Distribution and phylogenetics of whiteflies and their endosymbiont relationships after the Mediterranean species invasion in Brazil

Moraes, Letícia Aparecida de; Müller, Cristiane; Bueno, Regiane Cristina Oliveira de Freitas; Santos, António M.; Bello, Vinicius Henrique; Marchi, Bruno Rossitto De; Watanabe, Luís Fernando Maranho; Marubayashi, Julio Massaharu; Santos, Beatriz Rosa Pinheiro dos; Yuki, Valdir Atsushi; Takada, Hélio Minoru; Barros, Danielle Ribeiro de; Neves, C. G.; Silva, Fábio Nascimento da; Gonçalves, Mayra Juline; Ghanim, Murad; Boykin, Laura M.; Pavan, Marcelo Agenor; Krause‐Sakate, Renate

doi:10.1038/s41598-018-32913-1

Cited by 58 publications

(78 citation statements)

References 79 publications

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“…In Brazil, the frequencies of Hamiltonella and Rickettsia remained very similar in B. tabaci MEAM1 species from 2015 to 2017. However, the same study reported the increasing of infection of Hamiltonella, Rickettsia and Wolbachia in MED species individuals from 2015 to 2017 [32]. In laboratory conditions, Rickettsia is vertically transmitted by female whiteflies at high rates ranging from 98%-99% [31,33].…”

Section: Discussionmentioning

confidence: 92%

Bacterial Endosymbiont Diversity among Bemisia tabaci (Hemiptera: Aleyrodidae) Populations in Florida

Marchi

Smith

2020

Insects

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The sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a pest of many economically important agricultural crops and a vector of plant viruses. Bemisia tabaci harbors facultative endosymbiont species that have been implicated in pest status, including tolerance to insecticides, virus transmission efficiency and tolerance to high-temperatures. The facultative endosymbionts reported in B. tabaci include Arsenophonus, Hamiltonella, Wolbachia, Cardinium, Fritschea and Rickettsia. We collected whitefly populations from weed and crop hosts in south Florida and identified the whitefly species as well as the facultative endosymbionts present in these populations by molecular analysis. In addition, a phylogenetic analysis of whiteflies and their endosymbionts was performed. The only facultative endosymbionts found among the B. tabaci populations collected in Florida were Hamiltonella and Rickettsia. The phylogenetic analysis revealed the low genetic diversity of whiteflies and their endosymbionts. Additionally, the phylogenetic tree clustered Rickettsia from Florida in the R1 genetic group. The results will aid to understand the role of the bacterial endosymbionts in the whitefly host.

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Section: Discussionmentioning

confidence: 92%

Bacterial Endosymbiont Diversity among Bemisia tabaci (Hemiptera: Aleyrodidae) Populations in Florida

Marchi

Smith

2020

Insects

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“…In Brazil, the variability in the secondary endosymbionts found in MED is quite large and may be explained by the recent and different introductions of this species into the country (Moraes et al, 2018). MED populations typically harbor Hamiltonella, Rickettsia, Wolbachia, Cardinium, and Arsenophonus (Gueguen et al, 2010;Czosnek and Ghanim, 2016).…”

Section: Discussionmentioning

confidence: 99%

Outbreaks of Bemisia tabaci Mediterranean species in vegetable crops in São Paulo and Paraná States, Brazil

Bello

Watanabe

Fusco

et al. 2020

Bull. Entomol. Res.

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AbstractThe whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is one of the most important agricultural pests and virus vectors worldwide. Bemisia tabaci is considered a complex of cryptic species with at least 44 species. Among them, the species Middle East-Asia Minor 1 (MEAM1, formerly B biotype) and Mediterranean (MED, formerly Q biotype) are the most important, and they have attained global status. In Brazil, MEAM1 was first reported in the 1990s and is currently the predominant species in the country, meanwhile, MED was recently reported in the South and Southeast regions and was found to be mainly associated with ornamental plants. Currently, an increasing problem in the management of whitefly infestations in greenhouses associated with bell pepper was observed in São Paulo State, Brazil. The whiteflies were collected and identified based on a microsatellite locus (primer pair BEM23F and BEM23R) and the mitochondrial cytochrome oxidase I gene followed by restriction fragment length polymorphism analysis and sequencing. We observed that MED was the predominant species collected on bell pepper, but it was also found on tomato, cucumber, eggplant, and weeds grown in greenhouses. In open field, we found MED on tomatoes, bell peppers, and eggplants. In addition, MED was identified in Goiás State in association with ornamental plants. The begomovirus Tomato severe rugose virus and the crinivirus Tomato chlorosis virus was detected on bell pepper and tomato, respectively. Only MED specimens were found associated with the virus-infected plants. Moreover, we also investigated the endosymbionts present in the MED whiteflies. The collected populations of B. tabaci MED harbored a diversity of secondary endosymbionts, with Hamiltonella (H) found predominantly in 89 specimens of the 129 tested. These results represent a new concern for Brazilian agriculture, especially for the management of the newly introduced whitefly MED species, which must be implemented to limit the spreading and establishment of this pest in different crops in this country.

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“…Although whitefly diversity in Brazil has been surveyed extensively in recent years (49)(50)(51)(52)(53)(54), no study has been carried out specifically to explore the composition of whitefly communities colonizing cassava. Those studies carried out in other crops demonstrated that B. tabaci MEAM1 is the predominant species across Brazil in crops such as common bean, cotton, pepper, tomato and soybean.…”

Section: Specific Associations Between Endemic Populations Of B Tabamentioning

confidence: 99%

“…Those studies carried out in other crops demonstrated that B. tabaci MEAM1 is the predominant species across Brazil in crops such as common bean, cotton, pepper, tomato and soybean. Furthermore, B. tabaci MED, which was recently introduced in Brazil, has quickly spread and currently is present in five states from the South and Southeast regions (52)(53)(54). A small number of whitefly samples from cassava were analyzed in those studies, with B. tuberculata and Tetraleurodes acaciae prevalent and detected exclusively in cassava (51,54).…”

Section: Specific Associations Between Endemic Populations Of B Tabamentioning

confidence: 99%

Assessing whitefly diversity to infer about begomovirus dynamics in cassava in Brazil

Xavier

Nogueira

Bello

et al. 2020

Preprint

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Plant virus ecology is strongly dependent on that of its vector. The necessity of a competent vector for transmission is a primary ecological factor driving the host range expansion of plant arthropod-borne viruses, with vectors playing an essential role in promoting disease emergence. Cassava begomoviruses severely constrain cassava production in Africa. Curiously, begomoviruses have never been reported in cassava in South America, the center of origin for this crop. It has been hypothesized that the absence of a competent begomoviruses vector that efficiently colonizes cassava is the reason why begomoviruses have not emerged in South America. To test this hypothesis, we performed a country-wide whitefly diversity study in cassava in Brazil. Adults and/or nymphs of whiteflies were collected from sixty-six cassava fields across twelve states representing the main agroecological zones of the country. A total of 1,385 individuals were genotyped based on partial mitochondrial cytochrome oxidase I (mtCOI) sequences. A high species richness was observed, with five previously described species and two putative new ones. The most prevalent species were Tetraleurodes acaciae and Bemisia tuberculata, representing over 75% of the analyzed individuals. Although we detected, for the first time, the presence of Bemisia tabaci Middle East-Asia Minor 1 (BtMEAM1) colonizing cassava in Brazil, it was not prevalent. The species composition varied across regions, with fields in the Northeast region showing a higher diversity. These results expand our knowledge of whitefly diversity in cassava and support the hypothesis that begomovirus epidemics have not occurred in cassava in Brazil due to the absence of competent vector populations. However, they indicate an ongoing adaptation process of BtMEAM1 to cassava, increasing the likelihood of begomovirus emergence in this crop in the near future.

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Distribution and phylogenetics of whiteflies and their endosymbiont relationships after the Mediterranean species invasion in Brazil

Cited by 58 publications

References 79 publications

Bacterial Endosymbiont Diversity among Bemisia tabaci (Hemiptera: Aleyrodidae) Populations in Florida

Bacterial Endosymbiont Diversity among Bemisia tabaci (Hemiptera: Aleyrodidae) Populations in Florida

Outbreaks of Bemisia tabaci Mediterranean species in vegetable crops in São Paulo and Paraná States, Brazil

Assessing whitefly diversity to infer about begomovirus dynamics in cassava in Brazil

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