A comparison of DNA extraction methods and PCR-based detection of GMO in textured soy protein

Ashrafi‐Dehkordi, Elham; Mazloomi, Seyed Mohammad; Hemmati, Fatemeh

doi:10.1007/s00003-020-01300-2

Cited by 3 publications

(3 citation statements)

References 35 publications

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“…Matthes et al [14] focused on the efficiency of DNA extraction methods from corn gluten of protein-rich corn-containing feed samples. Ashrafi Dehkordi et al [15] in their study for DNA extraction from soybean samples, compared phenol/chloroform methods, CTAB and modified CTAB method. Their results showed that the modified CTAB method is more promising than the other two DNA extraction methods [15].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Modified DNA Isolation Methods for the Detection of GMO in Processed Foods

AKSOY

Sönmezoğlu

2022

International Journal of Life Sciences and Biotechnology

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İşlenmiş gıda numunelerindeki yüksek oranda bozulmuş DNA içeriği, GDO'ların tespitinde verimi sınırlamaktadır. Genel olarak, literatürde transgenik bitki tohumlarından veya ham maddelerden geleneksel DNA izolasyon teknikleri mevcuttur, ancak işlenmiş gıda örneklerinden DNA izolasyon teknikleri ile ilgili çalışmalar sınırlıdır. Ayrıca, birçok işlenmiş gıda ürünü, çok sayıda karmaşık bitki veya hayvan kaynaklarından elde edilen genomik DNA içerir. Bu çalışma bisküvi, kek, kraker, mısır cipsi ve un gibi işlenmiş gıdaların yüksek kaliteli DNA izolasyonu için bazı faydalı modifikasyonlar önermektedir. Bu amaçla, işlenmiş gıdaları incelemek için GDO analizinin ilk adımı olan gıda örneklerinden yüksek moleküler ağırlıklı ve kaliteli DNA elde etmek için izolasyon protokolleri araştırılmıştır. Elde edilen DNA örneklerinden beklenen uzunluktaki hedef organizmanın gen bölgesini kontrol etmek ve bunları DNA izolasyon verimleriyle karşılaştırmak için soya fasulyesi ve mısıra özgü primerler ile PCR tespiti yapılmıştır. Test edilen dört izolasyon yöntemi arasında, değiştirilmiş Wizard-CTAB yöntemi, test edilen gıda ürünlerinin çoğunda daha iyi sonuçlar vermiştir. Sonuçlar, modifiye Wizard-CTAB'nin mısır ve soya fasulyesine özgü genler ve GDO tespiti ile ilgili çalışmalar için farklı gıda ürünlerinde seçim için kullanılabileceğini göstermiştir.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Ashrafi Dehkordi et al [15] in their study for DNA extraction from soybean samples, compared phenol/chloroform methods, CTAB and modified CTAB method. Their results showed that the modified CTAB method is more promising than the other two DNA extraction methods [15].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Modified DNA Isolation Methods for the Detection of GMO in Processed Foods

AKSOY

Sönmezoğlu

2022

International Journal of Life Sciences and Biotechnology

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“…However, the traditional methods of extracting DNA from plant tissues are complex and time-consuming. An example of such methods is the cetrimonium bromide (CTAB) precipitation method, which is widely used in the extraction of nucleic acids from plant samples [ 15 – 17 ]. The CTAB extraction process mainly includes mechanical grinding, chemical reagent cell lysis, temperature-assisted incubation, and organic-phase nucleic acid extraction.…”

Section: Introductionmentioning

confidence: 99%

Rapid identification and quantitation of single plant seed allergen using paper-based microfluidics

Sun

Liu²,

Niu³

et al. 2022

PLoS ONE

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Nucleic acid amplification is a sensitive and powerful tool for allergen detection. However, it is limited due to the relatively cumbersome methods required to extract nucleic acids from single plant seed allergen (e.g. peanut and soybean). In view of this, an approach of extracting nucleic acid with untreated glass-fiber paper (paper-based microfluidics) was applied for nucleic acid capture and purification from plant seed allergen and commercial products. After cut by hollow cylindrical cutter, a certain size the paper chip it used to absorb DNA. And this paper-based microfluidics with DNA was directly applied for amplification by loop-mediated isothermal amplification (LAMP). To evaluate the adsorption performance of paper chip to DNA, CTAB and SDS method were used as comparisons. From amplification results, the established technique has good specificity, high repeatability (C.V. values are 4.41% and 6.17% for peanut and soybean) and favorable sensitivity (7.39 ng/μL or peanut and 6.6 ng/μL for soybean), and successfully used for commercial products (2 kinds of candy and 2 kinds of cakes containing peanut, and 2 kinds of drinks, candy and 2 kinds of biscuits containing soybean). This speed and flexible detection method makes it suit for applications in point-of-care (POC) detection at different scenario, such as custom house and import port.

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Detection of genetically modified organisms in food products commercialized in Mozambique

Joelma¹,

Pedro²,

Amélia³

et al. 2022

Afr. J. Food Sci.

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A comparison of DNA extraction methods and PCR-based detection of GMO in textured soy protein

Cited by 3 publications

References 35 publications

Comparison of Modified DNA Isolation Methods for the Detection of GMO in Processed Foods

Comparison of Modified DNA Isolation Methods for the Detection of GMO in Processed Foods

Rapid identification and quantitation of single plant seed allergen using paper-based microfluidics

Detection of genetically modified organisms in food products commercialized in Mozambique

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