No abstract

Expression of α (ER-α) and β (ER-ß) oestrogen receptors in neurons of basolateral complex of the amygdala was studied in rabbit males. The examinations were carried out on brains of 12 sexually mature rabbit males weighing 3-4 kg. An immunohistochemical method (IHC) with primary Er-α antibodies, NCL-L6F11 and Er-ß: NCL-ERbeta clone EMR02 was applied. The percentage of immunoreactive neurons was determined. Morphometric and statistical analysis of immunoreactive (ER+) neurons was carried out including the localisation of IHC reaction within the examined neurons. The main place of IHC-ER-α reaction was the cytoplasm of nervous cells, whereas in the examination of IHC-ER-β, the reaction was observed mainly within cell nucleus. The nuclei of basolateral complex are a region of relatively high percentage of ER-β+ neurons and lower percentage of ER-α+ neurons in comparison with other nuclei of the amygdala. Basolateral nucleus was characterised by a higher percentage of ER-β+ neurons in which the reaction was observed in the cytoplasm.

The aim of the study was to compare the immunoreactivity and distribution of two kinds of oestrogen receptors within the nerve tissue of the posterior part of the piriform cortex, as well as their localisation within nerve cells. The studied material consisted of 12 brains of sexually mature rabbit males weighing 3-4 kg. In order to prove the presence of α and β receptors, Er-α and Er-β antibodies were used. The examinations showed the presence of Er-α and E-rβ receptors in all layers of the piriform cortex, however, mainly in the II nd and III rd layer. The reaction occurred in the nucleus and cytoplasm of neurons. The obtained results can imply the influence of oestrogen on the functions of the examined brain area in rabbits, which in human is responsible for perception and categorisation of odour.

The aim of the study was to investigate the presence of α (Erα) and β (Erβ) oestrogen receptors in rabbit male's claustrum, and thus to examine whether oestrogens may influence nerve cells' functions of this structure. The material for the study was obtained from the brains of seven rabbits. To detect the presence of Erα and Erβ, specific monoclonal antibodies were used. The expression of Erα and Erβ was demonstrated in proper claustrum, as well as in the nucleus endopiriformis. For Erβ, the reaction occurred mainly in the cell nucleus, whereas Erα was localised predominantly in the neuron cytoplasm. For both Erα and Erβ, the nuclear and cytoplasmic localisation was observed in a small percentage of the examined cells. The obtained results may indicate oestrogen influence on the brain area and also suggest a further research on oestrogen-dependent pathways in males, which still remain greatly unexplored.

No abstract