Distribution and differences of estrogen receptor beta immunoreactivity in the brain of adult male and female rats

Zhang, Ji Qiang; Wen, Chen; Zhou, De Shan; Su, Bing

doi:10.1016/s0006-8993(02)02460-5

Cited by 193 publications

(145 citation statements)

References 49 publications

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“…While T and DHT bind with high affinity to androgen receptors (ARs; Roselli et al 1987), 3α-diol and 3β-diol bind with greater affinity to estrogen receptor beta (ERβ; Roselli et al 1987), while 3α-diol may also bind to GABA/benzodiazepine receptors (GBRs; Gee 1988). Studies implicate activation of ERβ as a mediator of cell proliferation, learning and memory (Rissman et al 2002;Zhang et al 2002), as well as reducing anxiety-like behavior. In animals administered diarylpropionitrile-a selective estrogen receptor modulator specific to ERβ and not the alphaisoform of the receptor-there is a reduction in anxiety-like behavior for the open field, elevated plus maze (EPM), elevated zero maze, social interaction tasks (Walf et al 2008(Walf et al , 2009.…”

Section: Introductionmentioning

confidence: 99%

Chronic administration of androgens with actions at estrogen receptor beta have anti-anxiety and cognitive-enhancing effects in male rats

Osborne

Edinger

Frye

2009

AGE

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Androgen levels decline with aging. Some androgens may exert anti-anxiety and cognitiveenhancing effects; however, determining which androgens have anxiolytic-like and/or mnemonic effects is of interest given the different mechanisms that may underlie some of their effects. For example, the 5α-reduced metabolite of testosterone (T), dihydrotesterone, can be further converted to 5α-androstane,17β-diol-3α-diol (3α-diol) and 5α-androstane,17β-diol-3β-diol (3β-diol), both of which bind with high affinity to the beta isomer of the intracellular estrogen receptor beta (ERβ).However, androsterone, another metabolite of T, does not bind well to ERβ. To investigate the effects of T metabolites, male rats were subjected to gonadectomy then implanted with silastic capsules of 3α-diol, 3β-diol, androsterone, or oil control. After recovery, the rats were tested in elevated plus maze (EPM), light/dark transition (LD), and Morris water maze (MWM). 3α-diol both decreased anxietylike behavior in the EPM and LD, and increased cognition in MWM, while 3β-diol improved cognition in MWM, but had no effects on anxiety behavior, compared to vehicle or androsterone. These data suggest that the actions of 3α-diol and 3β-diol at ERβ may be responsible for some of testosterone's anti-anxiety and cognitive-enhancing effects.

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Section: Introductionmentioning

confidence: 99%

Chronic administration of androgens with actions at estrogen receptor beta have anti-anxiety and cognitive-enhancing effects in male rats

Osborne

Edinger

Frye

2009

AGE

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“…The classic ER, ERα, and ERβ share ~97% of their DNA and ~60% of their ligand binding domains but have distinct N-terminals regions (Tremblay et al, 1997). While many studies have indicated that ERα may be an important modulator of reproductive function (Basu & Rowan, 2005;Connor et al, 2005;Hewitt & Korach, 2003;Shimizu et al, 2005;Walf, Rhodes, & Frye, 2004), other studies have shown that ERβ is an important modulator of cell proliferation and learning and memory (Rissman, Heck, Leonard, Shupnik, & Gustafsson, 2002;Zhang, Cai, De Zhou, & Su, 2002). ERβ KO mice, which lack functional ERβ, show impaired acquisition in the Morris Water Maze task compared to their wildtype counterparts , and show increased anxiety in the open field and elevated plus maze tasks (Krezel, Dupont, Krust, Chambon, & Chapman, 2001).…”

Section: Introductionmentioning

confidence: 99%

Androgens’ effects to enhance learning may be mediated in part through actions at estrogen receptor-β in the hippocampus

Edinger

Frye

2007

Neurobiology of Learning and Memory

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Testosterone (T) may enhance cognitive performance. However, its mechanisms are not well understood. First, we hypothesized that if T's effects are mediated in part through actions of its 5α-reduced metabolites, dihydrotestosterone (DHT) and/or 3α-androstanediol (3α-diol) in the hippocampus, then T, DHT, and 3α-diol-administration directly to the hippocampus should enhance learning and memory in the inhibitory avoidance task. In order to test this hypothesis, gonadectomized (GDX) male rats were administered T, DHT, or 3α-diol via intrahippocampal inserts immediately following training in the inhibitory avoidance task. We found that T tended to increase, and DHT and 3α-diol significantly increased, performance in the inhibitory avoidance task compared to vehicle-administered GDX rats. Second, we hypothesized that, if androgens' effects are due in part to actions of 3α-diol in the hippocampus, then systemic or intrahippocampal administration of 3α-diol should significantly enhance cognitive performance of GDX male rats. Third, we hypothesized that, if androgen metabolites can have actions at estrogen receptors (ERs) in the hippocampus, then administration of ER antisense oligonucleotides (AS-ODNs) directly to the hippocampus of GDX, 3α-diol replaced, rats would decrease learning in the inhibitory avoidance task. We found that intrahippocampal administration of AS-ODNs for ERβ, but not ERα, significantly decreased learning and memory of 3α-diol replaced rats. Together, these findings suggest that T's effects to enhance learning and memory may take place, in part, through actions of its metabolite, 3α-diol, at ERβ in the dorsal hippocampus.

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“…In these brain regions the receptors were seen only in males. Only in females β-oestrogen receptors were present in the nucleus vestibularis superior (24). One also distinguished brain regions; in which immunoreactive neurons were observed in both sexes; however, an increase in reaction in females and males was different (24).…”

Section: Discussionmentioning

confidence: 95%

Immunolocalisation of α and β Oestrogen Receptors in Basolateral Amygdala of Rabbit Males

Lonc

2012

Bulletin of the Veterinary Institute in Pulawy

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Expression of α (ER-α) and β (ER-ß) oestrogen receptors in neurons of basolateral complex of the amygdala was studied in rabbit males. The examinations were carried out on brains of 12 sexually mature rabbit males weighing 3-4 kg. An immunohistochemical method (IHC) with primary Er-α antibodies, NCL-L6F11 and Er-ß: NCL-ERbeta clone EMR02 was applied. The percentage of immunoreactive neurons was determined. Morphometric and statistical analysis of immunoreactive (ER+) neurons was carried out including the localisation of IHC reaction within the examined neurons. The main place of IHC-ER-α reaction was the cytoplasm of nervous cells, whereas in the examination of IHC-ER-β, the reaction was observed mainly within cell nucleus. The nuclei of basolateral complex are a region of relatively high percentage of ER-β+ neurons and lower percentage of ER-α+ neurons in comparison with other nuclei of the amygdala. Basolateral nucleus was characterised by a higher percentage of ER-β+ neurons in which the reaction was observed in the cytoplasm.

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Distribution and differences of estrogen receptor beta immunoreactivity in the brain of adult male and female rats

Cited by 193 publications

References 49 publications

Chronic administration of androgens with actions at estrogen receptor beta have anti-anxiety and cognitive-enhancing effects in male rats

Chronic administration of androgens with actions at estrogen receptor beta have anti-anxiety and cognitive-enhancing effects in male rats

Androgens’ effects to enhance learning may be mediated in part through actions at estrogen receptor-β in the hippocampus

Immunolocalisation of α and β Oestrogen Receptors in Basolateral Amygdala of Rabbit Males

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