Human papillomavirus detection in head and neck squamous cell carcinoma

Vietia, Dayahindara

doi:10.3332/ecancer.2014.475

Cited by 12 publications

(13 citation statements)

References 25 publications

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“…A high percentage of the samples were positive for HPV infection, which coincides with studies carried out by Veitía et al, 17 who reported that 66.7% of Venezuelan patients with head and neck tumors were positive for this virus, with the highest infection rate found in the oral cavity. Studies worldwide show similar results, for example Kreimer et al 18 suggest the oral cavity is the anatomical location with the greatest tendency to develop these kinds of tumors, also being a location with the highest rate of HPV infection.…”

supporting

confidence: 73%

Prevalence of HPV and EBV infection and their relationship with the p53 and PCNA expression in oral carcinoma patients.

Veitía¹,

Liuzzi

Ávila

et al. 2017

J Oral Res

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Introduction: Infection caused by potentially oncogenic viruses, such as HPV and EBV, favors the role of certain oncoproteins that can induce dysplasias and malignant lesions. Objective: To evaluate the prevalence of HPV and EBV and their relation with the expression of p53 and PCNA in patients with oral carcinoma. Methodology: Twenty-seven oral squamous cell carcinomas (OSCC) were evaluated; DNA extraction was conducted using the QIAamp DNA mini kit; viral detection was obtained using the IN-NO-LiPA kit for HPV, and nested PCR was used for EBV. The evaluation of molecular markers was performed through immunohistochemical staining. Results: The mean age of the patients was 60.55±13.94 years, and 52% of these were female. Of the patients, 59% were tobacco users and 63% were alcohol consumers. HPV was detected in 70% of the patients with the predominance of genotype 16 (60%). As for EBV infection, it was observed in 59% of cases. p53 and PCNA immunopositivity corresponded to 44% and 59%, respectively. The tongue was the anatomical location with highest positivity for both viruses as well as for the expression of molecular markers. The 48% of the cases presented infection by both viruses. Conclusion: HPV and EBV infection together with the expression of p53 and PCNA were more frequently observed in advanced stages of the disease, suggesting a more relevant role in the progression than in tumor genesis.

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supporting

confidence: 73%

Prevalence of HPV and EBV infection and their relationship with the p53 and PCNA expression in oral carcinoma patients.

Veitía¹,

Liuzzi

Ávila

et al. 2017

J Oral Res

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“…In relation to the anatomical location, the oral cavity appeared to have a greater HPV positivity (66.7%) compared to the oropharynx (33.3%). Our ndings are similar to those reported by Vietía et al [22].…”

Section: Discussionsupporting

confidence: 93%

“…The high incidence of high-risk HPV genotypes, especially genotypes16 and 18, have been well established and reported in several studies at all the anatomical location. (22,23). However, the role of HPVs as etiological factor for HNSCCs is still under debate.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of HPV Infection in Head and Neck Cancer Patients in Egypt: National Cancer Institute Experience.

Salem¹,

Bahaa

Rouby

et al. 2020

Preprint

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Background: The new classification of Head and Neck Squamous Cell Carcinoma (HNSCC) according to their association with human papillomavirus (HPV) infection into HPV positive tumors with good prognosis and HPV negative tumors with poor prognosis has changed the treatment strategy of HNSCCs dramatically worldwide. However, data concerning HPV infection in HNSCCs patients is still limited and there are no studies regarding HPV genotypes in HNSCC among Egyptians. Therefore, the aim of the current study is to investigate the frequency of HPV and to detect the most prevalent HPV genotypes in the patients with oral cavity and oropharyngeal carcinoma from EgyptMethods: This cross sectional study was conducted from January 2016 to November 2017. Ninety-two oral brushing samples (76 oral cavity and 16 oropharynx) were collected by cytobrushing in CYTO-fast solution. HPV-DNA was subjected to two methods which are the: (1) HPV Direct Flow CHIP system for automatic detection and genotyping (2) conventional PCR for detection of HPV using consensus primers (MYO9/MYO11). SPSS software was used for statistical analysis.Results: Out of the 92 cases assessed, HPV DNA was present in three samples only (3/92; 3.3%) and the HPV66 genotype was the only genotype detected by HPV Direct Flow CHIP System.Conclusion: The Results of this study may (1) indicate that the HPV-associated HNSCC among Egyptian patients is very low and (2) highlight the presence of HPV66 genotype in these malignancies. Other studies from other countries in general and from different governorates in Egypt are still needed to determine the exact frequency of HPV infections in head and neck cancer.

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“…HNSCC presents primarily in males, and the age of appearance is being an important factor [ 10 , 11 ]. In this investigation, the average age was maintained within the range described of 60.34 ± 14.48 years (range 19–89), and in agreement with the previous reports, this type of malignity presents most frequently between the fifth and seventh decades of life, with a mean average of 62 ± 12 years [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Association of viral load and physical status of HPV-16 with survival of patients with head and neck cancer

Veitía¹,

Liuzzi²,

Ávila³

et al. 2020

ecancer

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Introduction: Head and neck cancers (NHCs) are of multifaceted origins, and tobacco and alcohol are the primary risk factors. Currently, other factors associated with the genesis of these tumours are being considered, among these viral infections, especially human papillomavirus (HPV) infection. Objective: The objective was to evaluate HPV infection, HPV-16 E6 load and its physical status in patients with squamous cell carcinoma in the head and neck and evaluate its effects in the survival of these patients. Methodology: A total of 80 fresh biopsies of HNC were evaluated. The genetic material was extracted using the commercial kit QIAGEN. The detection and classification of HPV were carried out using INNO-LiPA, whereas the quantification and analysis of integration of the viral genome into the host cell were carried out using real-time PCR. Results: The average age of the patients included was 60.34 ± 14.48 years, with a predominance of the male gender. The most frequent HPV infection was genotype 16 (52.8%), with an average of 10 copies of the HPV-16 E6/β-globin gene. Furthermore, an integration of the viral genome in the host cell was observed in 86% of cases with a statistically significant relationship between the location of the tumour and the viral load (p < 0.05). Conclusions: HPV-16 is the most common infection, and its physical status in the host cell is the determining factor in establishing response to treatment. However, more studies are needed to demonstrate the role of HPV infection in carcinogenesis.

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Human papillomavirus detection in head and neck squamous cell carcinoma

Cited by 12 publications

References 25 publications

Prevalence of HPV and EBV infection and their relationship with the p53 and PCNA expression in oral carcinoma patients.

Prevalence of HPV and EBV infection and their relationship with the p53 and PCNA expression in oral carcinoma patients.

Prevalence of HPV Infection in Head and Neck Cancer Patients in Egypt: National Cancer Institute Experience.

Association of viral load and physical status of HPV-16 with survival of patients with head and neck cancer

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