Distribution and location of Daxx in cervical epithelial cells with high risk human papillomavirus positive

Abstract

AimsTo provide the basis for further exploring the effect and its mechanism of Death domain associated protein (Daxx) on the progress of cervical carcinoma induced by human papillomavirus (HPV), the distribution and location of Daxx in cervical carcinoma with high risk HPV(HR-HPV) positive was analyzed.MethodsThe samples of normal cervical epithelial cells, cervical intraepithelial neoplasia grade I (CINI), CINII CINIII and cervical cancers were collected. Immunohistochemistry assay was used to analyze the distributions and locations of Daxx in the cervical tissue. Indirect immunoinfluorescence test was utilized to observe the locations of Daxx in Caski cells with HPV16 positive.ResultsUnder the light microscopy, the brown signals of Daxx distributed in the nuclei of normal cervical epithelial cells; Daxx mainly distributed in nuclear membrane and there were a small amount of Daxx in the nuclei in CINI. Daxx intensively distributed in the cytoplasm and cell membrane in CINII, CINIII and cervical cancer. Under fluorescent microscopy, the distribution and location of Daxx in Caski cells was similarly to that in cervical cells of CINII, CINIII and cervical cancer.ConclusionIn the progress of the cervical cancer, Daxx gradually translocates from nucleus into nuclear membrane, cytoplasm and cell membrane. Daxx locates in the cytoplasm and cell membrane in CINII, CINIII and cervical cancer.Virtual slidesThe virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/4671548951113870.

Angiomyofibroblastoma of the mediastinum

“…[4] In most female patients, AMFB arises in lower genital tract and is therefore easily noticed. Four cases of pelvic AMFB have been reported in Qiu et al's study.…”

“…Four cases of pelvic AMFB have been reported in Qiu et al's study. [4] These 4 slow-growing tumors reached a massive size and caused obstructive symptoms before they were detected. In the current report, the tumor of 6.9 × 7.4 × 9.3 cm in size was located in the posterior mediastinum.…”

“…[8] On CT imaging, AMFBs show as well-defined homogeneous intermediate intensity and moderate to strong enhancement after contrast administration. [4] On magnetic resonance imaging (MRI), homogeneous hypointensity observed on T1 and T2-weighted images may be attributable to the fibrous structures. [8] In our current report, CT described a poorly circumscribed heterogeneous soft-tissue mass located in the posterior mediastinum.…”

In vitro characterization and in vivo ultrasound molecular imaging of nucleolin-targeted microbubbles

“…Cell-surface NCL plays a critical role in carcinogenesis [2], and overexpression of NCL is correlated with aggressive tumor progression and poor prognosis [3–5]. Moreover, treatment with a nucleolin antibody can produce endothelial cell apoptosis and normalization of tumor vasculature [6].…”

“…Moreover, treatment with a nucleolin antibody can produce endothelial cell apoptosis and normalization of tumor vasculature [6]. In current clinical practice, the expression level of NCL is tested through biopsy, which is invasive and cannot map the spatial distribution [3–5, 7]. Therefore, a non-invasive method to detect and map NCL expression is desired.…”