ABSTRAKVariasi nukleotida g.422C>T pada gen thyroglobulin dibagian 5'untranslated region (5'UTR) (disebut TG5) telah dilaporkan mempunyai asosiasi dengan tingkat kandungan lemak intramuskuler atau marbling pada sapi potong. Penelitian ini ditujukan untuk mengkonfirmasi keragaman genetik gen TG5 pada sapi Bali dari tiga wilayah sumber bibit yang berbeda di Indonesia. Sebanyak 200 ekor sapi Bali telah dilakukan genotyping pada gen TG5 menggunakan metode polymerase chain reaction-restriction fragment lenght polymorphism (PCR-RFLP) dan analisis sekuen. Hasil penelitian menunjukkan bahwa TG5 terkonfirmasi monomorfik pada sapi Bali dimanapun wilayah asalnya. Selain itu, sebanyak sembilan kandidat SNP ditemukan di dalam gen TG bagian 5'UTR pada sapi Bali dibandingkan dengan sekuens Genbank, meskipun tidak ditemukan variasi SNP di dalam sampel sapi Bali yang diteliti. Marker genetik lain dari gen TG perlu dieksplorasi dan diverifikasi keragamannya pada sapi Bali. Sembilan kandidat SNP juga perlu diverifikasi dan divalidasi lebih lanjut dengan sampel yang lebih besar untuk dapat dikatakan sebagai SNP baru antara sapi Bali dengan referensi sekuen dari GenBank. Kata kunci : gen thyroglobulin, marbling, polimorfisme genetik, sapi Bali, 5'-untranslated region ABSTRACTThe g.422C>T nucleotide variations in the 5' untranslated region (5'UTR) of TG gene (called as TG5) has been reported to be associated with level in intramuscular fat (IMF) content or marbling in beef cattle. The objective of this study was to confirm genetic polymorphism of TG5 gene in Bali cattle populations from three different regions as the main resources of Bali cattle in Indonesia. A total of 200 head of Bali cattle have been performed genotyping on TG5 gene using polymerase chain reactionrestriction fragment lenght polymorphism (PCR-RFLP) method and sequence analysis. Results of the study confirmed that TG5 was monomorphic in Bali cattle wherever their origin regions. Moreover, nine candidate SNPs were detected within 5'UTR of TG gene in Bali cattle compared to Genbank reference sequences, although no SNP variations among Bali cattle sample studied. The new other genetic markers within an entire TG gene suggested to be explored and verified for their polymorphisms in Bali cattle. (S. Anwar et al.) 5'UTR of Thyroglobulin Gene Polymorphism in Bali Cattle 175The nine candidate SNPs were also required further verification and validation in a larger sample to be regarded as new SNPs between Bali cattle and Genbank reference sequences.
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