Distribution of coenzyme B12-dependent diol dehydratase and glycerol dehydratase in selected genera of Enterobacteriaceae and Propionibacteriaceae

Toraya, Tetsuo; Kuno, Sigeru; Fukui, Saburô

doi:10.1128/jb.141.3.1439-1442.1980

Cited by 86 publications

(66 citation statements)

References 8 publications

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“…Similar results were obtained for the glycerol dehydratase of C. freundii [21]. In comparison, diol dehydratases prefer 1,2-propanediol as substrate [22].…”

Section: Resultssupporting

confidence: 85%

Properties and sequence of the coenzyme B12-dependent glycerol dehydratase of Clostridium pasteurianum

Macis

1998

FEMS Microbiology Letters

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The genes encoding coenzyme B IP -dependent glycerol dehydratase of Clostridium pasteurianum were subcloned and expressed in Escherichia coli. The native molecular mass of the enzyme is 190 000 Da. The enzyme converts glycerol, 1,2-propanediol and 1,2-ethanediol to 3-hydroxypropionaldehyde, propionaldehyde and acetaldehyde, respectively, but glycerol is the preferred substrate. The nucleotide sequences of the dhaBCE genes encoding the three subunits of glycerol dehydratase and of orfZ whose function is unknown were determined. The deduced products of the dhaBCE genes with calculated molecular masses of 60 813, 19 549 and 16 722 Da, respectively, revealed high similarity to amino acid sequences of subunits of coenzyme B IP -dependent glycerol and diol dehydratases from other organisms. z

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“…Similar results were obtained for the glycerol dehydratase of C. freundii [21]. In comparison, diol dehydratases prefer 1,2-propanediol as substrate [22].…”

Section: Resultssupporting

confidence: 85%

Properties and sequence of the coenzyme B12-dependent glycerol dehydratase of Clostridium pasteurianum

Macis

1998

FEMS Microbiology Letters

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“…This enzyme seems to be entirely di¡erent from the B IP -containing dehydratases and will not be discussed here any further. In the case of enteric bacteria, B IP -dependent glycerol or diol dehydratases were detected in some strains of the genera Salmonella, Klebsiella, Enterobacter and Citrobacter [7,15,18,22,23,25,29]. Both dehydratases may occur individually or together in these organisms.…”

Section: Distribution Of Glycerol and Diol Dehydratasesmentioning

confidence: 99%

“…Growth on 1,2-propanediol induces only the diol dehydratase, whereas both dehydratases are present in anaerobically grown glycerol cells [15]. In one Klebsiella strain (ATCC 8724), the isofunctional diol dehydratase substitutes for the defective glycerol dehydratase [2,16,22].…”

Section: Distribution Of Glycerol and Diol Dehydratasesmentioning

confidence: 99%

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Biochemistry of coenzyme B12-dependent glycerol and diol dehydratases and organization of the encoding genes

Daniel

1998

FEMS Microbiology Reviews

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Glycerol and diol dehydratases exhibit a subunit composition of alpha 2 beta 2 gamma 2 and contain coenzyme B12 in the base-on form. The dehydratase reaction proceeds via a radical mechanism. The dehydratases are subject to reaction inactivation by the substrate glycerol which is caused by a cessation of the catalytic cycle because coenzyme B12 is not regenerated, instead 5'-deoxyadenosine and a catalytically inactive cobalamin are formed. The genetic organization of the dehydratase genes is quite similar in all organisms. Downstream of the dehydratase genes an open reading frame encoding a polypeptide of approximately 600 amino acids was identified which is apparently involved in the reactivation of suicide-inactivated enzyme.

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“…Suitable microorganisms are par-ticularly found among the Enterobacteriaceae. Anaerobic growth on glycerol and formation of PD is used as a feature for the identification of the genus Citrobacter, but it is also found for strains of Klebsiella [4,5]. From the chemical point of view the microbial conversion of glycerol to PD is a disproportion, of course.…”

Section: Introductionmentioning

confidence: 99%

Microbial conversion of glycerol to 1,3-propanediol

Deckwer

1995

FEMS Microbiology Reviews

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Glycerol produced by cleavage of natural fats can microbially be converted to 1,3-propanediol (PD) by Citrobacter, Klebsiella and Clostridia strains. The fermentation by C. butyricum, product recovery and purification has been investigated in detail up to the 2 m 3 scale. Estimation of product costs for a 10,000 t/a plant indicates that the microbial process is obviously more attractive than the chemical route. Presently, 1,3-propanediol has only a low market volume; however, its use for special polycondensates, in particular polyesters, could reduce glycerol surpluses and make plastics a easily biodegradable part of natural cycles.

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Distribution of coenzyme B12-dependent diol dehydratase and glycerol dehydratase in selected genera of Enterobacteriaceae and Propionibacteriaceae

Cited by 86 publications

References 8 publications

Properties and sequence of the coenzyme B12-dependent glycerol dehydratase of Clostridium pasteurianum

Properties and sequence of the coenzyme B12-dependent glycerol dehydratase of Clostridium pasteurianum

Biochemistry of coenzyme B12-dependent glycerol and diol dehydratases and organization of the encoding genes

Microbial conversion of glycerol to 1,3-propanediol

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