Distribution of bacteria within operating laboratory water purification systems

Abstract

Experiments were conducted to measure communities of bacteria within operating ultrapure water treatment systems intended for laboratory use. Samples from various locations within Milli-Q Plus and Milli-Q UV Plus systems were analyzed for populations of planktonic bacteria at weekly intervals over 3 months of operation. Relatively high initial densities of planktonic bacteria (102 to 103 bacteria per ml) were seen within both units when they were challenged with source water of poor quality, although the product water continued to be acceptable with regard to bacterial numbers, resistivity, and endotoxin concentration. Under more normal operating conditions, significant differences were seen in planktonic populations throughout the systems with excellent product water quality. A great deal of variability was observed in biofilm populations analyzed from various system surfaces after 3 months of operation. The concentrations of planktonic bacteria and biofilm densities were much lower in the unit containing a UV lamp. These findings suggest that a range of microenvironmental conditions exist within purified water systems, leading to variable populations of bacteria. However, product water of excellent quality was obtained despite the bacterial communities. The efficient operation of water purification systems is important since the microbiological quality of ultrapure water is of fundamental concern in laboratory science, medicine, and a wide range of industries. Bacterial growth and biofouling within these systems present potentially serious problems for these applications because of biodeterioration and the presence of bacteria or their products in the purified water (19, 20). Despite this significance, surprisingly little research has focused on the microbiology of these systems, and no comprehensive studies have addressed their * Corresponding author.

Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with Listeria monocytogenes

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