Distribution Analysis of IS231-like Sequences Among Bacillus thuringiensis Serovars Inferred from Restriction Fragment Length Polymorphisms

Joung, K.‐B.; Côté, Jean-Charles

doi:10.1007/s00284-003-4036-z

Cited by 6 publications

(6 citation statements)

References 17 publications

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“…It was also proved that the distribution of IS231 was wide and no strain, serotype or cry gene-specific in the B. thuringiensis strains. This data agreed with the result reported by other authors [5,25].…”

Section: Occurrence Of Is231 Elementssupporting

confidence: 94%

“…The distribution of many insertion sequences within and between various bacterial species had often been investigated as part of the initial characterization of a new element, usually by simple Southern hybridization [8,25]. However, since the IS 231 elements were delimited by conserved and specific inverted repeats, the use of primers corresponding to these ends allowed their rapid isolation by PCR amplification.…”

Section: Resultsmentioning

confidence: 99%

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Identification, distribution pattern of IS231elements inBacillus thuringiensisand their phylogenetic analysis

Huang

Liu

Song

et al. 2004

FEMS Microbiology Letters

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In order to better understand the fundamental biology of Bacillus thuringiensis, a single oligonucleotide primer (5'-CATSSCCATCAASYTAAVR-3') was used to investigate the distribution pattern of IS231 elements in B. thuringiensis by PCR. The results indicated that IS231 elements appeared in 20 standard strains and 107 of 111 China isolates. Three novel IS231, IS231J, IS231O and IS231Q, five variants and a mobile insertion cassette MICBth4 were cloned from eight standard strains of B. thuringiensis, respectively. Interestingly, BLAST analysis revealed that the 5' end of novel IS231J shared 99% identity in 495-bp with a DNA segment adjacent to the 3' end of B. thuringiensis vip1Ac gene (GenBank Accession No. ). Two phylogenetic trees of IS231 elements were constructed and analyzed by neighbor-joining and UPGMA methods from PHYLIP 3.6b program, respectively.

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Section: Occurrence Of Is231 Elementssupporting

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Identification, distribution pattern of IS231elements inBacillus thuringiensisand their phylogenetic analysis

Huang

Liu

Song

et al. 2004

FEMS Microbiology Letters

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“…The d-endotoxins are encoded by cry genes. IS are widely distributed in B. thuringiensis (Léonard et al 1997;Joung and Cô té 2003). Several cry genes have been shown to be adjacent to specific IS (Mahillon et al 1994;Mahillon and Chandler 1998).…”

Section: Introductionmentioning

confidence: 96%

Unusual organization associated to a tandem of IS231may yield two peculiar cloverleaf secondary structures

Xu¹,

Côté²

2007

DNA Sequence

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A 5.7-kb EcoRI fragment was cloned from plasmid DNA of Bacillus thuringiensis strain M15. It contains two insertion sequences (IS), IS231M2 and -M1 in the 5'-3' order, arranged in tandem, in same orientation, separated by a 540-bp region. The primary structure is typical of a composite transposon, here of 3847 bp in length, for which the name Tn231M is proposed. Each IS is delimited by 18-bp inverted repeats (IR), and flanked by 11-bp direct repeats (DR). Both IS share 99.3% nucleotide identities. IS231M1 has a single open reading frame (ORF) which encodes a putative 477-amino-acid transposase. IS231M2 has two smaller ORFs: ORF1 and ORF2, which could code for polypeptides of 329 and 118 amino acids in length, respectively. Further analysis reveals that the regions upstream of IS231M2, and downstream of -M1, and the 540-bp region, contain additional pairs of IR and DR. Interestingly, potential annealing between all pairs of IR and DR could generate two unusual cloverleaf secondary structures.

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“…For example, SigD, the motility-specific sigma factor, and the DegU/DegS two-component system, are absent from the species of the B. cereus sensu lato group; while the virulence regulator PlcR, which plays an important role in B. cereus and B. thuringiensis physiology 14,15 , is absent in B. subtilis. Besides, diverse Insertion Sequences (ISs) have been described in different B. thuringiensis isolates [16][17][18][19] , while only limited number of ISs have been reported in B. subtilis 20 . ISs are the simplest transposable elements and play important role in shaping their host genomes 21 .…”

Section: Introductionmentioning

confidence: 99%

Species and condition shape the mutational spectrum in experimentally evolved biofilms

Wang

Liu

et al. 2022

Preprint

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Laboratory experimental evolution provides a powerful tool for studying microbial adaptation to different environments. To understand the differences and similarities of the dynamic evolutionary landscapes of two model species from the Bacillus genus as they adapt to abiotic and biotic surfaces, we revived the archived population samples from our four previous experimental evolution studies and performed longitudinal whole-population genome sequencing. Surprisingly, higher number of mutations, higher genotypic diversity, and higher evolvability were detected in the biotic conditions with smaller population size. Different adaptation strategies were observed in different environments within each species, with more diversified mutational spectrum detected in biotic conditions. The insertion sequences of Bacillus thuringiensis are critical for its adaptation to the plastic bead-attached biofilm environment, but insertion sequence mobility was a general phenomenon in this species independent of the selection condition. Additionally, certain parallel evolution has been observed across species and environments, particularly when two species adapt to the same environment at the same time. Further, the higher degree of genetic diversification observed in biotic selective environments indicates an increased spatial niche heterogeneity and a nutritional source difference created by the plant host that provided a strong strength of selection during the adaptation process. Together, these results provide the first comprehensive mutational landscape of two bacterial species biofilms that is adapted to an abiotic and biotic surface.

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Distribution Analysis of IS231-like Sequences Among Bacillus thuringiensis Serovars Inferred from Restriction Fragment Length Polymorphisms

Cited by 6 publications

References 17 publications

Identification, distribution pattern of IS231elements inBacillus thuringiensisand their phylogenetic analysis

Identification, distribution pattern of IS231elements inBacillus thuringiensisand their phylogenetic analysis

Unusual organization associated to a tandem of IS231may yield two peculiar cloverleaf secondary structures

Species and condition shape the mutational spectrum in experimentally evolved biofilms

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