Distribution of alpha-synuclein in the rat cranial sensory ganglia, and oro-cervical regions

Sato, Tadasu; Yajima, Takahiro; Saijyo, Shiori; Shimazaki, Ken-ichiro; Nishitani, Tomiko; Hoshika, Tomohiro; Nishitani, Yosuke; Ichikawa, Hiroyuki; Mizoguchi, Itaru; Fukunaga, Tomohiro

doi:10.1016/j.aanat.2021.151776

Cited by 6 publications

(6 citation statements)

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“…In addition, IgA in the saliva is elevated by their agonists and suppressed by their antagonists in the rat submandibular gland (Wada et al, 2017). Previously, we have demonstrated that Syn may affect the oral and cervical immune cells (Sato et al, 2021). Macrophages and B cells in the lingual, pharyngeal, and laryngeal mucosa are innervated by Syn-containing nerve fibers from sensory neurons of the glossopharyngeal and vagal ganglia.…”

Section: Discussionmentioning

confidence: 98%

“…Syn protein is localized to the cytoplasm and synapses and is probably released from presynaptic terminals for synaptic transmission and modulation. Previous studies have also demonstrated the presence of Syn protein in the peripheral nervous system (Giasson et al, 2001; Phillips et al, 2008; Sato et al, 2021). We previously detected Syn protein in the sensory ganglia of the vagal and glossopharyngeal nerves (Sato et al, 2021).…”

Section: Introductionmentioning

confidence: 86%

“…Previous studies have also demonstrated the presence of Syn protein in the peripheral nervous system (Giasson et al, 2001; Phillips et al, 2008; Sato et al, 2021). We previously detected Syn protein in the sensory ganglia of the vagal and glossopharyngeal nerves (Sato et al, 2021). Many sensory neurons with small cell bodies contain Syn in the jugular and petrosal ganglia and the rostral part of the nodose ganglion.…”

Section: Introductionmentioning

confidence: 86%

“…Many sensory neurons with small cell bodies contain Syn in the jugular and petrosal ganglia and the rostral part of the nodose ganglion. These neurons co‐express calcitonin gene‐related peptide, a marker for small‐ to medium‐sized nociceptors in the sensory ganglion (Sato et al, 2021). Thus, Syn‐containing neurons are considered to be expressed by nociceptors and associated with nociceptive transmission in the oral, pharyngeal, and laryngeal regions.…”

Section: Introductionmentioning

confidence: 99%

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Distribution of alpha‐synuclein in rat salivary glands

Nishitani,

Mitoh,

Yajima

et al. 2024

The Anatomical Record

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Expression of alpha‐synuclein (Syn), a presynaptic neuronal protein, was immunohistochemically examined in intact rat submandibular, sublingual, and lingual glands. The submandibular gland contained abundant periductal Syn‐immunoreactive (−ir) nerve fibers. Abundant Syn‐ir varicosities were present in acini of the sublingual and serous lingual glands. By confocal laser scanning microscopy, Syn‐ir nerve fibers around smooth muscle actin (SMA)‐ir cells alone were infrequent; however, those around aquaporin‐5 (AQP5)‐ir cells alone and both SMA‐ and AQP5‐ir cells were abundant in the sublingual and serous lingual glands. SMA‐ir cells were occasionally immunoreactive for toll‐like receptor 4, a Syn receptor. Syn‐ir nerve fibers contained tyrosine hydroxylase (TH) in the submandibular gland and choline acetyltransferase (ChAT) in all examined salivary glands. In the superior cervical (SCG), submandibular, and intralingual ganglia, sympathetic and parasympathetic neurons co‐expressed Syn with TH and ChAT, respectively. SCG neurons innervating the submandibular gland contained mostly Syn. In the thoracic spinal cord, 14.7% of ChAT‐ir preganglionic sympathetic neurons co‐expressed Syn. In the superior salivatory nucleus, preganglionic parasympathetic neurons projecting to the lingual nerve co‐expressed Syn and ChAT. The present findings indicate that released Syn acts on myoepithelial cells. Syn in pre‐ and post‐ganglionic neurons may regulate neurotransmitter release and salivary volume and composition.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Distribution of alpha‐synuclein in rat salivary glands

Nishitani,

Mitoh,

Yajima

et al. 2024

The Anatomical Record

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“…The transient receptor potential cation channel subfamily V member 1 (TRPV1) responds to heat > 43°C, protons, and capsaicin (Caterina et al 1997). Small-and medium-sized sensory neurons in the sensory ganglia of the trigeminal ganglion (TG), glossopharyngeal ganglion (PG), and vagus nerves (jugular ganglion (JG) and NG) express TRPV1 (Guo et al 1999;Ichikawa and Sugimoto 2001;Hironaka et al 2014;Sato et al 2021;Yajima et al 2022). These neurons send free nerve endings to the peripheral receptive fields (Pei et al 2007;Rodd et al 2007;Sasaki et al 2013).…”

Section: Methodsmentioning

confidence: 99%

Distribution and anti-nociceptive function of endomorphin-1 in the rat cranial sensory ganglia

SATO,

YAJIMA

et al. 2024

Biomed. Res.

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Distribution of endomorphin-1 (EM-1) was immunohistochemically investigated in the rat cranial sensory ganglia. Small to medium-sized neurons in the trigeminal (TG), petrosal (PG), and jugular ganglia (JG) expressed EM-1-immunoreactivity. However, EM-1-immunoreactive (-ir) neurons were infrequent in the nodose ganglion. In the brainstem, EM-1-ir varicose fibers were detected in the superficial layer of the medullary dorsal horn and the caudal part of the nucleus tractus solitarius. By trichrome immunofluorescence analysis, approximately 70% of EM-1-ir neurons were also immunoreactive for transient receptor potential vanilloid 1 (TRPV1) in all the examined ganglia. Additionally, 56.8% of EM1-ir TG neurons and approximately 30% of EM-1-ir PG and JG neurons showed calcitonin gene-related peptide (CGRP)-immunoreactivity. By a retrograde tracing method, several TG, PG, and JG neurons innervating the facial and external ear canal skin expressed EM-1-immunoreactivity. However, EM-1-ir neurons innervating the tooth pulp, circumvallate papilla, and pharynx were relatively rare. Thus, EM-1 expression and its coexistence with TRPV1 and CGRP in the cranial sensory neurons may depend on their various peripheral targets. EM1-ir neurons probably project to the superficial layer of the medullary dorsal horn and caudal part of the nucleus tractus solitarius. EM-1 may be involved in nociceptive transmission from the skin.

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