial cell types were located in the cortex and medullary regions, however, the protoplasmic glial cells prevailed in the cortical region, while the fibrous glial cells prevailed in the medullar region. As the nervous tissue is avascular, the passage of nutrients and waste products may be facilitated by the glial labyrinthic system which is located in the cortical region. Glial processes adjacent to large and giant neurones formed a trophospongium, which seemed to be involved in a metabolic exchange between these cells. Thus, this evidence suggests that glial cells of M. abbreviatus are involved in structural support, isolation of different ganglionic areas, the formation of a microcirculatory system and an intimate metabolic relationship with neurones.

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“…NFs have not been observed in crustaceans using conventional electron microscopy, and IFs in general have not been observed in crustaceans and other arthropods by immunocytochemistry. However, numerous studies in lower vertebrates and invertebrates have revealed the presence of NF proteins similar to mammalian NF subunits (Philips et al 1983;Viancour et al 1987;Leapman et al 1997;Karabinos et al 2001) and IF like glial fibrillary acidic protein (GFAP) (Cardone and Roots 1990;Kumar et al 1996;Riehl and Schlue 1998;dos Santos et al 2002). Particularly interesting for crustacean neurobiologists was the study conducted by Weaver and Viancour (1991), representing a very thorough search for mammalian NF proteins in crayfish axons: following labeling with the commercially available anti-NF-M monoclonal antibody (NN18-clone), it revealed a polypeptide located on microtubule side-arms.…”

Section: Introductionmentioning

confidence: 99%

Identification of a neurofilament-like protein in the protocerebral tract of the crab Ucides cordatus

et al. 2004

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Neurofilaments (NFs) have not been observed in crustaceans using conventional electron microscopy, and intermediate filaments have never been described in crustaceans and other arthropods by immunocytochemistry. Since polypeptides, labeled by the NN18-clone antibody, were revealed on microtubule side-arms of crayfish, we have tested, in this study, whether proteins similar to mammalian NFs are present in the protocerebral tract (PCT) of the crab Ucides cordatus. We used immunohistochemistry for light microscopy with monoclonal antibodies against three different NF subunits, high (NF-H), medium (NF-M), and light (NF-L). Labeling was observed with the NN18-clone, which recognizes NF-M. In order to confirm the results obtained with the immunohistochemical reactions, Western blotting, using the three primary antibodies, was performed and the presence of NF-M was confirmed. The NN18-clone monoclonal antibody recognized a protein of approximately 160 kDa, similar to the mammalian NF-M protein, but NF-L and NF-H were not recognized. Conventional transmission electron microscopy was used to observe the ultrastructural components of the axons and immunoelectron microscopy was used to show the distribution of the NF-M-like polypeptides along cytoskeletal elements of the PCT. Our results agree with previous studies on crustacean NF proteins that have reported negative immunoreactions against NF-H and NF-L subunits and positive immunoreactions against the mammalian NF-M subunit. However, the protein previously referred to as P600 and recognized by the NN18-clone, has a very high molecular weight, thus, being different from mammalian NF-M subunit and from the protein revealed now in our study.

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Distribution of glial cells in the central nervous system of the pulmonate snail Megalobulimus oblongus identified by means of a glial fibrillary acidic protein marker

Cited by 17 publications

References 28 publications

Glial fibrillary acidic protein (GFAP)‐like immunoreactivity in the visual system of the crab Ucides cordatus (Crustacea, Decapoda)

Glial fibrillary acidic protein (GFAP)‐like immunoreactivity in the visual system of the crab Ucides cordatus (Crustacea, Decapoda)

Detection of glial fibrillary acidic protein (GFAP) and vimentin (Vim) by immunoelectron microscopy of the glial cells in the central nervous system of the snail Megalobulimus abbreviatus

Identification of a neurofilament-like protein in the protocerebral tract of the crab Ucides cordatus

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