Identification of culturable bacteria present in haemodialysis water and fluid

Abstract

Water used to prepare haemodialysis fluid is not sterile, and its microbiological control is important for the prevention of haemodialysis-associated illness. Bacterial populations inhabiting a distribution system for haemodialysis water were studied over an 18-month period. 203 planktonic bacteria isolated on R2A medium were identified by restriction analysis and sequencing of 16S rRNA gene. A diverse bacterial community was detected, containing predominantly Gram-negative members of the Alphaproteobacteria and Betaproteobacteria, as well as representatives of the genus Mycobacterium. Ecological and clinical consequences are discussed: bacteria from the genera Novosphingobium, Pseudomonas and Sphingomonas have been described in the build-up of biofilms, and others like Acinetobacter, Mycobacterium or Brevibacterium may represent a health risk to patients under haemodialysis treatment.

Recurrent Peritonitis Due to Caulobacter crescentus as a Rare Cause of Dialysis Technique Failure

“…Our enquiries have uncovered only 1 reported case of the disease in humans caused by this bacteria: an episode of peritonitis in a patient on PD (4) in which, like in our case, it was ruled out that its isolation was due to contamination, and the source of infection could not be determined because of its ubiquitous nature (2,3). We suspected that it might be present in the PD solution bags, although this could not be proven, since they had not previously been tested.…”

Microbial communities in a coastal cave: Cova des Pas de Vallgornera (Mallorca, Western Mediterranean)

“…For the 18S rRNA amplification of eukaryotic cells the primers used were EukA (5'-AACCTGGTTGATCCTGCCAGT-3') and EukB (5'-TGATCCTTCTGCAGGTTCACCTAC-3') (Medlin et al 1988). The same PCR conditions were used as described in Gomila et al (2005). Five microliters of amplified PCR products was analyzed by electrophoresis on 1% agarose gels and stained with ethidium bromide.…”

Isolation and characterization of Streptococcus dysgalactiae from diseased Acipenser schrenckii

“…The almost complete 16S rRNA gene and partial tuf gene (encoding elongation factor Tu and used for PCR detection of Streptococcus species) were amplified by PCR with universal primers U8f (5′-AGAGTTTGATCMTGGCTCAG-3′) and U1492r (5′-TACGGYTACCTTGTTACGACTT-3′) (Gomila et al, 2005) as well as Str1 (5′-GTACAGTTGCTTCAGGACGTATC-3′) and Str2 (5′-AC GTTCGATTTCATCACGTTG-3′) (Picard et al, 2004) respectively and sequenced (Union-Gene Group, China). The 16S rRNA and tuf gene sequences were aligned online using BLAST program with those of organisms in GenBank and percentage similarities of sequences determined.…”