Distribution of allele frequencies at TTN g.231054C > T, RPL27A g.3109537C > T and AKIRIN2 c.*188G > A between Japanese Black and four other cattle breeds with differing historical selection for marbling

Abstract

BackgroundMarbling defined by the amount and distribution of intramuscular fat, so-called Shimofuri, is an economically important trait of beef cattle in Japan. Our previous study detected 3 single nucleotide polymorphisms (SNPs), g.231054C > T, g.3109537C > T and c.*188G > A, respectively, in the 5' flanking region of the titin (TTN), the 5' flanking region of the ribosomal protein L27a (RPL27A) and the 3' untranslated region of the akirin 2 genes (AKIRIN2), which have been considered as positional functional candidates for the genes responsible for marbling, and showed association of these SNPs with marbling in Japanese Black beef cattle. In the present study, we investigated the allele frequency distribution of the 3 SNPs among the 5 cattle breeds, Japanese Black, Japanese Brown, Japanese Shorthorn, Holstein and Brown Swiss breeds.FindingsWe genotyped the TTN g.231054C > T, RPL27A g.3109537C > T and AKIRIN2 c.*188G > A SNPs by polymerase chain reaction-restriction fragment length polymorphism method, using 101 sires and 1,705 paternal half sib progeny steers from 8 sires for Japanese Black, 86 sires and 27 paternal half sib progeny steers from 3 sires for Japanese Brown, 79 sires and 264 paternal half sib progeny steers from 14 sires for Japanese Shorthorn, 119 unrelated cows for Holstein, and 118 unrelated cows for Brown Swiss breeds. As compared to the frequencies of the g.231054C > T T, g.3109537C > T T and c.*188G > A A alleles, associated with high marbling, in Japanese Black breed that has been subjected to a strong selection for high marbling, those in the breeds, Japanese Shorthorn, Holstein and Brown Swiss breeds, that have not been selected for high marbling were null or lower. The Japanese Brown breed selected slightly for high marbling showed lower frequency than Japanese Black breed in the g.3109537C > T T allele, whereas no differences were detected between the 2 breeds in the frequencies of the g.231054C > T T and c.*188G > A A alleles.ConclusionsBased on this finding, we hypothesized that the pressure of the strong selection for high marbling in Japanese Black breed has increased the frequencies of the T, T and A alleles at the TTN g.231054C > T, RPL27A g.3109537C > T and AKIRIN2 c.*188G > A SNPs, respectively. This study, together with the previous association studies, suggested that the 3 SNPs may be useful for effective marker-assisted selection to increase the levels of marbling.

SNPs in AKIRIN2, TTN, EDG1 and MYBPC1 genes are associated with growth-related traits in Chinese Qinchuan cattle

“…To further analyze the distribution of the well-characterized allele of each SNP in the cattle populations based on the different genetic background, the MGC, MGG, QC, LX, WL and LL of this study as well as the JB, JBR, JSH, HOL, BS and KN [19,[21][22][23] were divided into six groups which including North China (MGC and MGG) [20], Central China (QC and LX) [20], South China (WL and LL) [20], Japanese cattle group (JB, JBR and JSH), European cattle group (HOL and BS), and Korean cattle (KN) (Figure 2; Table 4). At the c.*188G>A locus in AKIRIN2, the well-characterized A allele frequency in the JG and KN was obviously higher than the other cattle groups ( Fig.…”

“…Therefore, the objectives of this study reported herein were to: (1) perform association analyses between these SNPs in the AKIRIN2, TTN, EDG1 and MYBPC1 genes and growth-related traits in QC, (2) investigate the genetic diversity of these SNPs in three Chinese typical cattle groups (including QC, Mongolia cattle (MG), Luxi (LX), Wuling (WL) and Longlin (LL) cattle breeds), and (3) compare the frequencies of the well-characterized alleles of these SNPs in Chinese cattle breeds to those of JB, Japanese Brown (JBR), Japanese Short Horn (JSH), Holstein (HOL), Brown Swiss (BS) [21][22][23], and KN breeds [19].…”

Genetic polymorphism analysis of 5' untranslated region of thyroglobulin gene in Bali cattle (Bos javanicus) from three different regions of Indonesia

“…Wagyu cattle breeds were regarded as Bos taurus type that carrying T allele, and the consequently on its inheritances (Casas et al, 2007;Van Eenennaam et al, 2007). This occurance may caused by strong selection in Wagyu breed for marbling traits as well as on alleles of other candidate genes associated with high marbling (Watanabe et al, 2011).…”

Expression and purification of porcine Akirin2 in Escherichia coli

“…In mammals, Akirin2 is considered to be closely related to innate immune response and embryonic development (Goto et al, 2008;Macqueen and Johnston, 2009;Beutler and Moresco, 2008). There is a body of recent evidence suggesting that Akirin2 plays a crucial role in skeletal myogenesis (Macqueen and Johnston, 2009;Macqueen et al, 2010;Chen et al, 2013), and the Akirin2 gene is regarded as a positional functional candidate for the gene responsible for marbling (Sasaki et al, 2009;Watanabe et al, 2011;Kim et al, 2013).…”