“…Change of antibiotic regime was necessary only in eight cases of this collective because of microbiologically proven resistance. This supports the idea that aminopenicillins and penicillins without additive antibiotic treatment are often insufficient in OI because of the β-lactamase activity of several bacteria (Kuriyama et al, 2001;Moraes et al, 2015).…”
Section: A C C E P T E D Accepted Manuscriptsupporting
“…Change of antibiotic regime was necessary only in eight cases of this collective because of microbiologically proven resistance. This supports the idea that aminopenicillins and penicillins without additive antibiotic treatment are often insufficient in OI because of the β-lactamase activity of several bacteria (Kuriyama et al, 2001;Moraes et al, 2015).…”
Section: A C C E P T E D Accepted Manuscriptsupporting
“…From a microbiological viewpoint, it requires a comprehensive analysis of the resistance profiles among microbial isolates from endodontic infections. Recently, Moraes et al performed a systematic review to describe the presence of resistance genes to antimicrobial agents in oral environments such as saliva, dental biofilm and endodontic infections [15]. However, there is a lack of information regarding whether the microbial isolates from endodontic infections expressing these virulence factors are conveyed as resistance to antimicrobial agents.…”
Infected root canal or acute apical abscess exudates can harbour several species, including Fusobacterium, Porphyromonas, Prevotella, Parvimonas, Streptococcus, Treponema, Olsenella and not-yet cultivable species. A systematic review and meta-analysis was performed to assess resistance rates to antimicrobial agents in clinical studies that isolated bacteria from acute endodontic infections. Electronic databases and the grey literature were searched up to May 2015. Clinical studies in humans evaluating the antimicrobial resistance of primary acute endodontic infection isolates were included. PRISMA guidelines were followed. A random-effect meta-analysis was employed. The outcome was described as the pooled resistance rates for each antimicrobial agent. Heterogeneity and sensitivity analyses were performed. Subgroup analyses were conducted based upon report or not of the use of antibiotics prior to sampling as an exclusion factor (subgroups A and B, respectively). Data from seven studies were extracted. Resistance rates for 15 different antimicrobial agents were evaluated (range, 3.5-40.0%). Lower resistance rates were observed for amoxicillin/clavulanic acid and amoxicillin; higher resistance rates were detected for tetracycline. Resistance rates varied according to previous use of an antimicrobial agent as demonstrated by the subgroup analyses. Heterogeneity was observed for the resistance profiles of penicillin G in subgroup A and for amoxicillin, clindamycin, metronidazole and tetracycline in subgroup B. Sensitivity analyses demonstrated that resistance rates changed for metronidazole, clindamycin, tetracycline and amoxicillin. These findings suggest that clinical isolates had low resistance to β-lactams. Further well-designed studies are needed to clarify whether the differences in susceptibility among the antimicrobial agents may influence clinical responses to treatment.
“…After a review of the literature, as far as we know, there are no studies in the endodontic literature in which the presence of resistance genes is correlated with the different clinical symptoms of resistant infections (secondary endodontic infection). Many studies have only focused on detecting the presence of resistance genes in different oral environments, including bacteria isolated from different types of endodontic infections [35,36]. Other studies have only been dedicated to associate the presence of certain bacterial species with the presence of clinical symptoms [37].…”
Introduction. Silver nanoparticles are used in endodontics due to their antimicrobial activity, although it is considered that bacteria are unable to develop resistance to silver nanoparticles. Silver resistance genes have been related to resistance to nanoparticles and antibiotics. The presence of these resistance genes has not been studied in endodontic bacteria. The objective of this study is to report the prevalence of silver resistance genes in endodontic bacteria. Methods. The selected teeth were isolated using a rubber dam and any restoration, post, or caries was eliminated. The operative field was disinfected, and the root-filling material was removed. The samples were obtained using three sterile paper points to absorb the fluid of the root canal. The DNA from the samples and the control organism was extracted, and the detection of the silCBA resistance genes was carried out by PCR. Results. The results of this study show a high prevalence (73.3%) of silCBA silver resistance genes. The Spearman rank correlation coefficient was utilized to identify correlations between the presence of genes and clinical variables. Conclusions. This study reports a high frequency of silver resistance genes related to nanoparticle resistant from bacteria.
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