Distribution and molecular characterization of the root endophyte Phialocephala fortinii along an environmental gradient in the boreal forest of Alberta

Addy, H. D.; Hambleton, Sarah; Currah, Randolph S.

doi:10.1017/s0953756200002896

Cited by 78 publications

(56 citation statements)

References 29 publications

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“…Phialocephala species and allied taxa often are isolated from roots and decayed wood in heathlands, forests and alpine ecosystems across north temperate and boreal regions, and as a group they display remarkable ecological plasticity (Addy et al 2000, Grünig et al 2009). The most studied belong to the PAC, which frequently are isolated from the cortex of primary roots and the periderm of lignified roots of ericaceous plants (e.g.…”

Section: Introductionmentioning

confidence: 99%

Sexual and asexual states of some endophyticPhialocephalaspecies ofPicea

2016

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Unidentified DNA sequences in isolationbased or culture-free studies of conifer endophytes are a persistent problem that requires a field approach to resolve. An investigation of foliar endophytes of Picea glauca, P. mariana, P. rubens and Pinus strobus in eastern Canada, using a combined field, morphological, cultural and DNA sequencing approach, resulted in the frequent isolation of Phialocephala spp. and the first verified discovery of their mollisia-like sexual states in the field. Phialocephala scopiformis and Ph. piceae were the most frequent species isolated as endophytes from healthy conifer needles. Corresponding Mollisia or mollisioid sexual states for Ph. scopiformis, Ph. piceae and several undescribed species in a clade containing Ph. dimorphospora were collected in the sampling area and characterized by analysis of the nuc internal transcribed spacer rDNA (ITS) and gene for the largest subunit of RNA polymerase II (RPB1) loci. Four novel species and one new combination in a clade containing Ph. dimorphospora, the type of Phialocephala, are presented, accompanied by descriptions of apothecia and previously undocumented synanamorphs. An epitype culture and corresponding reference sequences for Phialocephala dimorphospora are proposed. The resulting ITS barcodes linked with robust taxonomic species concepts are an important resource for future research on forest ecosystems and endophytes.

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Section: Introductionmentioning

confidence: 99%

Sexual and asexual states of some endophyticPhialocephalaspecies ofPicea

2016

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“…By ITS sequence based genotyping we found our isolate Rac56 to be similar to P. fortinii, (Ascomycota, pezizomycotina, helotiales) a typical DSE fungus which is a widespread fungal endophyte of plants [16,18]. Several reports have shown that P. fortinii exhibits versatile capacities of promoting elemental nitrogen (N) and phosphorus (P) absorption by host plants [25] and synthesizing glucose [26] by co-existing with host roots.…”

Section: Discussionmentioning

confidence: 81%

“…The rDNA sequence of Rac56 (KJ542292, 519 bp) and its closest related species available at GenBank were selected for construction of NJ phylogenetic tree using MEGA 4.0 program after sequence alignment with Clustal W. The endophytic fungus Rac56 was found to be closely related to Phialocephala fortinii (AY394915 and AY394921) (Figure 2c) with 99% homology. The morphological characteristic described above corresponded to anamorphic P. fortinii [16][17][18][19][20], which led to its designation as P. fortinii.…”

Section: Identification Of Rac56 As the Ideal Sal-and Tyr-producing Fmentioning

confidence: 99%

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Potential of the Endophytic Fungus Phialocephala fortinii Rac56 Found in Rhodiola Plants to Produce Salidroside and p-Tyrosol

et al. 2016

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2-(4-Hydroxyphenyl)ehyl-β-D-glucopyranoside (salidroside) and 4-(2-hydroxyethyl)phenol (p-tyrosol) are famous food and medicine additives originally derived from alpine Rhodiola plants. Salidroside or p-tyrosol production by the endophytic fungus Rac56 (Phialocephala fortinii) was confirmed by UPLC/Q-TOF-MS and 1 H-NMR. The fermentation conditions were optimized by orthogonal design using data processing system software. The broth fermentation results showed that salidroside and p-tyrosol extraction yields from Rac56 were stable and reached 1.729˘0.06 mg and 1.990˘0.05 mg per mL of culture medium, respectively. The optimal conditions for salidroside and p-tyrosol production in fermentation culture of Rac56 were determined to be 25˝C, pH values of 7 and 5, Czapek-Dox culture medium volumes of 150 mL and 50 mL in 250 mL flasks, rotation speeds of 100ˆg and 200ˆg, and fermentation durations of 7 and 15 days, respectively. Under these optimal conditions, stable yields of 2.339˘0.1093 mg and 2.002˘0.0009 mg per mL of culture medium of salidroside and p-tyrosol, respectively, were obtained, indicating that the P. fortinii Rac56 strain is a promising source of these compounds.

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“…They do not seem to be limited to associations with plant roots. Menkis et al (2004) Further evidence is provided by Addy et al (2000) who isolated dark septate fungi across a sand to wetland gradient. They found that dark septate fungi show no preference for either extreme and thrive in both very wet and very dry conditions (Addy et al 2000).…”

Section: Dark Septate Fungimentioning

confidence: 99%

Identification and community profiling of Vaccinium membranaceum root-associated fungi over an elevation gradient in BC's eastern rocky mountains.

Gorzelak¹

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Distribution and molecular characterization of the root endophyte Phialocephala fortinii along an environmental gradient in the boreal forest of Alberta

Cited by 78 publications

References 29 publications

Sexual and asexual states of some endophyticPhialocephalaspecies ofPicea

Sexual and asexual states of some endophyticPhialocephalaspecies ofPicea

Potential of the Endophytic Fungus Phialocephala fortinii Rac56 Found in Rhodiola Plants to Produce Salidroside and p-Tyrosol

Identification and community profiling of Vaccinium membranaceum root-associated fungi over an elevation gradient in BC's eastern rocky mountains.

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