Distribución relativa de genotipos de virus papiloma humano en muestras de carcinoma escamoso de laringe

A, Mariela Torrente; A, Loreto Yeomans; P, Maritza Abud; F, José Manuel Ojeda

doi:10.4067/s0718-48162009000200002

Cited by 2 publications

(2 citation statements)

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“…The reports of frequency of HPV DNA in HNSCC have varied between 14% and 60%, depending on tumour site, specimens, number of cases included, and methods applied for molecular analysis [ 15 ]. The techniques commonly employed for HPV detection is PCR with generic primers MY09/MY11 and GP5+/GP6+, considered as one of the most sensitive; however the length of the amplified region (450pb) is frequently a limitation [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Human papillomavirus detection in head and neck squamous cell carcinoma

Vietia¹

2014

ecancer

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IntroductionHuman Papillomavirus (HPV) has been associated with benign and malignant lesions in different epitheliums. The relationship between specific genotypes of high-risk HPV and some human cancers is well established. The aim of this work was to detect the HPV genotypes present in head and neck squamous cell carcinoma (HNSCC).MethodsWe evaluated 71 samples of patients with histopathological diagnosis of HNSCC. The DNA extraction was conducted with the QIAGEN commercial kit. HPV detection and genotyping were performed by reverse hybridisation (INNO-LiPA) following the commercial specifications.ResultsThe mean age of the patients evaluated was 60.7 ± 13.11 years. The distribution of the lesions included 25 (35.20%) cases of squamous cell carcinoma (SCC) of the oral cavity, 23 (32.39%) of larynx, 16 (22.50%) of the oropharynx, 4 (5.63%) of paranasal sinus, and 2 (2. 80%) cases of SCC of the nostril. Of the patients, 78.9% were males, and of these 76% were tobacco users and 67.6% were alcohol consumers. The viral DNA was detected in 67.6% of the samples. The oral cavity and the larynx were the highest HPV-positivity sites with 35.40% and 29.10% respectively. The most frequent genotype was 16 as single infection (18.70%), or in combination with another HPV types. In the oral cavity and larynx the genotypes 16 or the combination 6 and 51 were present in 11.76% and 14.28%, respectively; and in the oropharynx the most frequent genotype was 16 in 22.50% of the cases, and in the paranasal sinus 50% presented infection with HPV-6. We observed that tumours with most advanced size and stage presented greater HPV positivity.ConclusionsThis study shows a high percentage of HPV positivity in SCC is mainly associated with high-risk HPV. It is important to highlight that viral infection, especially HPV-16, could be a risk factor in HNSCC progression.

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Section: Discussionmentioning

confidence: 99%

Human papillomavirus detection in head and neck squamous cell carcinoma

Vietia¹

2014

ecancer

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“…39 clasificaron a los pacientes en categorías de riesgo de muerte, siendo el estatus VPH positivo de bajo riesgo en caso de índice paquete año (IPA) < 10, o bien, IPA > 10 pero N no mayor a N2a; y pacientes de riesgo intermedio en aquellos con estatus VPH positivo, IPA > 10 y N2b-N3. El estudio multicéntrico ICON-S que incluyó a 1907 pacientes con carcinoma orofaríngeo VPH positivo propuso una etapificación de acuerdo con el pronóstico 40 que, posteriormente fue acogida por la 8 va edición del sistema de etapificación de la American Joint Committe on Cancer, separa en dos entidades distintas el carcinoma orofaríngeo p16 positivo y negativo (Tabla 1) 41 . Este cambio ha resultado en que la mayoría de los pacientes clasificados como etapa III-IV ahora correspondan a etapa I-II, reflejando el alto grado de supervivencia global y libre de enfermedad a los dos años postratamiento 42 .…”

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