Distribution of endocrine cells displaying immunoreactivity for one or more peptides in the pancreas of the adult Vervet monkey (Cercopithecus aethiops)

Louw, Johan; Woodroof, C. W.; Wolfe-Coote, Sonia

doi:10.1002/(sici)1097-0185(199703)247:3<405::aid-ar12>3.0.co;2-t

Cited by 10 publications

(6 citation statements)

References 24 publications

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“…Average number of PP-cells for each islet is 22.7 in the duodenal lobe, and 18.12 in the splenic lobe. Our results are similar to those from other researchers (Orci et al , 1976; Louw et al , 1997). …”

Section: Discussionsupporting

confidence: 93%

“…The coexistence of glucagon- and PP-like immunoreactivity in endocrine pancreas cells has already been demonstrated in amphibians, mammals, and fish (Kaung and Elde, 1980; Kaung, 1985a, 1985b; Wolfe-Coote et al , 1988; Herrera et al , 1991; Lozano et al , 1991; Park and Bendayan, 1992; Louw et al , 1997), by comparing the immunolabeling for either antigen in adjacent ultrathin sections.…”

Section: Discussionmentioning

confidence: 91%

“…Previous studies have revealed coexistence of glucagon- and PP-like immunoreactivity in endocrine pancreas cells of frog, rat, baboon, murine, monkey, and fish (Kaung and Elde, 1980; Kaung, 1985a, 1985b; Wolfe-Coote et al , 1988; Herrera et al , 1991; Lozano et al , 1991; Park and Bendayan, 1992; Louw et al , 1997). However, those experiments were performed by staining adjacent ultrathin sections with anti-glucagon serum and anti-PP serum respectively by peroxidase antiperoxidase (PAP) or immuno-gold labeling or avidin-biotin-peroxidase method, and the situation of two kinds of positive cells were compared.…”

mentioning

confidence: 97%

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Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells

Huang¹,

Sun²,

Jiang³

et al. 2009

Eur J Histochem

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We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets. The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PP-secreting cells) were located in the periphery of the islets. However, A-cells and PP-cells had a different regional distribution. Most of A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas. In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of the pancreas. Furthermore, we found that 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining. Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 91%

mentioning

confidence: 97%

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Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells

Huang¹,

Sun²,

Jiang³

et al. 2009

Eur J Histochem

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show abstract

“…Previous studies have revealed coexistence of glucagon-and PP-like immunoreactivity in endocrine pancreas cells of frog, rat, baboon, murine, monkey, and fish (Kaung and Elde, 1980;Kaung, 1985aKaung, , 1985bWolfe-Coote et al, 1988;Herrera et al, 1991;Lozano et al, 1991;Park and Bendayan, 1992;Louw et al, 1997). However, those experiments were performed by staining adjacent ultrathin sections with anti-glucagon serum and anti-PP serum respectively by peroxidase antiperoxidase (PAP) or immuno-gold labeling or avidin-biotin-peroxidase method, and the situation of two kinds of positive cells were compared.…”

mentioning

confidence: 99%

Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells

Yh¹,

Sun²,

Jiang³

et al. 2009

Eur J Histochem

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We used immunofluorescence double staining method to investigate the cellular localization of glucagon and pancreatic polypeptide (PP) in rat pancreatic islets. The results showed that both A-cells (glucagon-secreting cells) and PP-cells (PPsecreting cells) were located in the periphery of the islets. However, A-cells and PP-cells had a different regional distribution. Most of A-cells were located in the splenic lobe but a few of them were in the duodenal lobe of the pancreas. In contrast, the majority of PP-cells were found in the duodenal lobe and a few of them were in the splenic lobe of the pancreas. Furthermore, we found that 67.74% A-cells had PP immunoreactivity, 70.92% PP-cells contained glucagon immunoreactivity with immunofluorescence double staining. Our data support the concept of a common precursor stem cell for pancreatic hormone-producing cells

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“…To analyse the specificity of the antiserum employed against BDNF, which in particular shares amino acid sequences with NT4/5, it was essential to include antibody specificity dot blotting (Larsson 1981;Louw et al 1997).…”

Section: Dot Blotmentioning

confidence: 99%

Brain-derived neurotrophic factor in higher vertebrate pancreas: immunolocalization in glucagon cells

Lucini¹,

Maruccio²,

Girolamo³

et al. 2003

Anat Embryol

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Brain-derived neurotrophic factor (BDNF) is a growth factor that belongs to the group of neurotrophins. Its amino acid sequences are well conserved during vertebrate phylogenesis. Pancreatic tissue has recently been reported to be one of the physiological sources of BDNF in humans and mice. In this study we investigated the presence and localization of BDNF immunoreactivity (IR) in the pancreas of three species of higher vertebrates: mouse, duck and lizard. BDNF IR was present in the islets and in single cells scattered in the exocrine parenchyma of all three species examined. Using double staining, BDNF IR was seen to be colocalized with glucagon IR in all the species studied. There was a total overlap of BDNF and glucagon IR in duck and lizard pancreas, and partial overlap in mouse pancreas. Our findings suggest that, as well as the primary structure, the presence and pattern of distribution of BDNF in higher vertebrates is also well conserved. Moreover, the abundance of BDNF IR in the pancreas of the species studied leads us to the suggestion that these neurotrophins could regulate the function of pancreatic innervation and/or act on pancreatic cells in a paracrine/autocrine fashion.

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Distribution of endocrine cells displaying immunoreactivity for one or more peptides in the pancreas of the adult Vervet monkey (Cercopithecus aethiops)

Cited by 10 publications

References 24 publications

Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells

Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells

Immunohistochemical localization of glucagon and pancreatic polypeptide on rat endocrine pancreas: coexistence in rat islet cells

Brain-derived neurotrophic factor in higher vertebrate pancreas: immunolocalization in glucagon cells

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