Distribution and degradation of DNA from non-genetically and genetically modified soybean (Roundup Ready): Impact of soybean protein concentrate and soybean protein isolate preparation

Effect of Soybean Protein Concentrate Preparation on Copy Numbers and Structural Characteristics of DNA from Genetically Modified Soybean

“…Table 2 shows that the copy numbers of both targets from soybean kernel, defatted soybean kernel flour, and SPC precipitate 1-4 were 1.04 × 10 7 -1.18 × 10 9 , while those from soybean hull, soybean oil, and SPC supernatant 1-4 were much lower (1.27 × 10 3 -2.39 × 10 7 ). Therefore, the majority of the lectin and cp4 epsps targets were distributed in the main products after dehulling, defatting, and the first to fourth ethanol extraction, which was expected from the distribution of DNA discussed previously [14]. As proteins were also distributed more in the main products than in the by-products along the SPC preparation chain, it was deduced that the distribution of DNA was related to the distribution of protein.…”

“…SPC was prepared according to the procedures described previously [14]. A total of 100 g of raw soybean was used, and samples from each preparation procedure, i.e., dehulling, defatting, the first to fourth ethanol extraction (Figure 1), were collected, lyophilized, weighed, and stored at 4 • C for further research.…”

“…According to Du et al [14], DNAs from each sample were isolated, dispersed in DNase/RNase-Free Water (R1600, Solarbio Life Sciences, Beijing, China), and stored at −20 • C for further analysis. The UV spectrophotometry method was applied to assess the purity and quality of DNA extracts.…”

“…As the main form of soybean protein, soybean protein concentrate (SPC) is vital to the feed and food industry. Considering the possibility of GM soybean being used as SPC, entering the human food chain, and thereby arousing consumer concern, we have recently demonstrated the distribution and degradation of DNA from GM soybean event GTS 40-3-2 during SPC preparation [14]. In order to further obtain the degradation ratio of the DNA and clarify the effects of SPC preparation on the structural characteristics of transgenic DNA, the copy numbers were absolutely quantified and the nanotopography and conformation were monitored in this research.…”

An Overview of Near Infrared Spectroscopy and Its Applications in the Detection of Genetically Modified Organisms

“…Roussel et al [ 42 ] were the first to use NIRS to distinguish Roundup Ready ® from conventional soybeans. Roundup Ready ® soybean is a glyphosate resistant GM soybean (5-enol-pyruvylshikimate-3-phosphate synthase ( EPSPS gene) developed by Monsanto that accounts for more than 83% of global annual soybean production [ 85 ]. In the study, a database of around 8000 samples yielded an accuracy rate of 93% using PLS, LWR and ANN chemometric models.…”